Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F60 I1 R1	24	41.1	1397072	98.8%	1380307	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,465,273	A→G	intergenic (‑43/‑119)	ynbG ← / → ydbA	uncharacterized protein YnbG/putative outer membrane protein, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,465,273	0	A	G	100.0%	171.3 / NA	44	intergenic (‑43/‑119)	ynbG/ydbA	uncharacterized protein YnbG/putative outer membrane protein, N‑terminal fragment
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/18); total (26/18)

TCATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGAAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGATTTATTATGCAAAGGAAA  >  NC_000913/1465141‑1465403

tCATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATg                                                                                                                             <  1:80840/140‑1 (MQ=255)

 cATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGt                                                                                                                            >  1:12057/1‑140 (MQ=255)

 cATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGt                                                                                                                            >  2:696018/1‑140 (MQ=255)

   tACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGttt                                                                                                                          >  2:247585/1‑140 (MQ=255)

    aCCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTg                                                                                                                         >  1:30096/1‑140 (MQ=255)

      cGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGAt                                                                                                                       <  2:657649/140‑1 (MQ=255)

      cGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGAt                                                                                                                       <  2:30096/140‑1 (MQ=255)

        ttttttCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTa                                                                                                                     >  1:685431/1‑140 (MQ=255)

                   tataATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCt                                                                                                          <  1:247585/140‑1 (MQ=255)

                           tttatAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCaa                                                                                                  >  1:30981/1‑140 (MQ=255)

                                  tGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGa                                                                                           <  1:696018/140‑1 (MQ=255)

                                   gagaATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGAt                                                                                          <  2:30981/140‑1 (MQ=255)

                                   gagaATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGAt                                                                                          <  1:173258/140‑1 (MQ=255)

                                    agaATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATg                                                                                         >  2:458137/1‑140 (MQ=255)

                                                     tGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAAt                                                                        <  2:12057/140‑1 (MQ=255)

                                                      gTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATa                                                                       >  1:376183/1‑140 (MQ=255)

                                                       tAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATaa                                                                      >  2:117067/1‑140 (MQ=255)

                                                                     taCACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGt                                                        >  2:608363/1‑140 (MQ=255)

                                                                        acaATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTgg                                                              >  1:171188/1‑131 (MQ=255)

                                                                        acaATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTgg                                                              <  2:171188/131‑1 (MQ=255)

                                                                        acaATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAg                                                     >  2:151097/1‑140 (MQ=255)

                                                                        acaATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAg                                                     >  1:379291/1‑140 (MQ=255)

                                                                         caATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGt                                                    >  2:61614/1‑140 (MQ=255)

                                                                          aaTTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTg                                                   >  2:456987/1‑140 (MQ=255)

                                                                            tttATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCa                                                 >  2:59532/1‑140 (MQ=255)

                                                                            tttATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCa                                                 >  2:240645/1‑140 (MQ=255)

                                                                            tttATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCa                                                 >  2:307489/1‑140 (MQ=255)

                                                                             ttATATATTTCATGGTCTTTTTTTATTCACCTGAATAATAATTGTGACTTCTAGGAAAGTAGGTTTGATTAGATAATAATCTACTGGCAATATTAGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGCGCAt                                                >  2:516709/1‑140 (MQ=255)

                                                                                  tatTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGc                                           <  1:684328/140‑1 (MQ=255)

                                                                                    tttCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGc                                           >  1:402998/1‑138 (MQ=255)

                                                                                    tttCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGc                                           <  2:402998/138‑1 (MQ=255)

                                                                                      tCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGctttc                                       >  2:112622/1‑140 (MQ=255)

                                                                                                tttttATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCg                             >  1:81472/1‑140 (MQ=255)

                                                                                                   ttATTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCa                          <  1:307489/140‑1 (MQ=255)

                                                                                                     aTTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGa                        <  1:117067/140‑1 (MQ=255)

                                                                                                     aTTCACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGa                        <  1:458137/140‑1 (MQ=255)

                                                                                                        cACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAgg                     <  2:81472/140‑1 (MQ=255)

                                                                                                        cACCTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAgg                     <  2:422230/140‑1 (MQ=255)

                                                                                                          ccTGAATTATAATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGAt                   >  2:533175/1‑140 (MQ=255)

                                                                                                                   taATTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGATTTATTATGc          >  2:578614/1‑140 (MQ=255)

                                                                                                                    aaTTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGATTTATTATGCa         <  1:444087/140‑1 (MQ=255)

                                                                                                                     aTTGTGAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGATTTATTATGcct        >  2:414148/1‑138 (MQ=255)

                                                                                                                          gAATTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGATTTATTATGCAAAGGaa   <  1:151097/140‑1 (MQ=255)

                                                                                                                           aaTTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGATTTATTATGCAAAGGaaa  >  1:30417/1‑140 (MQ=255)

                                                                                                                           aaTTATAGGGAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGATTTATTATGCAAAGGaaa  >  1:648481/1‑140 (MQ=255)

TCATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAATTTATATTATATACACAATTTATATATTTCATGGTCTTTTTTTATTCACCTGAATTATAATTGTGAATTATAGGAAAGTATGTTTGATTAGATAATAATCTACTGGCAATATTGGATGTCTTCTATGTTTTAAATAACTAATTGGTCGGGTTAGTGCATCCGGCTTTCTTTATATTCGCCAGAAGGATTTATTATGCAAAGGAAA  >  NC_000913/1465141‑1465403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A