Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A28 F60 I1 R1	17	39.6	1345070	98.9%	1330274	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,417,918	C→G	G31R (GGG→CGG)	ydaE ←	Rac prophage; zinc‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,417,918	0	C	G	100.0%	196.4 / NA	51	G31R (GGG→CGG)	ydaE	Rac prophage; zinc‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (23/28); total (23/28)

TTGAGCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAA  >  NC_000913/1417784‑1418052

ttGAGCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCa                                                                                                                                   <  2:350563/140‑1 (MQ=255)

ttGAGCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCa                                                                                                                                   <  2:493015/140‑1 (MQ=255)

   aGCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATTTGGCCTTTTTCGGCACAAGTTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCAtga                                                                                                                                >  1:661340/1‑140 (MQ=255)

   aGCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCAtga                                                                                                                                >  2:237997/1‑140 (MQ=255)

   aGCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCAtga                                                                                                                                >  2:594503/1‑140 (MQ=255)

    gCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCAtgaa                                                                                                                               >  1:133613/1‑140 (MQ=255)

    gCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCAtgaa                                                                                                                               <  2:235420/140‑1 (MQ=255)

        aTACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCAtgaagtga                                                                                                                           <  1:321298/140‑1 (MQ=255)

         tACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCAtgaagtgaa                                                                                                                          >  2:211000/1‑140 (MQ=255)

           ccTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGc                                                                                                                        <  1:211000/140‑1 (MQ=255)

             tGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCAt                                                                                                                      >  1:320143/1‑140 (MQ=255)

              gCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGc                                                                                                                        <  1:471320/137‑1 (MQ=255)

              gCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGc                                                                                                                        >  2:471320/1‑137 (MQ=255)

              gCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCAtt                                                                                                                     <  1:665761/140‑1 (MQ=255)

              gCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCAtt                                                                                                                     <  1:237965/140‑1 (MQ=255)

              gCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCAtt                                                                                                                     <  1:207451/140‑1 (MQ=255)

               ccATTTCTGGCATAAAATCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTc                                                                                                                    >  1:644406/1‑140 (MQ=255)

                      tGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAAt                                                                                                             >  2:655162/1‑140 (MQ=255)

                          aTAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAgct                                                                                                         <  1:655162/140‑1 (MQ=255)

                          aTAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAgct                                                                                                         <  1:667037/140‑1 (MQ=255)

                           tAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAgctt                                                                                                        >  1:568237/1‑140 (MQ=255)

                               ttCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAgctttgct                                                                                                    <  2:133613/140‑1 (MQ=255)

                                         aaTAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACg                                                                                          >  1:472214/1‑140 (MQ=255)

                                                     attaaAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCaa                                                                              <  2:66723/140‑1 (MQ=255)

                                                     attaaAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCaa                                                                              >  2:87498/1‑140 (MQ=255)

                                                     attaaAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCaa                                                                              <  2:320143/140‑1 (MQ=255)

                                                     attaaAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCaa                                                                              <  2:605800/140‑1 (MQ=255)

                                                        aaaGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAgg                                                                           >  1:624424/1‑140 (MQ=255)

                                                        aaaGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAgg                                                                           >  1:474691/1‑140 (MQ=255)

                                                        aaaGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAgg                                                                           >  1:310131/1‑140 (MQ=255)

                                                               aTGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAg                                                                    >  1:312473/1‑140 (MQ=255)

                                                                    ccATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGcac                                                                 <  2:553014/138‑1 (MQ=255)

                                                                    ccATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGcac                                                                 >  1:553014/1‑138 (MQ=255)

                                                                    ccATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACat                                                               >  1:487859/1‑140 (MQ=255)

                                                                        cTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACatttta                                                           >  2:90257/1‑140 (MQ=255)

                                                                                   cGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGtc                                                <  1:607325/140‑1 (MQ=255)

                                                                                     gCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGtcat                                              >  1:197005/1‑140 (MQ=255)

                                                                                     gCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGtcat                                              <  1:594503/140‑1 (MQ=255)

                                                                                         aaGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCAcc                                          <  2:237677/140‑1 (MQ=255)

                                                                                               cACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACt                                    <  1:87498/140‑1 (MQ=255)

                                                                                                     aaTATTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCt                              >  1:440318/1‑140 (MQ=255)

                                                                                                       tatTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCttt                            <  2:487859/140‑1 (MQ=255)

                                                                                                        atTTTCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTa                           <  1:6908/140‑1 (MQ=255)

                                                                                                         ttttCTCGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTAc                          >  1:38634/1‑140 (MQ=255)

                                                                                                           ttCTCGGTTCGTCTTTTGATAAAATCCCTTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTTCTCATCTTCTTTTATTCAACGGTAGTAAACACATTTTATATTCTTAGTCATCTCCATGTCTCCGCCTTTACAg                        <  2:661340/140‑1 (MQ=255)

                                                                                                             ctcGGTTCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGt                      <  2:568237/140‑1 (MQ=255)

                                                                                                                   tCGTCTTTTGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGAGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCa                >  1:8313/1‑140 (MQ=255)

                                                                                                                         tttGATAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGa          <  2:474691/140‑1 (MQ=255)

                                                                                                                              tAAAATCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAg     <  2:624424/140‑1 (MQ=255)

                                                                                                                                aaaTCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAgg    >  1:264880/1‑139 (MQ=255)

                                                                                                                                aaaTCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAgg    <  2:264880/139‑1 (MQ=255)

                                                                                                                                aaaTCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGa   <  1:237997/140‑1 (MQ=255)

                                                                                                                                 aaTCCGGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGaa  <  2:440318/140‑1 (MQ=255)

TTGAGCGAATACCTGCCATTTCTGGCATAAATTCAGTTTCGAATAGTCAATTAATTAAAGTTCATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAA  >  NC_000913/1417784‑1418052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A