Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A26 F60 I1 R1	17	54.2	1827820	99.0%	1809541	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,879,288	C→T	G278S (GGC→AGC)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,879,288	0	C	T	97.7%	143.3 / ‑4.5	44	G278S (GGC→AGC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (22/21); total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTT  >  NC_000913/3879153‑3879421

cgtccGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCa                                                                                                                                   <  2:153475/137‑1 (MQ=255)

 gACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCAc                                                                                                                                  >  1:758676/1‑140 (MQ=255)

              ccTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAAtgtt                                                                                                                     <  2:368397/140‑1 (MQ=255)

                 tCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATgttgtt                                                                                                                  >  2:710921/1‑140 (MQ=255)

                     gcgcATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTa                                                                                                              >  1:461135/1‑140 (MQ=255)

                                     ggCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGtttt                                                                                              >  2:292571/1‑140 (MQ=255)

                                       cgcTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTcc                                                                                            <  1:124422/140‑1 (MQ=255)

                                       cgcTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTcc                                                                                            >  2:584640/1‑140 (MQ=255)

                                         cTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTg                                                                                          >  2:313947/1‑140 (MQ=255)

                                           gACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGa                                                                                        <  2:617381/140‑1 (MQ=255)

                                              tttGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGACACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGc                                                                                     >  2:168932/1‑140 (MQ=255)

                                                 ggCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGACACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCAt                                                                                  <  1:168932/140‑1 (MQ=255)

                                                      ttttGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTc                                                                             >  2:94214/1‑140 (MQ=255)

                                                      ttttGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTc                                                                             <  1:507588/140‑1 (MQ=255)

                                                        ttGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCa                                                                           <  2:278908/140‑1 (MQ=255)

                                                           cTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTg                                                                        <  2:817659/140‑1 (MQ=255)

                                                           cTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTg                                                                        <  2:27628/140‑1 (MQ=255)

                                                                              tGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCa                                                     >  1:163771/1‑140 (MQ=255)

                                                                                 aGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATa                                                  <  2:79971/140‑1 (MQ=255)

                                                                                 aGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATa                                                  <  2:254599/140‑1 (MQ=255)

                                                                                        cAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCtt                                           >  2:671315/1‑140 (MQ=255)

                                                                                         aGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCttt                                          >  1:680772/1‑140 (MQ=255)

                                                                                          gggTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCtttt                                         >  2:434082/1‑140 (MQ=255)

                                                                                           ggTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCttttt                                        <  1:584640/140‑1 (MQ=255)

                                                                                           ggTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCttttt                                        <  1:290326/140‑1 (MQ=255)

                                                                                             tACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCa                                      >  1:872533/1‑140 (MQ=255)

                                                                                             tACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCa                                      >  1:497409/1‑140 (MQ=255)

                                                                                              gcGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAg                                     <  2:758676/139‑1 (MQ=255)

                                                                                                 ggTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTgg                                  <  1:671315/140‑1 (MQ=255)

                                                                                                 ggTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTgg                                  <  2:306985/140‑1 (MQ=255)

                                                                                                  gTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTTGTCAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATTCCGTCTTTTTCAGTGga                                 <  1:551103/140‑1 (MQ=255)

                                                                                                   tCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgag                                <  1:355485/140‑1 (MQ=255)

                                                                                                   tCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgag                                <  1:292571/140‑1 (MQ=255)

                                                                                                     aTCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGaa                              >  1:249518/1‑140 (MQ=255)

                                                                                                            cACGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTagaag                       >  1:588810/1‑140 (MQ=255)

                                                                                                             aCGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTagaaga                      <  2:461135/140‑1 (MQ=255)

                                                                                                             aCGGAAGCCTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTagaaga                      <  1:710921/140‑1 (MQ=255)

                                                                                                                     cTGCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATTCCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCggg              <  2:258816/140‑1 (MQ=255)

                                                                                                                       gCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTg            >  1:360362/1‑140 (MQ=255)

                                                                                                                       gCCAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTg            >  1:437875/1‑140 (MQ=255)

                                                                                                                         cAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGa          <  1:896536/140‑1 (MQ=255)

                                                                                                                         cAGGTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGa          >  2:823702/1‑140 (MQ=255)

                                                                                                                           ggTGAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATc        >  1:899406/1‑140 (MQ=255)

                                                                                                                              gAGTACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGc     >  2:540747/1‑140 (MQ=255)

                                                                                                                                 tACCGCTGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGtt  >  2:225083/1‑140 (MQ=255)

CGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTT  >  NC_000913/3879153‑3879421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A