Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A37 F60 I1 R1 21 79.1 2697330 98.7% 2662264 138.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,452,943 C→T A234T (GCG→ACG)  paaZ ← oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,452,9430CT100.0% 202.7 / NA 58A234T (GCG→ACG) paaZoxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/30);  total (28/30)

AAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGCTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTC  >  NC_000913/1452806‑1453076
                                                                                                                                         |                                                                                                                                     
aaaCAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTg                                                                                                                                     >  1:778684/1‑140 (MQ=255)
 aaCAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGa                                                                                                                                    >  2:976469/1‑140 (MQ=255)
  aCAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGAc                                                                                                                                   <  2:142001/140‑1 (MQ=255)
      cgcAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCg                                                                                                                               >  1:849689/1‑140 (MQ=255)
      cgcAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCg                                                                                                                               >  2:144422/1‑140 (MQ=255)
      cgcAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCg                                                                                                                               >  2:90832/1‑140 (MQ=255)
      cgcAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCg                                                                                                                               >  1:547548/1‑140 (MQ=255)
      cgcAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCg                                                                                                                               >  2:106464/1‑140 (MQ=255)
        cAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTg                                                                                                                             <  2:849689/140‑1 (MQ=255)
        cAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTg                                                                                                                             >  1:987898/1‑140 (MQ=255)
        cAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTg                                                                                                                             >  2:951875/1‑140 (MQ=255)
              ccGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTc                                                                                                                       >  2:1176568/1‑140 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGATGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  1:669064/140‑1 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  2:625663/140‑1 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     >  1:98666/1‑140 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  2:829519/140‑1 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  1:174402/140‑1 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  1:155504/140‑1 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  1:1007542/140‑1 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  1:1248062/140‑1 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  1:1189284/140‑1 (MQ=255)
                ggTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTcac                                                                                                                     <  2:778684/140‑1 (MQ=255)
                          gggTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTTGCAGCATCTGTCCGGTCGCCGTTGACCCCCTCAAAGTCACCACATCCTgg                                                                                                           <  2:1009174/140‑1 (MQ=255)
                          gggTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTgg                                                                                                           <  1:144422/140‑1 (MQ=255)
                            gTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTGACCACATCCTGGCt                                                                                                         <  1:557449/140‑1 (MQ=255)
                                cATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTcc                                                                                                     >  2:711128/1‑140 (MQ=255)
                                     tCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAgatg                                                                                                >  2:900804/1‑140 (MQ=255)
                                         ccAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATcc                                                                                            <  1:951875/140‑1 (MQ=255)
                                                   gcagTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGc                                                                                  <  1:310350/140‑1 (MQ=255)
                                                      gTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAg                                                                               <  1:715322/140‑1 (MQ=255)
                                                       ttCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGc                                                                              >  1:1048512/1‑140 (MQ=255)
                                                       ttCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGc                                                                              >  1:456500/1‑140 (MQ=255)
                                                         cAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCAc                                                                            <  1:15142/140‑1 (MQ=255)
                                                             gAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTAcc                                                                        <  2:88896/140‑1 (MQ=255)
                                                                 cAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAg                                                                    >  2:1347697/1‑140 (MQ=255)
                                                                 cAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAg                                                                    >  1:1111226/1‑140 (MQ=255)
                                                                   gCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGAt                                                                  <  2:456500/140‑1 (MQ=255)
                                                                   gCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGAt                                                                  <  1:106464/140‑1 (MQ=255)
                                                                                 ggggATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTgcgc                                                    >  2:305463/1‑140 (MQ=255)
                                                                                  gggATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGcc                                                   >  1:259382/1‑140 (MQ=255)
                                                                                       aGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCgg                                              >  1:1189982/1‑140 (MQ=255)
                                                                                       aGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCg                                               <  1:53495/139‑1 (MQ=255)
                                                                                       aGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCg                                               >  2:53495/1‑139 (MQ=255)
                                                                                            tGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACa                                         >  2:574265/1‑140 (MQ=255)
                                                                                                gacgaTATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGAc                                     <  1:415094/140‑1 (MQ=255)
                                                                                                  cgaTATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCa                                   <  2:1189982/140‑1 (MQ=255)
                                                                                                  cgaTATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCa                                   <  1:711128/140‑1 (MQ=255)
                                                                                                    atatTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACt                                 <  2:987898/140‑1 (MQ=255)
                                                                                                         tGGCTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGa                            <  1:900804/140‑1 (MQ=255)
                                                                                                            cTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATAAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGa                            <  2:263702/137‑1 (MQ=255)
                                                                                                            cTGAACTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATAAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGa                            >  1:263702/1‑137 (MQ=255)
                                                                                                               aaCTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCa                                   <  2:374550/127‑1 (MQ=255)
                                                                                                               aaCTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCa                                   >  1:374550/1‑127 (MQ=255)
                                                                                                               aaCTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTg                      >  2:292801/1‑140 (MQ=255)
                                                                                                                 cTCGCAGCATCTGTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGAt                    >  1:883716/1‑140 (MQ=255)
                                                                                                                             gTCCGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGcc        <  2:1111226/140‑1 (MQ=255)
                                                                                                                                cGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGa     >  1:896393/1‑140 (MQ=255)
                                                                                                                                cGGTCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGa     <  1:1347697/140‑1 (MQ=255)
                                                                                                                                   tCGCCGTTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTc  >  1:656173/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                     
AAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGCTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTC  >  NC_000913/1452806‑1453076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A