Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F60 I1 R1 17 54.2 1827820 99.0% 1809541 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,018,977 C→A intergenic (+40/+36) matP → / ← ompA macrodomain Ter protein/outer membrane porin A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,018,9770CA100.0% 137.2 / NA 43intergenic (+40/+36)matP/ompAmacrodomain Ter protein/outer membrane porin A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/25);  total (18/25)

CTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTG  >  NC_000913/1018848‑1019108
                                                                                                                                  |                                                                                                                                   
cTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGcagcag                                                                                                                           <  2:908385/140‑1 (MQ=255)
    ttGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCggg                                                                                                                       <  2:612010/140‑1 (MQ=255)
         gATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCTGCGGGGtttt                                                                                                                  <  2:869881/140‑1 (MQ=255)
           tGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTc                                                                                                                <  2:802974/140‑1 (MQ=255)
             cGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTa                                                                                                              >  2:431243/1‑140 (MQ=255)
             cGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTa                                                                                                              >  2:181969/1‑140 (MQ=255)
                   aCAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGAc                                                                                                        <  2:896002/140‑1 (MQ=255)
                     aaaGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACga                                                                                                      <  2:764737/140‑1 (MQ=255)
                     aaaGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACga                                                                                                      <  2:598733/140‑1 (MQ=255)
                       agagAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACgaga                                                                                                    >  1:231320/1‑140 (MQ=255)
                       agagAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACgaga                                                                                                    >  2:532270/1‑140 (MQ=255)
                           aaaTACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACtt                                                                                                <  1:611728/140‑1 (MQ=255)
                                     aTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACAAGACGAGAACTTAAGCCTGCgg                                                                                      <  2:120657/140‑1 (MQ=255)
                                        aaaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTg                                                                                   >  2:463796/1‑140 (MQ=255)
                                                 ttctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAGCTTAAGCCTGCGGCTGAGTTACAAc                                                                          <  2:380799/139‑1 (MQ=255)
                                                 ctctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAAc                                                                          <  1:431243/140‑1 (MQ=255)
                                                   ctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGt                                                                        >  1:269937/1‑140 (MQ=255)
                                                       aGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCttt                                                                    >  1:592670/1‑140 (MQ=255)
                                                          aGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGAt                                                                 <  2:723376/140‑1 (MQ=255)
                                                          aGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGAt                                                                 <  2:275287/140‑1 (MQ=255)
                                                            gATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAc                                                               >  2:627865/1‑140 (MQ=255)
                                                            gATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAc                                                               >  1:307698/1‑140 (MQ=255)
                                                                tGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCttt                                                           >  1:178776/1‑140 (MQ=255)
                                                                      cATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTc                                                     >  2:317162/1‑140 (MQ=255)
                                                                               gTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGCTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAc                                            >  2:521377/1‑140 (MQ=255)
                                                                                   ggAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGcga                                        <  2:592670/140‑1 (MQ=255)
                                                                                   ggAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGcga                                        <  1:181969/140‑1 (MQ=255)
                                                                                   ggAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGcga                                        >  2:335580/1‑140 (MQ=255)
                                                                                             aCGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAg                              >  1:682192/1‑140 (MQ=255)
                                                                                             aCGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAg                              >  1:610832/1‑140 (MQ=255)
                                                                                              cGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGc                             >  2:555284/1‑140 (MQ=255)
                                                                                                  aGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAgg                         <  2:231320/140‑1 (MQ=255)
                                                                                                  aGTCAGTTCAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAgg                         <  2:178776/140‑1 (MQ=255)
                                                                                                         tCAATTTACTAAAGGCAAAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCg                   <  1:216925/140‑1 (MQ=255)
                                                                                                          cAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGTAGTCGAt                 <  2:610832/140‑1 (MQ=255)
                                                                                                          cAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGAt                 <  2:273525/140‑1 (MQ=255)
                                                                                                          cAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGAt                 <  1:555284/140‑1 (MQ=255)
                                                                                                          cAATTTACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTAATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGAt                 <  1:686518/140‑1 (MQ=255)
                                                                                                              ttACTAAAGGC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGt             <  1:541063/140‑1 (MQ=255)
                                                                                                                      ggC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACg     >  2:295500/1‑140 (MQ=255)
                                                                                                                      ggC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACg     <  1:521377/140‑1 (MQ=255)
                                                                                                                       gC‑AAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGc    >  1:891137/1‑140 (MQ=255)
                                                                                                                          aaaaaaaaACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTg  <  1:463796/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
CTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTG  >  NC_000913/1018848‑1019108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A