Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F60 I1 R1
|
17 |
33.2 |
1123130 |
98.8% |
1109652 |
138.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,622,354 |
G→T |
E196* (GAA→TAA) |
yjjJ → |
toxin YjjJ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,622,354 | 0 | G | T | 100.0%
| 73.7
/ NA
| 22 | E196* (GAA→TAA) | yjjJ | toxin YjjJ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (11/11); total (11/11) |
TGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGAC > NC_000913/4622221‑4622488
|
tGAATATACTGGCGGTTGGTTTGGGGGGGGGGGGAATTATCAGCGAGGGGTTTCTGCACAACACCCAGCGGGAATTCCTTTGGGTCAAAAACTCACCCAATTCCAACAGCCGGCCATTGATGCACTGGCAGGATAAAttt > 2:122173/1‑139 (MQ=255)
gggaATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATC‑AAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCaaaa < 2:202495/140‑1 (MQ=255)
cacaACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCg > 2:401356/1‑140 (MQ=255)
acaacaCCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACcta < 2:311804/123‑1 (MQ=255)
acaacaCCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACcta > 1:311804/1‑123 (MQ=255)
caacaCCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTc < 1:274800/140‑1 (MQ=255)
ccTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCaa > 1:217684/1‑140 (MQ=255)
ccTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCaa < 1:357464/140‑1 (MQ=255)
tGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATa > 1:505070/1‑140 (MQ=255)
tGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATa > 2:223176/1‑140 (MQ=255)
tGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGACGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCa < 1:263104/137‑1 (MQ=255)
tGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGACGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCa > 2:263104/1‑137 (MQ=255)
ctctGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTg < 2:217684/140‑1 (MQ=255)
aaaCTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCAc < 1:387853/140‑1 (MQ=255)
aCCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAAtt > 1:102357/1‑131 (MQ=255)
aCCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAAtt < 2:102357/131‑1 (MQ=255)
aCCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTAcc > 2:151806/1‑140 (MQ=255)
aaCAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAAcc > 1:11248/1‑140 (MQ=255)
cAGCTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACcgc > 1:265917/1‑140 (MQ=255)
cTGGCAAGTGATGCACTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGt > 2:374774/1‑140 (MQ=255)
aCTGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTggg < 1:151806/140‑1 (MQ=255)
tGGCAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCTAACGTTGGGGt < 2:265917/140‑1 (MQ=255)
cAGGATAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTTAGCCAACGTTGGGGTGAc < 1:374774/140‑1 (MQ=255)
|
TGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGAC > NC_000913/4622221‑4622488
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
