Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	143	68.4	2327250	99.3%	2310959	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,987,060	T→C	V405A (GTA→GCA)	yqeG →	putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,987,060	0	T	C	98.9%	320.1 / ‑5.2	89	V405A (GTA→GCA)	yqeG	putative transporter YqeG
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (45/43); total (45/44)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTA  >  NC_000913/2986928‑2987194

tCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTc                                                                                                                                 >  1:526615/1‑140 (MQ=255)

tCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTc                                                                                                                                 >  1:853581/1‑140 (MQ=255)

tCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTc                                                                                                                                 >  1:143523/1‑140 (MQ=255)

  aGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCtt                                                                                                                               <  2:700412/140‑1 (MQ=255)

     ggCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAg                                                                                                                            <  1:891715/140‑1 (MQ=255)

     ggCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAg                                                                                                                            >  2:582233/1‑140 (MQ=255)

       cccGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCt                                                                                                                          <  2:84665/140‑1 (MQ=255)

          gCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAat                                                                                                                       >  2:1120831/1‑140 (MQ=255)

          gCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAat                                                                                                                       >  2:853331/1‑140 (MQ=255)

          gCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAat                                                                                                                       >  1:748766/1‑140 (MQ=255)

            tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAatat                                                                                                                     <  2:1009427/140‑1 (MQ=255)

            tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAatat                                                                                                                     <  2:20140/140‑1 (MQ=255)

            tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAatat                                                                                                                     <  2:423086/140‑1 (MQ=255)

            tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAatat                                                                                                                     <  2:635283/140‑1 (MQ=255)

            tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAatat                                                                                                                     >  1:788486/1‑140 (MQ=255)

            tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAatat                                                                                                                     <  2:748766/140‑1 (MQ=255)

            tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTCCTTTAGCTAatat                                                                                                                     <  1:630982/140‑1 (MQ=255)

                      gATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGa                                                                                                           <  2:847257/140‑1 (MQ=255)

                      gATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGa                                                                                                           >  2:286423/1‑140 (MQ=255)

                      gATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGa                                                                                                           <  2:493084/140‑1 (MQ=255)

                           ttttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGtt                                                                                                      >  2:635387/1‑140 (MQ=255)

                                atcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCaaaa                                                                                                 >  2:11461/1‑140 (MQ=255)

                                    tGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGc                                                                                             >  1:870378/1‑140 (MQ=255)

                                     gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGa                                                                                                           <  2:241808/125‑1 (MQ=255)

                                     gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGa                                                                                                           >  1:241808/1‑125 (MQ=255)

                                     gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                            >  2:338314/1‑140 (MQ=255)

                                     gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                            >  1:203121/1‑140 (MQ=255)

                                              gTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTg                                                                                   >  1:697779/1‑140 (MQ=255)

                                              gTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTg                                                                                   <  1:1036018/140‑1 (MQ=255)

                                               tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTa                                                                                           >  2:113127/1‑131 (MQ=255)

                                               tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTa                                                                                           <  1:113127/131‑1 (MQ=255)

                                               tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGa                                                                                  >  1:493243/1‑140 (MQ=255)

                                               tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGa                                                                                  >  1:416217/1‑140 (MQ=255)

                                               tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGa                                                                                  >  2:1075148/1‑140 (MQ=255)

                                                     tATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccatt                                                                            <  2:124302/140‑1 (MQ=255)

                                                           aTCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTGATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCAttt                                                                      >  1:824909/1‑140 (MQ=255)

                                                             cccGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCAttttt                                                                    >  1:286764/1‑140 (MQ=255)

                                                              ccGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTc                                                                   >  2:656663/1‑140 (MQ=255)

                                                               cagcgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCa                                                                  <  2:790243/138‑1 (MQ=255)

                                                               cGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCa                                                                  <  2:493243/140‑1 (MQ=255)

                                                                  cgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattcca                                                                         >  2:1153115/1‑130 (MQ=255)

                                                                  cgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattcca                                                                         <  1:1153115/130‑1 (MQ=255)

                                                                  cgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                               >  2:28559/1‑140 (MQ=255)

                                                                  cgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                               >  1:28696/1‑140 (MQ=255)

