Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A33 F60 I1 R1	17	65.5	2241852	98.8%	2214949	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,972,110	T→G	L284R (CTT→CGT)	wecC →	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,972,110	0	T	G	100.0%	174.7 / NA	46	L284R (CTT→CGT)	wecC	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (22/24); total (22/24)

TAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTG  >  NC_000913/3971973‑3972243

tAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTa                                                                                                                                     >  2:359577/1‑140 (MQ=255)

tAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTa                                                                                                                                     >  1:637271/1‑140 (MQ=255)

     tCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGt                                                                                                                                >  1:1066659/1‑140 (MQ=255)

       tGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTAc                                                                                                                              >  2:684908/1‑140 (MQ=255)

        gggAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTAcc                                                                                                                             <  2:1066659/140‑1 (MQ=255)

              tGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACcgcgcgc                                                                                                                       >  2:481637/1‑140 (MQ=255)

               gATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACcgcgcgcg                                                                                                                      <  1:481637/140‑1 (MQ=255)

                      cTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGaa                                                                                                               <  2:776285/140‑1 (MQ=255)

                      cTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAACAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGaa                                                                                                               <  2:14932/140‑1 (MQ=255)

                          cGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGAt                                                                                                           <  1:874396/140‑1 (MQ=255)

                              tCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATcaca                                                                                                       >  2:1117199/1‑140 (MQ=255)

                               cGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACaa                                                                                                      <  2:461334/140‑1 (MQ=255)

                                     ccTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGtt                                                                                                <  1:783651/140‑1 (MQ=255)

                                         gcgTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTgg                                                                                            >  2:962430/1‑140 (MQ=255)

                                                  ttcttcAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGAt                                                                                   <  1:1117199/140‑1 (MQ=255)

                                                   tcttcAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATc                                                                                  >  2:453453/1‑140 (MQ=255)

                                                     ttcAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAg                                                                                >  2:790316/1‑140 (MQ=255)

                                                       cAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGt                                                                              <  1:962430/140‑1 (MQ=255)

                                                           ctggccctggcGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGaaa                                                                          <  1:453453/140‑1 (MQ=255)

                                                              gccctggcGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTa                                                                                        >  2:1029838/1‑123 (MQ=255)

                                                              gccctggcGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTa                                                                                        <  1:1029838/123‑1 (MQ=255)

                                                                   ggcGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGt                                                                  >  1:1104742/1‑140 (MQ=255)

                                                                         ggCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGa                                                            >  1:948535/1‑140 (MQ=255)

                                                                         ggCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGa                                                            >  2:320527/1‑140 (MQ=255)

                                                                         ggCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGGGGGTgg                                                            >  2:263339/1‑139 (MQ=255)

                                                                                aCTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTg                                                     <  1:684908/140‑1 (MQ=255)

                                                                                  tGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTggc                                                   <  2:637271/140‑1 (MQ=255)

                                                                                    cATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTggcgg                                                 <  1:790316/140‑1 (MQ=255)

                                                                                    cATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGCGGGTGGTTGCCTggcgg                                                 >  2:197406/1‑140 (MQ=255)

                                                                                       tGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTa                                              <  1:263339/140‑1 (MQ=255)

                                                                                       tGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTa                                              <  1:320527/140‑1 (MQ=255)

                                                                                        gCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTAc                                             >  1:1096706/1‑140 (MQ=255)

                                                                                            ttGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTggcgg                                                 >  2:684768/1‑132 (MQ=255)

                                                                                            ttGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTggcgg                                                 <  1:684768/132‑1 (MQ=255)

                                                                                               aTCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATaaa                                      <  2:432937/140‑1 (MQ=255)

                                                                                                 ccGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAAcg                                    >  2:530405/1‑140 (MQ=255)

                                                                                                   gTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAAcgcg                                  >  2:253832/1‑140 (MQ=255)

                                                                                                        ttATCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGt                             <  2:1096706/140‑1 (MQ=255)

                                                                                                          aTCGTGGCACAGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGa                           >  1:371698/1‑140 (MQ=255)

                                                                                                                 cacaGAACCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGaaa                    >  1:766919/1‑140 (MQ=255)

                                                                                                                      aaCCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGc               >  1:49619/1‑140 (MQ=255)

                                                                                                                      aaCCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGc               <  1:884665/140‑1 (MQ=255)

                                                                                                                      aaCCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGc               <  2:948535/140‑1 (MQ=255)

                                                                                                                      aaCCCCCAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGc               <  1:197406/140‑1 (MQ=255)

                                                                                                                           ccAGCAGGCGCGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCt          <  1:453575/140‑1 (MQ=255)

                                                                                                                                  gcgcGGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCt   <  2:49619/140‑1 (MQ=255)

                                                                                                                                  gcgcGGCGTAACCGTACCGTGCGCGAAGTGAACGATCACAAGCCGTTCTGGGTTATCGATCAGGTGAAAGCGGTGGTGGCTGATTGCCTGGGGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCt   <  2:971054/140‑1 (MQ=255)

                                                                                                                                   cgcgGCGTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGCTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGGTTGTCTTGCGGCTAACCGTAAACACCCCGGGGAACTGGAAAACGCCTGCTTTTGTCTg  >  2:453575/1‑140 (MQ=255)

TAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTG  >  NC_000913/3971973‑3972243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A