Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F48 I1 R1	52	61.9	2103308	98.8%	2078068	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,193	G→T	A42E (GCG→GAG)	ubiI ←	2‑octaprenylphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,193	0	G	T	97.0%	221.2 / 0.1	67	A42E (GCG→GAG)	ubiI	2‑octaprenylphenol hydroxylase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (35/30); total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

GTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAT  >  NC_000913/3052063‑3052324

gTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCagag                                                                                                                            <  2:830916/140‑1 (MQ=255)

  cccACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGt                                                                                                                          >  2:45329/1‑140 (MQ=255)

     acacTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTcc                                                                                                                       >  1:916776/1‑140 (MQ=255)

     acacTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTcc                                                                                                                       >  2:132600/1‑140 (MQ=255)

     acacTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTcc                                                                                                                       >  1:612676/1‑140 (MQ=255)

     acacTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTcc                                                                                                                       >  1:27234/1‑140 (MQ=255)

           ccATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTAcg                                                                                                                 <  1:497719/140‑1 (MQ=255)

             aTACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGTTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTAcgcg                                                                                                               <  2:537523/140‑1 (MQ=255)

              tACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACgcgc                                                                                                              >  1:796769/1‑140 (MQ=255)

                 cGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCgctgc                                                                                                           <  1:132600/140‑1 (MQ=255)

                 cGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCgctgc                                                                                                           >  1:337863/1‑140 (MQ=255)

                 cGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCgctgc                                                                                                           <  1:405349/140‑1 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTc                                                                                                                        >  2:442730/1‑125 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTc                                                                                                                        <  1:442730/125‑1 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  2:27485/1‑140 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  1:580950/1‑140 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         <  1:76712/140‑1 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  1:8234/1‑140 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  1:466935/1‑140 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         <  1:45329/140‑1 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  1:381753/1‑140 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  2:950289/1‑140 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  1:218241/1‑140 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  2:956905/1‑140 (MQ=255)

                   tGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTc                                                                                                         >  1:1003891/1‑140 (MQ=255)

                     aTAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCa                                                                                                       >  2:162958/1‑140 (MQ=255)

                     aTAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCACCa                                                                                                       >  1:655558/1‑140 (MQ=255)

                     aTAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCACCAGAGGTTCCTGTACGCGCTGCTCCa                                                                                                       >  1:504139/1‑140 (MQ=255)

                     aTAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCa                                                                                                       >  2:21856/1‑140 (MQ=255)

                           gCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACgg                                                                                                 >  2:659424/1‑140 (MQ=255)

                            cTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGc                                                                                                >  1:818519/1‑140 (MQ=255)

                            cTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCACCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGc                                                                                                <  2:504139/140‑1 (MQ=255)

                             tGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCa                                                                                               >  2:962418/1‑140 (MQ=255)

                             tGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCa                                                                                               >  1:105907/1‑140 (MQ=255)

                              ggCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCaa                                                                                              >  1:1013759/1‑140 (MQ=255)

                               gCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAAc                                                                                             >  1:674547/1‑140 (MQ=255)

                               gCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAAc                                                                                             <  1:21856/140‑1 (MQ=255)

                                    aCGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTa                                                                                        >  2:629846/1‑140 (MQ=255)

                                    aCGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTa                                                                                        <  1:630129/140‑1 (MQ=255)

                                      gagagagaATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTaac                                                                                      >  1:397797/1‑139 (MQ=255)

                                      gagagagaATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAg                                                                                      <  2:470673/140‑1 (MQ=255)

                                         agagaATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCg                                                                                   <  1:956905/140‑1 (MQ=255)

                                           agaATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCt                                                                                 >  2:595270/1‑140 (MQ=255)

                                           agaATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGCTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCt                                                                                 <  2:818519/140‑1 (MQ=255)

                                            aaaTGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTc                                                                                <  2:8234/139‑1 (MQ=255)

                                              aTGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTccc                                                                              <  2:218241/140‑1 (MQ=255)

