Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,353,995	C→A	E241D (GAG→GAT)	lysU ←	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,353,995	0	C	A	100.0%	109.7 / NA	32	E241D (GAG→GAT)	lysU	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/18); total (14/18)

TCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAA  >  NC_000913/4353861‑4354129

tCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGACCCgg                                                                                                                                   >  2:363325/1‑140 (MQ=255)

  cGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGgcg                                                                                                                                 <  1:15916/140‑1 (MQ=255)

  cGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGgcg                                                                                                                                 >  2:15916/1‑140 (MQ=255)

     aTACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGa                                                                                                                              >  1:574910/1‑140 (MQ=255)

     aTACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGa                                                                                                                              <  2:255027/140‑1 (MQ=255)

       aCGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATa                                                                                                                            <  1:70348/140‑1 (MQ=255)

         gCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACg                                                                                                                          >  1:101474/1‑140 (MQ=255)

               tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGACCCGGCGCGATACGCAGATa                                                                                                                    <  1:363325/140‑1 (MQ=255)

                 gagTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACa                                                                                                                  <  1:299147/140‑1 (MQ=255)

                 gagTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACa                                                                                                                  <  1:422990/140‑1 (MQ=255)

                      ccATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCt                                                                                                             <  1:370733/140‑1 (MQ=255)

                      ccATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCt                                                                                                             <  1:403418/140‑1 (MQ=255)

                             tgtgAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCa                                                                                                      <  2:12635/140‑1 (MQ=255)

                             tgtgAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCa                                                                                                      <  2:574910/140‑1 (MQ=255)

                             tgggAACTTAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCa                                                                                                      <  1:333410/137‑1 (MQ=255)

                                      aGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTat                                                                                             >  1:56055/1‑140 (MQ=255)

                                             tGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGt                                                                                      >  1:395829/1‑140 (MQ=255)

                                                cGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaat                                                                                   >  2:242140/1‑140 (MQ=255)

                                                cGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaat                                                                                   >  1:62201/1‑140 (MQ=255)

                                                                       gAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCAc                                                            <  2:112880/140‑1 (MQ=255)

                                                                          gTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTg                                                         >  2:276727/1‑140 (MQ=255)

                                                                                           aTACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCatcatc                                        <  1:418029/140‑1 (MQ=255)

                                                                                           aTACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCatcatc                                        >  2:425073/1‑140 (MQ=255)

                                                                                                   tCAAAACCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGtttt                                >  2:210088/1‑139 (MQ=255)

                                                                                                      aaaCCGCCTACGACCAGACGTTTCAGATGCAGATCCGGCGCGATACGCAGATACGTATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTAc                             <  2:25238/140‑1 (MQ=255)

                                                                                                      aaaCCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTAc                             <  2:123508/140‑1 (MQ=255)

                                                                                                      aaaCCGCCTACAACCAGACGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTAc                             <  1:171969/140‑1 (MQ=255)

                                                                                                                       aCGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgcg            <  2:12197/140‑1 (MQ=255)

                                                                                                                       aCGTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgcg            <  1:527817/140‑1 (MQ=255)

                                                                                                                         gTTTCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgcgcg          <  2:363608/140‑1 (MQ=255)

                                                                                                                           ttCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGAc        >  1:419661/1‑140 (MQ=255)

                                                                                                                           ttCAGATACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGAc        >  1:131808/1‑140 (MQ=255)

                                                                                                                                 tACAGATCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGaa  >  2:273638/1‑140 (MQ=255)

TCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAA  >  NC_000913/4353861‑4354129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A