Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F60 I1 R1	21	41.4	1402642	98.7%	1384407	138.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,234,056	T→A	E221V (GAA→GTA)	nhaB ←	Na(+):H(+) antiporter NhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,234,056	0	T	A	100.0%	96.1 / NA	29	E221V (GAA→GTA)	nhaB	Na(+):H(+) antiporter NhaB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (13/16); total (13/16)

ACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGT  >  NC_000913/1233922‑1234192

aCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGccc                                                                                                                                     >  2:266994/1‑140 (MQ=255)

    aCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCccacca                                                                                                                                 >  2:62398/1‑140 (MQ=255)

      gCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATg                                                                                                                               >  1:302144/1‑140 (MQ=255)

          cTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCa                                                                                                                           >  1:322494/1‑140 (MQ=255)

               ctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTAcg                                                                                                                      >  1:169954/1‑140 (MQ=255)

               ctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTAcg                                                                                                                      >  1:556427/1‑140 (MQ=255)

               ctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACAGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTAcg                                                                                                                      <  2:137960/140‑1 (MQ=255)

                    agcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACgccgcc                                                                                                                 >  2:320723/1‑140 (MQ=255)

                    agcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACgccgcc                                                                                                                 <  1:62398/140‑1 (MQ=255)

                        ggcaggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAAt                                                                                                             >  2:177037/1‑140 (MQ=255)

                                  cAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGa                                                                                                   <  2:673410/140‑1 (MQ=255)

                                    ggCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGaca                                                                                                 <  2:556427/140‑1 (MQ=255)

                                    ggCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGaca                                                                                                 <  2:470195/140‑1 (MQ=255)

                                    ggCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGaca                                                                                                 <  2:324761/140‑1 (MQ=255)

                                        acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCgg                                                                                             <  2:679714/140‑1 (MQ=255)

                                        acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCgg                                                                                             <  1:180647/140‑1 (MQ=255)

                                                                cGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGaa                                                                     <  1:115197/140‑1 (MQ=255)

                                                                                   aaaTCGCCAAAATGCCAGCCAGCCGCTTTAGTGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAg                                                  <  1:528795/140‑1 (MQ=255)

                                                                                    aaTCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGa                                                 >  2:459477/1‑140 (MQ=255)

                                                                                       cGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAcc                                              <  2:322494/140‑1 (MQ=255)

                                                                                        gCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCa                                             <  2:302144/140‑1 (MQ=255)

                                                                                                    gccagccGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCt                                 >  1:446094/1‑140 (MQ=255)

                                                                                                         ccGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCa                                             >  2:120482/1‑123 (MQ=255)

                                                                                                         ccGCTTTAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCa                                             <  1:120482/123‑1 (MQ=255)

                                                                                                               tAGCGATGATCAGGTTCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGa                      <  1:177037/140‑1 (MQ=255)

                                                                                                                              tCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGa                      <  1:402377/125‑1 (MQ=255)

                                                                                                                              tCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGa                      >  2:402377/1‑125 (MQ=255)

                                                                                                                              tCTGTGGTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAgg       >  1:295966/1‑140 (MQ=255)

                                                                                                                                   ggTACGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGgt  <  2:295966/140‑1 (MQ=255)

ACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGT  >  NC_000913/1233922‑1234192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A