Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F60 I1 R1 17 54.2 1827820 99.0% 1809541 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,348,285 Δ1 bp coding (628/1935 nt) rnb ← RNase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,348,2850C.100.0% 128.7 / NA 30coding (628/1935 nt)rnbRNase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/16);  total (14/16)

GATAGTTGGTGAATGCGCGAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGTCATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAGCATTTCGGTAGCGACGCCGTCTGGTGCTTCTTTTTCCAGATTATGGCGTGCA  >  NC_000913/1348153‑1348418
                                                                                                                                    |                                                                                                                                     
gATAGTTGGTGAATGCGCGAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCatat                                                                                                                               >  1:659412/1‑140 (MQ=255)
       ggTGAATGCGCGAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCtgt                                                                                                                        >  2:588112/1‑140 (MQ=255)
       ggTGAATGCGCGAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCtgt                                                                                                                        >  2:435049/1‑140 (MQ=255)
           aaTGCGCGAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTg                                                                                                                    >  2:387254/1‑140 (MQ=255)
             tGCGCGAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGc                                                                                                                  >  1:356808/1‑140 (MQ=255)
               cgcgAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCa                                                                                                                 >  2:202435/1‑139 (MQ=255)
               cgcgAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCa                                                                                                                 <  1:202435/139‑1 (MQ=255)
                        tCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATgg                                                                                                       <  2:590988/140‑1 (MQ=255)
                                 tGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAAt                                                                                              >  2:131512/1‑140 (MQ=255)
                                 tGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAAt                                                                                              <  1:563885/140‑1 (MQ=255)
                                 tGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAAt                                                                                              <  1:672207/140‑1 (MQ=255)
                                     cAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAg                                                                                          <  2:312282/140‑1 (MQ=255)
                                      cgCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAgc                                                                                         <  1:564265/139‑1 (MQ=255)
                                      aGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAgc                                                                                         <  1:649662/140‑1 (MQ=255)
                                        cTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAgcgc                                                                                       >  1:191908/1‑140 (MQ=255)
                                             tgcCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCa                                                                                  >  2:102536/1‑140 (MQ=255)
                                                cTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGAt                                                                               <  1:102536/140‑1 (MQ=255)
                                                     gCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCa                                                                          <  1:131512/140‑1 (MQ=255)
                                                     gCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCa                                                                          <  1:139446/140‑1 (MQ=255)
                                                          ccACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAAc                                                                     <  2:442630/140‑1 (MQ=255)
                                                             cgcgGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAg                                                                  >  2:443033/1‑140 (MQ=255)
                                                                       tCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCg                                                        <  2:759627/140‑1 (MQ=255)
                                                                         ggCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAg                                                      <  2:463368/140‑1 (MQ=255)
                                                                         gacAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAg                                                      <  2:602223/138‑1 (MQ=255)
                                                                            aaTCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAGCAt                                                   >  1:833982/1‑140 (MQ=255)
                                                                            aaTCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAGCAt                                                   <  1:387254/140‑1 (MQ=255)
                                                                                gCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAGCATTTCg                                               >  1:392228/1‑140 (MQ=255)
                                                                                                        tCCGGCAACGCCTTAGCGAAAAGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAGCATTTCGGTAGCGACGCCGTCTGGTGcttct                       >  2:44782/1‑140 (MQ=255)
                                                                                                                            aaGGGCGT‑ATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAGCATTTCGGTAGCGACGCCGTCTGGTGCTTCTTTTTCCAGATTATGGCGTGc   <  2:587732/140‑1 (MQ=255)
                                                                                                                             aGGGCGT‑ATCCATATCTTCTGTGCTGACACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAGCATTTCGGTAGCGACGCCGTCTGGTGCTTCTTTTTCCAGATTATGGCGTGCa  >  1:732334/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GATAGTTGGTGAATGCGCGAATTTTCGCGGCTTTGTCCAGCTTGCTGCCTTCAGCAATCCACGCGGTTGGATCGGCAATCGCCACAATCAGCTGAAGTTTGTCATCCGGCAACGCCTTAGCGAAAAGGGCGTCATCCATATCTTCTGTGCTGGCACTGTCGATGGTGACAAAATCCAGCGCGGTCAGATCTTCACGAACCAGACCTTCATCGAGCATTTCGGTAGCGACGCCGTCTGGTGCTTCTTTTTCCAGATTATGGCGTGCA  >  NC_000913/1348153‑1348418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A