Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A45 F60 I1 R1	18	98.3	3347606	98.6%	3300739	138.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,572,121	C→T	intergenic (‑76/+286)	gadB ← / ← pqqL	glutamate decarboxylase B/putative zinc peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,572,121	0	C	T	100.0%	205.2 / NA	58	intergenic (‑76/+286)	gadB/pqqL	glutamate decarboxylase B/putative zinc peptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/32); total (26/32)

ACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTCGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTC  >  NC_000913/1571989‑1572257

aCCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTa                                                                                                                                   >  2:181186/1‑140 (MQ=255)

       cGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACa                                                                                                                            <  2:692050/140‑1 (MQ=255)

       cGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACa                                                                                                                            <  1:202807/140‑1 (MQ=255)

             tCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATcc                                                                                                                      <  2:1476644/140‑1 (MQ=255)

               gAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTa                                                                                                                    <  2:947449/140‑1 (MQ=255)

                agtagtTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTaa                                                                                                                   <  2:680644/140‑1 (MQ=255)

                   agtTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGt                                                                                                                <  1:1329203/140‑1 (MQ=255)

                   agtTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGt                                                                                                                <  2:466650/140‑1 (MQ=255)

                     ttCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGtta                                                                                                              >  2:690674/1‑140 (MQ=255)

                         gACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGttattta                                                                                                          <  1:337408/140‑1 (MQ=255)

                          aCCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAAt                                                                                                                        >  2:16300/1‑125 (MQ=255)

                          aCCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAAt                                                                                                                        <  1:16300/125‑1 (MQ=255)

                          aCCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGttatttat                                                                                                         >  2:1478132/1‑140 (MQ=255)

                          aCCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGttatttat                                                                                                         >  1:593613/1‑140 (MQ=255)

                          aCCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGttatttat                                                                                                         >  2:844223/1‑140 (MQ=255)

                          aCCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGttattta                                                                                                          <  1:927808/139‑1 (MQ=255)

                          aCCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGttattta                                                                                                          >  2:927808/1‑139 (MQ=255)

                             ttAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGt                                                                                                      <  1:690674/140‑1 (MQ=255)

                                      ttACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTa                                                                                             >  1:1154342/1‑140 (MQ=255)

                                        aCTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTAcg                                                                                           <  1:263408/140‑1 (MQ=255)

                                             cttcttATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGaat                                                                                      <  1:1478132/140‑1 (MQ=255)

                                               tcttATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGaataa                                                                                    >  1:854947/1‑140 (MQ=255)

                                                       aTTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATAtttttt                                                                            >  2:968747/1‑140 (MQ=255)

                                                        ttttAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTc                                                                           >  2:272394/1‑140 (MQ=255)

                                                            aaaCTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTg                                                                       <  2:854399/140‑1 (MQ=255)

                                                            aaaCTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTg                                                                       <  1:1520571/140‑1 (MQ=255)

                                                            aaaCTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTg                                                                       <  2:1154342/140‑1 (MQ=255)

                                                                  cTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAac                                                                 >  1:1527027/1‑140 (MQ=255)

                                                                  cTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAac                                                                 >  1:396547/1‑140 (MQ=255)

                                                                  cTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAac                                                                 >  2:181814/1‑140 (MQ=255)

                                                                    tAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAacaa                                                               <  1:660846/140‑1 (MQ=255)

                                                                     aaaaTGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAacaat                                                              <  1:181186/140‑1 (MQ=255)

                                                                      aaaTGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAacaata                                                             <  1:181814/140‑1 (MQ=255)

                                                                      aaaTGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAacaata                                                             <  2:284712/140‑1 (MQ=255)

                                                                        aTGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAacaataca                                                           >  1:1228666/1‑140 (MQ=255)

                                                                        aTGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAacaataca                                                           <  1:180554/140‑1 (MQ=255)

                                                                            ttGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGaaa                                                                    >  2:825078/1‑127 (MQ=255)

                                                                            ttGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGaaa                                                                    <  1:825078/127‑1 (MQ=255)

                                                                                    cATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCt                                               <  2:396547/140‑1 (MQ=255)

                                                                                    cATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCt                                               >  2:609471/1‑140 (MQ=255)

                                                                                     aTTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCtt                                              >  2:1472249/1‑140 (MQ=255)

                                                                                     aTTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCtt                                              >  1:150098/1‑140 (MQ=255)

                                                                                     aTTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCtt                                              >  2:354064/1‑140 (MQ=255)

                                                                                        aaaaaaGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTggg                                           <  2:1527027/140‑1 (MQ=255)

                                                                                        aaaaaaGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTggg                                           <  2:1142953/140‑1 (MQ=255)

                                                                                         aaaaaGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGggt                                          <  2:1228666/140‑1 (MQ=255)

                                                                                               tAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGggtggtggt                                    >  2:1655658/1‑140 (MQ=255)

                                                                                               tAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGggtggtggt                                    >  1:612370/1‑140 (MQ=255)

                                                                                                  gATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAg                                 >  1:1234082/1‑140 (MQ=255)

                                                                                                  gATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAg                                 <  2:593613/140‑1 (MQ=255)

                                                                                                  gATTTATCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAg                                 <  1:844223/140‑1 (MQ=255)

                                                                                                       aTCGATAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGtt                            >  2:5160/1‑140 (MQ=255)

                                                                                                            tAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATg                       <  1:354064/140‑1 (MQ=255)

                                                                                                            tAAAGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATg                       <  1:1472249/140‑1 (MQ=255)

                                                                                                              aaGTAAGCAAGTGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCt                     >  1:378491/1‑140 (MQ=255)

                                                                                                                         tGCAAAGGACTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTAt          >  2:1270204/1‑140 (MQ=255)

                                                                                                                                 aCTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTc  <  2:1234082/140‑1 (MQ=255)

                                                                                                                                 aCTTGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTc  <  2:150098/140‑1 (MQ=255)

ACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTCGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTC  >  NC_000913/1571989‑1572257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A