Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	150	55.2	2087638	89.3%	1864260	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,056,402	A→G	E54E (GAA→GAG)	fau →	putative 5‑formyltetrahydrofolate cyclo‑ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,056,402	0	A	G	100.0%	145.2 / NA	41	E54E (GAA→GAG)	fau	putative 5‑formyltetrahydrofolate cyclo‑ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/23); total (18/23)

CGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGG  >  NC_000913/3056272‑3056538

cGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTgg                                                                                                                                 >  1:777102/1‑140 (MQ=255)

          gcagGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTa                                                                                                                       <  2:146641/140‑1 (MQ=255)

          gcagGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTa                                                                                                                       <  1:309905/140‑1 (MQ=255)

           cagGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTTTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTaa                                                                                                                      <  2:721940/140‑1 (MQ=255)

            agGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAg                                                                                                                     <  2:9635/140‑1 (MQ=255)

              gAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAgcg                                                                                                                   <  1:221171/140‑1 (MQ=255)

              gAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAgcg                                                                                                                   <  2:777102/140‑1 (MQ=255)

                         acaaGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTc                                                                                                        >  1:814009/1‑140 (MQ=255)

                            aGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAg                                                                                                     <  1:330688/140‑1 (MQ=255)

                                 cTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTg                                                                                                <  2:598299/140‑1 (MQ=255)

                                   aCCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCa                                                                                              <  2:619686/140‑1 (MQ=255)

                                   aCCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCa                                                                                              <  2:860832/140‑1 (MQ=255)

                                              gaCTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTg                                                                                   >  1:336146/1‑140 (MQ=255)

                                                   aTCCCCCGGTGGTGTTGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCa                                                                                              <  2:807610/124‑1 (MQ=255)

                                                   aTCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCa                                                                                              >  1:807610/1‑124 (MQ=255)

                                                   aTCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGc                                                                                  >  1:180658/1‑136 (MQ=255)

                                                   aTCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGc                                                                                  <  2:180658/136‑1 (MQ=255)

                                                   aTCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGt                                                                              >  2:172502/1‑140 (MQ=255)

                                                        ccGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAAttt                                                                         >  2:116996/1‑140 (MQ=255)

                                                             ggtgATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGt                                                                    <  2:814009/140‑1 (MQ=255)

                                                               tgATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTc                                                                  >  1:940673/1‑140 (MQ=255)

                                                                        aTACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTAc                                                         >  2:1026007/1‑140 (MQ=255)

                                                                              tCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCg                                                   >  2:62622/1‑140 (MQ=255)

                                                                                 cTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCaa                                                >  2:472913/1‑140 (MQ=255)

                                                                                    tATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGc                                             >  1:920245/1‑140 (MQ=255)

                                                                                        ccTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAAc                                         <  1:116996/140‑1 (MQ=255)

                                                                                        ccTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAAc                                         >  2:807892/1‑140 (MQ=255)

                                                                                        ccTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAAc                                         <  1:472913/140‑1 (MQ=255)

                                                                                            tctTTTGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGt                                     <  2:148073/140‑1 (MQ=255)

                                                                                              ttttGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGaa                                          <  2:358788/133‑1 (MQ=255)

                                                                                              ttttGATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGaa                                          >  1:358788/1‑133 (MQ=255)

                                                                                                  gATGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGaa                               <  1:1026007/140‑1 (MQ=255)

                                                                                                   aTGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAAc                              >  2:363184/1‑140 (MQ=255)

                                                                                                   aTGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAAc                              >  2:4292/1‑140 (MQ=255)

                                                                                                   aTGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAAc                              <  2:336146/140‑1 (MQ=255)

                                                                                                   aTGGCGAACTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAAc                              >  1:950579/1‑140 (MQ=255)

                                                                                                          aCTCGACACCCAGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGa                       <  2:367438/140‑1 (MQ=255)

                                                                                                                     aGCCACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAg            >  2:363663/1‑140 (MQ=255)

                                                                                                                       ccACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGcc          <  2:310526/140‑1 (MQ=255)

                                                                                                                       ccACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGcc          <  1:4292/140‑1 (MQ=255)

                                                                                                                       ccACTCATAGAGCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGcc          <  1:170838/140‑1 (MQ=255)

                                                                                                                               agagCAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTgg  <  1:888717/140‑1 (MQ=255)

CGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGG  >  NC_000913/3056272‑3056538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A