Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F48 I1 R1	52	61.9	2103308	98.8%	2078068	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,399,968	G→T	L239F (TTG→TTT)	miaA →	tRNA dimethylallyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,399,968	0	G	T	100.0%	230.0 / NA	65	L239F (TTG→TTT)	miaA	tRNA dimethylallyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (33/32); total (33/32)

CGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAAC  >  NC_000913/4399832‑4400102

cGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCAt                                                                                                                                     >  2:256634/1‑140 (MQ=255)

 gTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATa                                                                                                                                    >  1:75121/1‑140 (MQ=255)

  tATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATAc                                                                                                                                   >  2:396808/1‑140 (MQ=255)

  tATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATAc                                                                                                                                   >  2:260996/1‑140 (MQ=255)

    tCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACgg                                                                                                                                 >  2:734508/1‑140 (MQ=255)

       ggTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACt                                                                                                                              <  2:482579/140‑1 (MQ=255)

       ggTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACt                                                                                                                              >  2:966971/1‑140 (MQ=255)

       ggTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGC‑TTTTGCCCGAGGAGATTTTCATACGGACtt                                                                                                                             >  1:44911/1‑140 (MQ=255)

         tGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACttt                                                                                                                            >  2:79077/1‑139 (MQ=255)

         tGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTg                                                                                                                            >  2:113893/1‑140 (MQ=255)

         tGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTg                                                                                                                            >  1:411506/1‑140 (MQ=255)

         tGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTg                                                                                                                            >  2:226638/1‑140 (MQ=255)

           cATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGcc                                                                                                                          >  2:668703/1‑140 (MQ=255)

           cATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGcc                                                                                                                          >  2:92856/1‑140 (MQ=255)

            aTCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCt                                                                                                                         >  2:244917/1‑140 (MQ=255)

            aTCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCt                                                                                                                         >  2:909056/1‑140 (MQ=255)

            aTCAGTTCGCCATCGCCCCGGCGACCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCt                                                                                                                         >  2:344369/1‑140 (MQ=255)

               aGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTcc                                                                                                                      >  2:295083/1‑140 (MQ=255)

                    gCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCg                                                                                                                 <  1:14570/140‑1 (MQ=255)

                    gCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCg                                                                                                                 <  1:865842/140‑1 (MQ=255)

                          gCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGt                                                                                                           <  1:396808/140‑1 (MQ=255)

                          gCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGt                                                                                                           <  1:226638/140‑1 (MQ=255)

                          gCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGt                                                                                                           <  1:113893/140‑1 (MQ=255)

                           ccccGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTg                                                                                                          >  1:471560/1‑140 (MQ=255)

                           ccccGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTg                                                                                                          >  1:1008181/1‑140 (MQ=255)

                             ccGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTggg                                                                                                        <  2:471560/140‑1 (MQ=255)

                               ggCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGtt                                                                                                      <  2:1038790/140‑1 (MQ=255)

                                gCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTa                                                                                                     <  1:692433/140‑1 (MQ=255)

                                  gAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATc                                                                                                   <  1:256634/140‑1 (MQ=255)

                                    gCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGc                                                                                                 <  1:260996/140‑1 (MQ=255)

                                    gCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGc                                                                                                 >  2:417039/1‑140 (MQ=255)

                                    gCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTATCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGc                                                                                                 <  1:741524/140‑1 (MQ=255)

                                     ccGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGcc                                                                                                >  1:442608/1‑140 (MQ=255)

                                          aaCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtg                                                                                           <  2:274788/140‑1 (MQ=255)

                                          aaCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtg                                                                                           <  1:824307/140‑1 (MQ=255)

                                          aaCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtg                                                                                           >  1:296881/1‑140 (MQ=255)

                                          aaCTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtg                                                                                           <  2:232459/140‑1 (MQ=255)

                                          aaCTGCTCCAACAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtg                                                                                           <  1:144897/140‑1 (MQ=255)

                                               cTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTc                                                                                      >  2:2565/1‑140 (MQ=255)

                                                tCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCt                                                                                     <  1:286765/140‑1 (MQ=255)

                                                      aaCGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCtt                                                                               <  1:909056/140‑1 (MQ=255)

                                                       aCGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTg                                                                              <  1:295083/140‑1 (MQ=255)

                                                         gCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGaa                                                                            >  1:15045/1‑140 (MQ=255)

                                                             tGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTGCCTTGAAGGCg                                                                        <  1:244917/140‑1 (MQ=255)

                                                             tGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCg                                                                        <  2:442608/140‑1 (MQ=255)

                                                             tGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCg                                                                        <  1:2565/140‑1 (MQ=255)

                                                               agcagcGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGaa                                                                      <  2:75121/140‑1 (MQ=255)

                                                               agcagcGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGaa                                                                      <  2:296881/140‑1 (MQ=255)

                                                                       tttCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATa                                                              <  1:734508/140‑1 (MQ=255)

                                                                              aGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGaa                                                       <  1:547071/140‑1 (MQ=255)

                                                                              aGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGaa                                                       >  1:665628/1‑140 (MQ=255)

                                                                               gATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGaaa                                                      <  1:344369/140‑1 (MQ=255)

                                                                                   ttGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGt                                                  >  2:213656/1‑140 (MQ=255)

                                                                                     ggCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGttt                                                >  1:684902/1‑140 (MQ=255)

                                                                                         tCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCg                                            <  1:966971/140‑1 (MQ=255)

                                                                                          cAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGa                                           >  1:953603/1‑140 (MQ=255)

                                                                                           aGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAg                                          >  1:978613/1‑140 (MQ=255)

                                                                                               tttGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGgtgt                                      <  2:411506/140‑1 (MQ=255)

                                                                                                  gAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTg                                   <  2:953603/140‑1 (MQ=255)

                                                                                                          aGTCCGGGCGCTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACga                           <  2:378247/140‑1 (MQ=255)

                                                                                                                 gcgcTTTTTGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGtt                    >  1:389558/1‑140 (MQ=255)

                                                                                                                      ttttGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGa               >  1:506955/1‑140 (MQ=255)

                                                                                                                         tGCCCGAGGAGATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGc            <  2:978613/140‑1 (MQ=255)

                                                                                                                               aggagATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGa      >  1:632539/1‑140 (MQ=255)

                                                                                                                               aggagATTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGa      >  1:582751/1‑140 (MQ=255)

                                                                                                                                   gaTTTTCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAAc  <  2:1008181/140‑1 (MQ=255)

CGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAAC  >  NC_000913/4399832‑4400102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A