Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,139,723 C→A S81I (AGC→ATC)  hybG ← hydrogenase 2 accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,139,7230CA100.0% 478.0 / NA 139S81I (AGC→ATC) hybGhydrogenase 2 accessory protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (69/70);  total (69/70)

Only 100 of 140 total aligned reads displayed.

AGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAAT  >  NC_000913/3139586‑3139859
                                                                                                                                         |                                                                                                                                        
aGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCGCAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATg                                                                                                                                        >  2:88947/1‑140 (MQ=255)
aGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATg                                                                                                                                        >  1:597969/1‑140 (MQ=255)
aGGGGGAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATg                                                                                                                                        >  1:2100537/1‑140 (MQ=255)
 ggggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATgg                                                                                                                                       >  2:1967366/1‑140 (MQ=255)
 ggggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATgg                                                                                                                                       >  1:1675073/1‑140 (MQ=255)
 ggggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATgg                                                                                                                                       <  2:275383/140‑1 (MQ=255)
   gttAAGGTTTGGTGTACATCGCAGCAAGCAATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTa                                                                                                                                     <  2:411121/138‑1 (MQ=25)
   ggTAGGGGTTGGTGTTCGGCGCAGCAAGCCAGCCAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTa                                                                                                                                     <  2:4457/140‑1 (MQ=37)
     tAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAAt                                                                                                                                   >  1:2077630/1‑140 (MQ=255)
            tGGTGTTCGTTGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTaa                                                                                                                            >  2:1635461/1‑140 (MQ=255)
            tGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTaa                                                                                                                            >  2:2055862/1‑140 (MQ=255)
            tGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTaa                                                                                                                            >  2:1605472/1‑140 (MQ=255)
             ggTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAAt                                                                                                                           >  1:487715/1‑140 (MQ=255)
             ggTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTAGTAAt                                                                                                                           >  2:1319133/1‑140 (MQ=255)
              gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATc                                                                                                                          >  1:1514896/1‑140 (MQ=255)
                  tcgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCAtt                                                                                                                      <  1:1610919/140‑1 (MQ=255)
                   cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTa                                                                                                                                     >  2:160692/1‑124 (MQ=255)
                   cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTa                                                                                                                                     <  1:160692/124‑1 (MQ=255)
                   cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCAttt                                                                                                                     >  2:1239415/1‑140 (MQ=255)
                   cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCAttt                                                                                                                     >  2:52844/1‑140 (MQ=255)
                   cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCAttt                                                                                                                     >  2:1827590/1‑140 (MQ=255)
                    gtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTg                                                                                                                    >  2:1198685/1‑140 (MQ=255)
                     tcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTgg                                                                                                                   <  2:862150/140‑1 (MQ=255)
                       gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGgcg                                                                                                                 <  1:556895/140‑1 (MQ=255)
                       gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGgcg                                                                                                                 <  2:232107/140‑1 (MQ=255)
                       gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGgcg                                                                                                                 <  1:1262483/140‑1 (MQ=255)
                        cagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGgcgc                                                                                                                >  1:1095867/1‑140 (MQ=255)
                         agcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCa                                                                                                               <  2:2100537/140‑1 (MQ=255)
                         agcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCa                                                                                                               <  2:259746/140‑1 (MQ=255)
                         agcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCa                                                                                                               <  1:1605472/140‑1 (MQ=255)
                          gcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAg                                                                                                              <  2:200358/140‑1 (MQ=255)
                             aGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGc                                                                                                           >  1:437018/1‑140 (MQ=255)
                               ccATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGt                                                                                                         <  1:1792727/140‑1 (MQ=255)
                                cATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTc                                                                                                        <  1:1319133/140‑1 (MQ=255)
                                  tCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTa                                                                                                      >  1:915307/1‑140 (MQ=255)
                                         cGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggc                                                                                               >  2:707381/1‑140 (MQ=255)
                                          ggATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggct                                                                                              <  2:916738/140‑1 (MQ=255)
                                           gATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctt                                                                                             <  1:1827590/140‑1 (MQ=255)
                                            aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggcttt                                                                                            >  2:1876318/1‑140 (MQ=255)
                                            aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGAGCGTCTAATGtggcttt                                                                                            >  1:712071/1‑140 (MQ=255)
                                                 gCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggctt                                                                                       <  2:1514896/140‑1 (MQ=255)
                                                 gCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggctt                                                                                       >  1:1196118/1‑140 (MQ=255)
                                                    ttCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCAt                                                                                    >  2:1118957/1‑140 (MQ=255)
                                                     tCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATc                                                                                   <  2:1739697/140‑1 (MQ=255)
                                                     tCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATc                                                                                   <  1:1093093/140‑1 (MQ=255)
                                                     tCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCACCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATc                                                                                   <  2:1828594/140‑1 (MQ=255)
                                                      cACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCt                                                                                  >  1:2031725/1‑140 (MQ=255)
                                                      cACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCt                                                                                  >  1:363207/1‑140 (MQ=255)