                                              aTGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTccc                                                                              <  1:629846/140‑1 (MQ=255)

                                              aTGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTccc                                                                              <  2:466935/140‑1 (MQ=255)

                                                 tCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTg                                                                           >  1:203280/1‑140 (MQ=255)

                                                   cTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTa                                                                         <  2:381753/140‑1 (MQ=255)

                                                    tGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTaa                                                                        >  2:310983/1‑140 (MQ=255)

                                                             ccAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGc                                                               <  2:674547/140‑1 (MQ=255)

                                                              cAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCa                                                              <  1:595270/140‑1 (MQ=255)

                                                                  aCGGGTGAGTAATTTTTCGCTGGCGGCATTGATGCCCAAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGGGGTTCCTGTACGCAATGCCCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCAAAGAAAACCg                                                          <  2:612676/140‑1 (MQ=255)

                                                                   ggggTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAACCGCGCAGTTGTGGTGGTGCATTCTCCCCCAGAGGTTCCTGTACGCGCTGCTCCAGTCCGGCACCGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGc                                                         <  2:816485/139‑1 (MQ=255)

                                                                    gggTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGGGCATTCTCCGCCAGAGGTCCCTGTCCGCGCTGCTCCAGTCCGGCACCGCGTAAGCCGCTCCCCTGAAAGCCACAGGCAACCGcc                                                        <  2:765721/140‑1 (MQ=255)

                                                                      gTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCACCGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAg                                                      <  2:27234/140‑1 (MQ=255)

                                                                      gTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGAAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAg                                                      <  1:977611/140‑1 (MQ=255)

                                                                                tttCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATg                                            <  1:297426/140‑1 (MQ=255)

                                                                                                 aTAGCCGAACCGCGCATTTGTGGTGGTGCATTCTCCGCCAGAGTTCCCTGTGCGCGCGGCTCCAGTCGGGCAAGGCGAAGGCCCCCCCCTTGTAAGCCACAGCCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGc                           <  2:565910/140‑1 (MQ=255)

                                                                                                 aTAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGc                           <  1:612813/140‑1 (MQ=255)

                                                                                                 aTAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGc                           <  1:962418/140‑1 (MQ=255)

                                                                                                 aTAGCCGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCCCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGc                           <  2:916776/140‑1 (MQ=255)

                                                                                                 aTAGCCGAAACGCGCAGTTGTGGTGGTGCATCCCCCCCCAGAGGTTCCTGTACGCGCTCCCCCAGTCCGGCAACGCGTAAGCCGCCCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGc                           <  2:864384/140‑1 (MQ=255)

                                                                                                     ccGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCTGTACGGCAACCCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACa                       >  2:696586/1‑140 (MQ=255)

                                                                                                      cGAAACGCGCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACAt                      >  1:364808/1‑140 (MQ=255)

                                                                                                           cgcgCAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGAAACGCGTAAGCCGCCCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAaca                 <  2:1003891/140‑1 (MQ=255)

                                                                                                            gcgcATTTGTGGTGCTGCATCCTCCGCCAGGGGTTCCTGTACGCGCTGATCCAGTGCGCAACCGCGAAAGCCGCTCCCCTATATGCCACAGCCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAacac                <  2:203280/140‑1 (MQ=255)

                                                                                                             cgcAGTTGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACt               <  1:278846/140‑1 (MQ=255)

                                                                                                                  ttGTGGTGGTGCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCCCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCa          <  2:580950/140‑1 (MQ=255)

                                                                                                                       gtggtgCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGtt     >  2:100161/1‑140 (MQ=255)

                                                                                                                         ggtgCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAGGCCGCTCCCCTGTAAGCCACAGGAAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTa   <  2:129748/140‑1 (MQ=255)

                                                                                                                         ggtgCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTa   <  1:27485/140‑1 (MQ=255)

                                                                                                                         ggtgCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTa   <  1:659424/140‑1 (MQ=255)

                                                                                                                          gtgCATTCTCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt  >  1:199347/1‑140 (MQ=255)

GTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAT  >  NC_000913/3052063‑3052324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A