Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,864	C→A	Q9K (CAA→AAA)	ubiK →	ubiquinone biosynthesis accessory factor UbiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,864	0	C	A	100.0%	118.2 / NA	34	Q9K (CAA→AAA)	ubiK	ubiquinone biosynthesis accessory factor UbiK
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/8); total (26/8)

AGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCG  >  NC_000913/3184749‑3184996

agcaACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAAt                                                                                                              >  1:433272/1‑140 (MQ=255)

      gCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGc                                                                                                        <  2:380688/140‑1 (MQ=255)

                 caTAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATc                                                                                             >  2:517050/1‑140 (MQ=255)

                    aaTTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAgg                                                                                          <  1:106057/140‑1 (MQ=255)

                         tGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGtt                                                                                     <  1:319502/140‑1 (MQ=255)

                           gTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCg                                                                                   >  2:37402/1‑140 (MQ=255)

                                tGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGaa                                                                              >  2:95765/1‑140 (MQ=255)

                                tGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCT‑AAGGAATCAGGGAGTTCGGGGAAg                                                                             >  1:471233/1‑140 (MQ=255)

                                      aCCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGAtgtg                                                                        <  2:433272/140‑1 (MQ=255)

                                                  cTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATc                                                            >  2:406863/1‑140 (MQ=255)

                                                  cTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATc                                                            >  2:72734/1‑140 (MQ=255)

                                                           cACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAAcc                                                   >  2:588309/1‑140 (MQ=255)

                                                           cACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAAcc                                                   >  2:346682/1‑140 (MQ=255)

                                                             cTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCt                                                 >  1:273305/1‑140 (MQ=255)

                                                               cAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGATCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTAc                                               >  2:275469/1‑140 (MQ=255)

                                                                aaCACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACa                                              >  1:53083/1‑140 (MQ=255)

                                                                aaCACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACa                                              >  1:368099/1‑140 (MQ=255)

                                                                aaCACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACa                                              >  1:59032/1‑140 (MQ=255)

                                                                aaCACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACa                                              >  2:432101/1‑140 (MQ=255)

                                                                     cTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAgcgc                                         >  2:523432/1‑140 (MQ=255)

                                                                               aGGGAAACTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAcgcg                               >  1:572652/1‑140 (MQ=255)

                                                                                 ggAAACTACAATGCTTGACTCGAAAAAAATTGAGAAAATCGCTCGCCAGGTAAACGAATCAATGCCTAAAGGAATCAGGCAGTTAGGGGAAGATGCGGAGAAAAAAATCCGCCAAACCCAACAAGAGCAGCTGACtcgct                             >  2:497040/1‑139 (MQ=255)

                                                                                      cTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGTTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                        >  2:218538/1‑140 (MQ=255)

                                                                                      cTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                        >  1:69524/1‑140 (MQ=255)

                                                                                      cTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                        >  2:279932/1‑140 (MQ=255)

                                                                                      cTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                        >  1:305315/1‑140 (MQ=255)

                                                                                      cTACAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                        >  1:191203/1‑140 (MQ=255)

                                                                                         cAATGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCtt                     >  2:87292/1‑140 (MQ=255)

                                                                                           aTGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGt                   <  2:99514/140‑1 (MQ=255)

                                                                                           aTGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGt                   <  2:540191/140‑1 (MQ=255)

                                                                                           aTGATTGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGt                   <  1:87292/140‑1 (MQ=255)

                                                                                               ttGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGc               >  2:93404/1‑140 (MQ=255)

                                                                                                tGACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGcc              <  1:432101/140‑1 (MQ=255)

                                                                                                 gACCCGAAAAAAATTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGAAATCAGGGTGTTCGGGGAAGATGTGGGGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAATcct             >  2:2763/1‑139 (MQ=255)

                                                                                                        aaaaaaTTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAagag      >  1:144810/1‑140 (MQ=255)

                                                                                                            aaTTGAGAAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCg  <  1:37402/140‑1 (MQ=255)

AGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCG  >  NC_000913/3184749‑3184996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A