                                                       aCGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCtt                                                                                 <  1:885357/140‑1 (MQ=255)
                                                          ccgcATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtcgt                                                                              <  1:388937/140‑1 (MQ=255)
                                                               tCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCgatg                                                                         <  2:1287980/140‑1 (MQ=255)
                                                                    cAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCt                                                                    <  1:245994/140‑1 (MQ=255)
                                                                       tCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCAt                                                                 <  2:487715/140‑1 (MQ=255)
                                                                         gTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATgg                                                               <  1:1126512/140‑1 (MQ=255)
                                                                                 tGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCg                                                       <  1:88947/140‑1 (MQ=255)
                                                                                 tGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCg                                                       >  1:219018/1‑140 (MQ=255)
                                                                                 tGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCg                                                       <  2:896061/140‑1 (MQ=255)
                                                                                    cTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACg                                                    <  1:898320/140‑1 (MQ=255)
                                                                                         gCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGTAAATCCGACGTGTAc                                               <  2:1196118/140‑1 (MQ=255)
                                                                                         gCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATc                                                         >  1:2051513/1‑130 (MQ=255)
                                                                                           gACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCa                                             >  1:2145904/1‑140 (MQ=255)
                                                                                             cGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGc                                           <  1:1641011/140‑1 (MQ=255)
                                                                                              gCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCa                                          <  1:1850123/140‑1 (MQ=255)
                                                                                                   cgcgTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCAc                                     <  2:444044/140‑1 (MQ=255)
                                                                                                   cgcgTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCAc                                     <  2:1623468/140‑1 (MQ=255)
                                                                                                   cgcgTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCAc                                     <  2:1406936/140‑1 (MQ=255)
                                                                                                   cgcgTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCAc                                     <  2:2148438/140‑1 (MQ=255)
                                                                                                      gTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTgg                                  <  2:712071/140‑1 (MQ=255)
                                                                                                      gTCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTgg                                  <  2:219018/140‑1 (MQ=255)
                                                                                                      atCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTgg                                  <  2:597969/139‑1 (MQ=255)
                                                                                                       tCTTATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGc                                 >  2:1485225/1‑140 (MQ=255)
                                                                                                         ttATCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGccc                               <  1:1876318/140‑1 (MQ=255)
                                                                                                            tCAGGCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGt                            >  2:146509/1‑140 (MQ=255)
                                                                                                             agggCCTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTa                           >  1:1913659/3‑140 (MQ=255)
                                                                                                                 ccTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATc                       >  2:443627/1‑140 (MQ=255)
                                                                                                                  cTACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCg                      <  1:443627/140‑1 (MQ=255)
                                                                                                                   tACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCgg                     <  2:249746/140‑1 (MQ=255)
                                                                                                                   tACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCgg                     <  2:915307/140‑1 (MQ=255)
                                                                                                                   tACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCgg                     <  2:1822748/140‑1 (MQ=255)
                                                                                                                   tACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCgg                     <  2:1435993/140‑1 (MQ=255)
                                                                                                                   tACGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCgg                     >  2:1659873/1‑140 (MQ=255)
                                                                                                                    aCGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGc                    >  2:2071437/1‑140 (MQ=255)
                                                                                                                    aCGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGc                    >  1:1919027/1‑140 (MQ=255)
                                                                                                                    aCGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGc                    >  1:1801689/1‑140 (MQ=255)
                                                                                                                    aCGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGc                    >  2:634504/1‑140 (MQ=255)
                                                                                                                    aCGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGc                    >  2:1708867/1‑140 (MQ=255)
                                                                                                                    aCGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGc                    >  1:1262154/1‑140 (MQ=255)
                                                                                                                     cGAAAGCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCa                   >  2:1998756/1‑140 (MQ=255)
                                                                                                                          gCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGtt              >  2:605544/1‑140 (MQ=255)
                                                                                                                          gCTAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGtt              >  1:404154/1‑140 (MQ=255)
                                                                                                                            tAATCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAc            <  2:782429/140‑1 (MQ=255)
                                                                                                                             aaTCATCACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAcc           <  1:146509/140‑1 (MQ=255)
                                                                                                                              atcatcACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCt          >  2:799829/1‑140 (MQ=255)
                                                                                                                              atcatcACGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCt          >  1:1700825/1‑140 (MQ=255)
                                                                                                                                 atcaCGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTca       <  1:634504/140‑1 (MQ=255)
                                                                                                                                   caCGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTcaca     >  1:950464/1‑140 (MQ=255)
                                                                                                                                    aCGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACaa    >  2:191224/1‑140 (MQ=255)
                                                                                                                                    aCGCGATGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACaa    >  1:105943/1‑140 (MQ=255)
                                                                                                                                    aCGCGATGGTAACGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCAAGTAGATCGGCAGGGTTTCCTTCaaaa    >  2:1181089/1‑140 (MQ=255)
                                                                                                                                      gcgaTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAAt  <  1:534572/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAAT  >  NC_000913/3139586‑3139859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A