Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,052,337	C→A	P98Q (CCG→CAG)	hemN →	coproporphyrinogen III dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,052,337	0	C	A	100.0%	138.0 / NA	40	P98Q (CCG→CAG)	hemN	coproporphyrinogen III dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (27/13); total (27/13)

CGTACATATCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACCGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACTTCCAGTTCAATGCCGATGCGGAGATTT  >  NC_000913/4052212‑4052471

cGTACATATCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGc                                                                                                                          >  2:228665/1‑140 (MQ=255)

  tACATATCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGcgt                                                                                                                        >  2:515430/1‑140 (MQ=255)

  tACATATCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGcgt                                                                                                                        >  1:93237/1‑140 (MQ=255)

      tatCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGcgtcacg                                                                                                                    >  1:223372/1‑140 (MQ=255)

      tatCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGcgtcacg                                                                                                                    >  1:125279/1‑140 (MQ=255)

        tCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGcgtcacgtc                                                                                                                  >  2:269598/1‑140 (MQ=255)

        tCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGcgtcacgtc                                                                                                                  >  1:92834/1‑140 (MQ=255)

         cccGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGcgtcacgtca                                                                                                                 <  2:422285/140‑1 (MQ=255)

         cccGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGcgtcacgtca                                                                                                                 <  1:530336/140‑1 (MQ=255)

             ttCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCa                                                                                                             >  2:446670/1‑140 (MQ=255)

                     tAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACt                                                                                                     >  1:294904/1‑140 (MQ=255)

                         cTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTgggg                                                                                                 <  1:195196/140‑1 (MQ=255)

                            tGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGggcgg                                                                                              >  1:400974/1‑140 (MQ=255)

                                   tCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACg                                                                                       >  1:373908/1‑140 (MQ=255)

                                   tCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACg                                                                                       >  1:75954/1‑140 (MQ=255)

                                                        ttACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAgcg                                                                  >  2:46545/1‑140 (MQ=255)

                                                        ttACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAgcg                                                                  >  1:443291/1‑140 (MQ=255)

                                                                cagcagCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAg                                                          <  1:151977/140‑1 (MQ=255)

                                                                cagcagCACAAGGCAGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATCGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAg                                                          <  1:515430/140‑1 (MQ=255)

                                                                  gcagcaCAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAgcc                                                        >  2:404299/1‑140 (MQ=255)

                                                                   cagcaCAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAgccg                                                       <  2:400974/140‑1 (MQ=255)

                                                                                aTCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAgctg                                          >  2:484150/1‑140 (MQ=255)

                                                                                aTCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAgctg                                          >  2:273864/1‑140 (MQ=255)

                                                                                 tCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAgctgc                                         <  2:431654/140‑1 (MQ=255)

                                                                                 tCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAgctgc                                         <  2:93237/140‑1 (MQ=255)

                                                                                 tCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAgctgc                                         <  1:273864/140‑1 (MQ=255)

                                                                                         tGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGaa                                 <  2:294904/140‑1 (MQ=255)

                                                                                          ggACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGaaa                                >  2:260012/1‑140 (MQ=255)

                                                                                           gACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGaaaa                               >  2:33024/1‑140 (MQ=255)

                                                                                           gACGCGCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGAACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGaaaa                               >  2:119030/1‑140 (MQ=255)

                                                                                             cgcgCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACt                             >  2:356819/1‑140 (MQ=255)

                                                                                             cgcgCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACt                             >  2:211634/1‑140 (MQ=255)

                                                                                             cgcgCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACt                             >  1:71847/1‑140 (MQ=255)

                                                                                             cgcgCTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACt                             >  1:228954/1‑140 (MQ=255)

                                                                                               cgcTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACTTc                           >  1:576306/1‑140 (MQ=255)

                                                                                               cgcTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACTTc                           >  1:523174/1‑140 (MQ=255)

                                                                                               agcTGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACTTc                           <  2:353881/139‑1 (MQ=255)

                                                                                                  tGGAGCAAGAAATCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACTTCCAg                        <  1:228665/140‑1 (MQ=255)

                                                                                                              tCGTCCATCGTGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACTTCCAGTTCAATGCCGAt            >  2:458635/1‑140 (MQ=255)

                                                                                                                        tGCACAGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACTTCCAGTTCAATGCCGATGCGGAGAttt  <  1:85371/140‑1 (MQ=255)

CGTACATATCCCGTTCTGCCATAAGCTTTGTTACTTCTGCGGTTGCAATAAGATTGTTACTCGCCAGCAGCACAAGGCCGATCAGTATCTGGACGCGCTGGAGCAAGAAATCGTCCATCGTGCACCGCTGTTCGCCGGGCGTCACGTCAGCCAATTGCACTGGGGCGGCGGAACGCCGACGTATCTGAATAAAGCGCAAATCAGCCGCCTGATGAAGCTGCTGCGCGAAAACTTCCAGTTCAATGCCGATGCGGAGATTT  >  NC_000913/4052212‑4052471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A