Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F60 I1 R1	15	71.5	2434152	98.6%	2400073	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,147,333	A→T	intergenic (+18/‑34)	yceD → / → rpmF	DUF177 domain‑containing protein YceD/50S ribosomal subunit protein L32

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,333	0	A	T	100.0%	172.8 / NA	50	intergenic (+18/‑34)	yceD/rpmF	DUF177 domain‑containing protein YceD/50S ribosomal subunit protein L32
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (23/27); total (23/27)

GATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTT  >  NC_000913/1147205‑1147466

gATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATaaa                                                                                                                            >  1:1088311/1‑140 (MQ=255)

gATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATaaa                                                                                                                            >  1:943699/1‑140 (MQ=255)

 aTTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAAc                                                                                                                           <  1:407757/140‑1 (MQ=255)

     tGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCg                                                                                                                         <  2:748347/138‑1 (MQ=255)

     tGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCg                                                                                                                         >  1:748347/1‑138 (MQ=255)

     tGAACACTGCGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCTAGCTTAAAGCGTAAGTAATTGGAGCTCCCCGTTGCTTCGGGGATATACCGTa                                                                                                                       >  2:146543/1‑140 (MQ=255)

           cTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGTACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTgag                                                                                                                 <  2:164972/140‑1 (MQ=255)

           cTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTgag                                                                                                                 <  1:146543/140‑1 (MQ=255)

            tgtgAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGa                                                                                                                                <  1:574894/124‑1 (MQ=255)

            tgtgAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGa                                                                                                                                >  2:574894/1‑124 (MQ=255)

            tgtgAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATa                                                                                                                              >  1:586301/1‑126 (MQ=255)

            tgtgAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATa                                                                                                                              <  2:586301/126‑1 (MQ=255)

            tgtgAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTgagg                                                                                                                >  1:234424/1‑140 (MQ=255)

                aaGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTa                                                                                                            <  2:514528/140‑1 (MQ=255)

                aaGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTa                                                                                                            <  2:298392/140‑1 (MQ=255)

                 aGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTaa                                                                                                           >  1:391514/1‑140 (MQ=255)

                      ccGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTgag                                                                                                                 <  2:991792/129‑1 (MQ=255)

                      ccGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTgag                                                                                                                 >  1:991792/1‑129 (MQ=255)

                             ggACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCg                                                                                               <  2:182181/140‑1 (MQ=255)

                              gACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGt                                                                                              >  1:1177675/1‑140 (MQ=255)

                               aCATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTa                                                                                             <  2:943699/140‑1 (MQ=255)

                                  tGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTacaa                                                                                          <  2:291822/140‑1 (MQ=255)

                                     tCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAg                                                                                       <  1:338748/140‑1 (MQ=255)

                                      cTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGa                                                                                      <  2:1088311/140‑1 (MQ=255)

                                         tGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATa                                                                                   <  1:995074/140‑1 (MQ=255)

                                           gTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGTCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATaaa                                                                                 >  1:807849/1‑140 (MQ=255)

                                               aCTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGa                                                                                      >  2:189253/1‑131 (MQ=255)

                                               aCTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGa                                                                                      <  1:189253/131‑1 (MQ=255)

                                               aCTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAaaccaa                                                                             >  2:593069/1‑140 (MQ=255)

                                                   ccTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCg                                                                         >  2:404889/1‑140 (MQ=255)

                                                   ccTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCg                                                                         >  2:612405/1‑140 (MQ=255)

                                                             cgcAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGt                                                               >  1:817317/1‑140 (MQ=255)

                                                             cgcAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGt                                                               >  2:937554/1‑140 (MQ=255)

                                                             cgcAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGt                                                               >  1:565400/1‑140 (MQ=255)

                                                                 aaaGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCa                                                           <  1:952317/140‑1 (MQ=255)

                                                                   aGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATg                                                         <  2:1177675/140‑1 (MQ=255)

                                                                      cAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGcgt                                                      <  2:565400/140‑1 (MQ=255)

                                                                        aaCCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGcgtcg                                                    <  2:817317/140‑1 (MQ=255)

                                                                                         aGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGa                                   <  2:391514/140‑1 (MQ=255)

                                                                                               cTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAg                             >  2:781745/1‑140 (MQ=255)

                                                                                                 tAAAGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTc                           >  2:564236/1‑140 (MQ=255)

                                                                                                    aGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGc                               >  1:913/1‑133 (MQ=255)

                                                                                                    aGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGc                               >  1:82716/1‑133 (MQ=255)

                                                                                                    aGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGc                               <  2:82716/133‑1 (MQ=255)

                                                                                                    aGCGTAAGTAATTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGc                               <  2:913/133‑1 (MQ=255)

                                                                                                        taagtaaTTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGcc                    >  1:1190033/1‑140 (MQ=255)

                                                                                                         aagtaaTTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCct                   >  2:1130255/1‑140 (MQ=255)

                                                                                                           gtaaTTGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCctgt                 <  2:782176/140‑1 (MQ=255)

                                                                                                                tGGTGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTa            <  1:612405/140‑1 (MQ=255)

                                                                                                                   tGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGAc         >  2:1203954/1‑140 (MQ=255)

                                                                                                                   tGCTCCCCGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGAc         <  1:1130255/140‑1 (MQ=255)

                                                                                                                          cGTTGGTTCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACtt  <  1:781745/140‑1 (MQ=255)

GATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTT  >  NC_000913/1147205‑1147466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A