                                                                   gcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataat                                                              >  1:208384/1‑140 (MQ=255)

                                                                     ttAAACTCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAatat                                                            >  2:57213/1‑140 (MQ=255)

                                                                     ttAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAatat                                                            >  2:251142/1‑140 (MQ=255)

                                                                     ttAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAatat                                                            >  2:259979/1‑140 (MQ=255)

                                                                       aaaCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTc                                                          >  2:822437/1‑140 (MQ=255)

                                                                         aCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAt                                                        <  2:551078/140‑1 (MQ=255)

                                                                          cccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAtt                                                       <  1:555467/140‑1 (MQ=255)

                                                                             tGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGt                                                    <  1:259979/140‑1 (MQ=255)

                                                                              ggCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGtt                                                   >  1:229584/1‑140 (MQ=255)

                                                                               gCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGtta                                                  <  2:526615/140‑1 (MQ=255)

                                                                               gCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGtta                                                  <  2:561146/140‑1 (MQ=255)

                                                                               gCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGtta                                                  <  1:1075148/140‑1 (MQ=255)

                                                                               gCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGtta                                                  <  2:286764/140‑1 (MQ=255)

                                                                                    ccATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTattatt                                             <  2:389310/140‑1 (MQ=255)

                                                                                     cATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                            <  1:927040/140‑1 (MQ=255)

                                                                                     cATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                            >  2:854962/1‑140 (MQ=255)

                                                                                                 gATTACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACt                                <  2:65434/140‑1 (MQ=255)

                                                                                                    tACGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtct                             <  1:822437/140‑1 (MQ=255)

                                                                                                     aCGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctc                            <  2:867152/140‑1 (MQ=255)

                                                                                                     aCGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctc                            >  1:880540/1‑140 (MQ=255)

                                                                                                      cGCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctct                           <  2:853581/140‑1 (MQ=255)

                                                                                                       gCTGATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctctc                          >  2:96860/1‑140 (MQ=255)

                                                                                                          gATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTTAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTc                       <  1:11461/140‑1 (MQ=255)

                                                                                                          gATCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTc                       <  2:571582/140‑1 (MQ=255)

                                                                                                           aTCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                      <  2:203121/140‑1 (MQ=255)

                                                                                                           aTCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                      <  2:143523/140‑1 (MQ=255)

                                                                                                           aTCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                      <  1:251142/140‑1 (MQ=255)

                                                                                                           aTCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                      <  1:57213/140‑1 (MQ=255)

                                                                                                            tCGTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCat                     >  1:163678/1‑140 (MQ=255)

                                                                                                              gTGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCatat                   <  2:28696/140‑1 (MQ=255)

                                                                                                               tGGGTATCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATc                  <  1:744489/140‑1 (MQ=255)

                                                                                                                     tCCTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATGTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGta            >  2:1023632/1‑140 (MQ=255)

                                                                                                                       cTGTGCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtatt          <  1:853331/140‑1 (MQ=255)

                                                                                                                          tgCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACTATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                      <  2:1056969/125‑1 (MQ=255)

                                                                                                                          tgCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                      >  1:1056969/1‑125 (MQ=255)

                                                                                                                          tgCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa               >  1:1019741/1‑132 (MQ=255)

                                                                                                                          tgCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa               <  2:1019741/132‑1 (MQ=255)

                                                                                                                          tgCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATc       >  1:18832/1‑140 (MQ=255)

                                                                                                                          tgCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATc       >  1:917756/1‑140 (MQ=255)

                                                                                                                           gCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCa      >  1:222885/1‑140 (MQ=255)

                                                                                                                           gCGTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCa      <  1:1120831/140‑1 (MQ=255)

                                                                                                                             gTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagt    <  1:338314/140‑1 (MQ=255)

                                                                                                                             gTATCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagt    <  2:208384/140‑1 (MQ=255)

                                                                                                                               aTCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtta  >  1:43049/1‑140 (MQ=255)

                                                                                                                               aTCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtta  >  1:342389/1‑140 (MQ=255)

                                                                                                                               aTCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtta  >  1:798046/1‑140 (MQ=255)

                                                                                                                               aTCGGCAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtta  >  2:1162461/1‑140 (MQ=255)

TCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTA  >  NC_000913/2986928‑2987194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A