Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F60 I1 R1	24	41.1	1397072	98.8%	1380307	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,211	T→G	intergenic (‑79/‑34)	ychJ ← / → rssA	NTF2‑like domain‑containing protein YchJ/putative patatin‑like phospholipase RssA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,211	0	T	G	100.0%	147.1 / NA	39	intergenic (‑79/‑34)	ychJ/rssA	NTF2‑like domain‑containing protein YchJ/putative patatin‑like phospholipase RssA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (23/16); total (23/16)

GGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAA  >  NC_000913/1289078‑1289343

ggTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGAtt                                                                                                                                >  2:610439/1‑140 (MQ=255)

 gTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTg                                                                                                                               <  1:42657/140‑1 (MQ=255)

   ggCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCa                                                                                                                             >  2:408356/1‑140 (MQ=255)

     cAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCAtt                                                                                                                           >  2:570588/1‑140 (MQ=255)

                  aCTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCtt                                                                                                              >  2:404500/1‑140 (MQ=255)

                             cTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGaataat                                                                                                   >  1:232642/1‑140 (MQ=255)

                              tACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATg                                                                                                  >  2:486112/1‑140 (MQ=255)

                                 cacaGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATgaga                                                                                               <  2:232642/140‑1 (MQ=255)

                                     gggACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGa                                                                                           >  1:320751/1‑140 (MQ=255)

                                              cTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGCGCCGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAg                                                                                  >  1:65973/1‑140 (MQ=255)

                                                 agaCACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGc                                                                               <  1:610439/140‑1 (MQ=255)

                                                   acacAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTg                                                                             <  1:408356/140‑1 (MQ=255)

                                                   acacAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTg                                                                             <  2:250027/140‑1 (MQ=255)

                                                      caAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGgcg                                                                          <  1:685955/140‑1 (MQ=255)

                                                       aaaTAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGgcgc                                                                         >  2:159008/1‑140 (MQ=255)

                                                                 ccTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGc                                                                               <  2:670224/124‑1 (MQ=255)

                                                                 ccTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGc                                                                               >  1:670224/1‑124 (MQ=255)

                                                                               cGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGtt                                                 >  1:547510/1‑140 (MQ=255)

                                                                                    tATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCg                                            >  2:250474/1‑140 (MQ=255)

                                                                                    tATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCg                                            >  2:553865/1‑140 (MQ=255)

                                                                                          ccGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATAtt                                      <  2:547510/140‑1 (MQ=255)

                                                                                            gCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg                                    <  1:662361/140‑1 (MQ=255)

                                                                                            gCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg                                    <  2:497305/140‑1 (MQ=255)

                                                                                            gCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg                                    >  1:497305/1‑140 (MQ=255)

                                                                                                 aGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGtta                               >  1:486542/1‑140 (MQ=255)

                                                                                                         tATGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgcg                       <  2:486542/140‑1 (MQ=255)

                                                                                                           tGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATAtt                                      <  2:643221/123‑1 (MQ=255)

                                                                                                           tGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATAtt                                      >  1:643221/1‑123 (MQ=255)

                                                                                                           tGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgcgc                      >  2:391910/1‑139 (MQ=255)

                                                                                                           tGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATgcgc                      <  1:391910/139‑1 (MQ=255)

                                                                                                           tGTTAACCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCt                     >  1:34955/1‑140 (MQ=255)

                                                                                                                aCCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGTATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaa                >  2:517116/1‑140 (MQ=255)

                                                                                                                aCCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTa                    >  2:24076/1‑136 (MQ=255)

                                                                                                                aCCGAGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTa                    <  1:24076/136‑1 (MQ=255)

                                                                                                                    aGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGt            <  1:404500/140‑1 (MQ=255)

                                                                                                                    aGCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGt            <  1:159008/140‑1 (MQ=255)

                                                                                                                     gCAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTg           >  2:666573/1‑140 (MQ=255)

                                                                                                                      cAGTAGCGATGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTgg          >  1:473788/1‑140 (MQ=255)

                                                                                                                           gCGATGTGGCGACGATTGCATTCTAGGGGAATCTTGCGGGAATAATGAGATAGATAAAAATAGGGCCGGCGCTGGGTTCTGGTGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCTCGAAAAAAAGTgggaagt     >  2:689196/1‑136 (MQ=255)

                                                                                                                              aTGTGGCGACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGaa  >  2:323198/1‑140 (MQ=255)

GGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAA  >  NC_000913/1289078‑1289343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A