Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A39 F60 I1 R1	17	70.9	2401242	98.9%	2374828	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,018,976	(A)_8→7	intergenic (+39/+37)	matP → / ← ompA	macrodomain Ter protein/outer membrane porin A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,018,969	0	A	.	100.0%	146.5 / NA	35	intergenic (+32/+44)	matP/ompA	macrodomain Ter protein/outer membrane porin A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (19/16); total (19/16)

TCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGC  >  NC_000913/1018830‑1019102

tCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCa                                                                                                                                       >  1:608705/1‑140 (MQ=255)

         aTTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaaccc                                                                                                                              <  1:375683/140‑4 (MQ=255)

            gTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCgc                                                                                                                          <  1:452584/140‑1 (MQ=255)

                  cTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCagcagc                                                                                                                    >  2:244119/1‑140 (MQ=255)

                               cGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTAc                                                                                                       >  2:1031156/1‑140 (MQ=255)

                                      cAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACga                                                                                                <  2:608705/140‑1 (MQ=255)

                                      cAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACga                                                                                                <  1:777697/140‑1 (MQ=255)

                                          gagaAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCa                                                                                                     >  2:525896/1‑131 (MQ=255)

                                          gagaAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCa                                                                                                     <  1:525896/131‑1 (MQ=255)

                                                tACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGc                                                                                      >  2:731039/1‑140 (MQ=255)

                                                     gAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCg                                                                                 >  1:1120528/1‑140 (MQ=255)

                                                      aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCgg                                                                                <  1:680864/140‑1 (MQ=255)

                                                                cttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAGCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACa                                                                      <  2:472695/140‑1 (MQ=255)

                                                                cttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACa                                                                      <  1:1011238/140‑1 (MQ=255)

                                                                 ttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACaa                                                                     >  1:983689/1‑140 (MQ=255)

                                                                           cAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGAt                                                           <  1:1031156/140‑1 (MQ=255)

                                                                              gATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGCTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAcc                                                        >  1:609020/1‑140 (MQ=255)

                                                                                    cAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAAc                                                  >  2:1064988/1‑140 (MQ=255)

                                                                                     aGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACt                                                 <  1:731039/140‑1 (MQ=255)

                                                                                        cATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCg                                              >  2:906579/1‑140 (MQ=255)

                                                                                         aTTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGa                                             >  1:588783/1‑140 (MQ=255)

                                                                                         aTTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGa                                             >  2:675940/1‑140 (MQ=255)

                                                                                          ttGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt                                            >  2:1187990/1‑140 (MQ=255)

                                                                                          ttGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt                                            <  2:356349/140‑1 (MQ=255)

                                                                                          ttGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt                                            <  1:1187990/140‑1 (MQ=255)

                                                                                                     ggAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt                                            >  1:509429/1‑129 (MQ=255)

                                                                                                     ggAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt                                            <  2:509429/129‑1 (MQ=255)

                                                                                                            cTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCgg                          >  2:584285/1‑140 (MQ=255)

                                                                                                                 gAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCa                     >  2:562632/1‑140 (MQ=255)

                                                                                                                 gAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCa                     <  1:1064988/140‑1 (MQ=255)

                                                                                                                    aGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGc                  >  2:301528/1‑140 (MQ=255)

                                                                                                                      tCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAg                <  1:301528/140‑1 (MQ=255)

                                                                                                                         gTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCg             >  2:293658/1‑140 (MQ=255)

                                                                                                                          ttCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAg                >  1:601400/1‑136 (MQ=255)

                                                                                                                          ttCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAg                <  2:601400/136‑1 (MQ=255)

                                                                                                                             aaTTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCa         <  1:244119/140‑1 (MQ=255)

                                                                                                                                    tAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgcagc  >  2:802618/1‑140 (MQ=255)

TCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGC  >  NC_000913/1018830‑1019102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A