Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,345,580	A→G	E288E (GAA→GAG)	rpoN →	RNA polymerase, sigma 54 (sigma N) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,345,580	0	A	G	99.4%	631.1 / ‑4.9	164	E288E (GAA→GAG)	rpoN	RNA polymerase, sigma 54 (sigma N) factor
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (84/79); total (85/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 167 total aligned reads displayed.

AAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTG  >  NC_000913/3345444‑3345715

 agaTGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCa                                                                                                                                     <  2:1624767/140‑1 (MQ=255)

  gaTGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCaa                                                                                                                                    >  1:1009389/1‑140 (MQ=255)

   aTGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAAc                                                                                                                                   <  1:1421490/140‑1 (MQ=255)

    tgtgCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACa                                                                                                                                  >  1:673314/1‑140 (MQ=255)

     gtgCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAg                                                                                                                                 <  1:519859/140‑1 (MQ=255)

     gtgCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAg                                                                                                                                 >  1:835856/1‑140 (MQ=255)

      tgCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGt                                                                                                                                >  1:356241/1‑140 (MQ=255)

      tgCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGt                                                                                                                                >  2:1616843/1‑140 (MQ=255)

        cTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGATTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTTGACGGTAGAGCTCAACAGTGa                                                                                                                              >  1:1524331/1‑140 (MQ=255)

        cTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGa                                                                                                                              >  1:688426/1‑140 (MQ=255)

          gAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACa                                                                                                                            >  1:840167/1‑140 (MQ=255)

                agCCGTCATTCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTc                                                                                                                      >  1:1455617/1‑140 (MQ=255)

                agCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTc                                                                                                                      <  2:767303/140‑1 (MQ=255)

                  ccGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCcg                                                                                                                    <  1:855418/140‑1 (MQ=255)

                     tCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGt                                                                                                                 >  2:2024838/1‑140 (MQ=255)

                            gATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCaaa                                                                                                          <  2:638931/140‑1 (MQ=255)

                            gATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCaaa                                                                                                          <  1:496205/140‑1 (MQ=255)

                                 aGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAAc                                                                                                     <  1:1147181/140‑1 (MQ=255)

                                 aGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATGCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAAc                                                                                                     >  1:1738742/1‑140 (MQ=255)

                                  gTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAAcc                                                                                                    >  1:1403383/1‑140 (MQ=255)

                                   tCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATTTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCa                                                                                                   >  2:362047/1‑140 (MQ=255)

                                   tCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCa                                                                                                   >  2:183587/1‑140 (MQ=255)

                                   tCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCa                                                                                                   >  2:1274588/1‑140 (MQ=255)

                                      cTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCa                                                                                                <  1:2024838/140‑1 (MQ=255)

                                      cTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCa                                                                                                >  1:1178296/1‑140 (MQ=255)

                                      cTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCa                                                                                                <  2:1009389/140‑1 (MQ=255)

                                       tCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCAc                                                                                               >  1:257703/1‑140 (MQ=255)

                                       tCGATCCGCGCCCCGGGCAGGCGATCCAGACTGGCGAACCAGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCat                                                                                               >  2:1732119/1‑139 (MQ=255)

                                          aTCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTAc                                                                                            >  2:1252571/1‑140 (MQ=255)

                                           tCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACg                                                                                           >  1:2083934/1‑140 (MQ=255)

                                           tCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACg                                                                                           <  2:1723690/140‑1 (MQ=255)

                                              gcgcCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCAGCGTCTGCAAATCAACCAGCACTACGCCt                                                                                        <  1:932561/140‑1 (MQ=255)

                                                gcCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATTTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCg                                                                                      <  1:362047/140‑1 (MQ=255)

                                                gcCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCg                                                                                      <  1:9190/140‑1 (MQ=255)

                                                gcCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCg                                                                                      <  2:1403383/140‑1 (MQ=255)

                                                gcCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCg                                                                                      <  1:710717/140‑1 (MQ=255)

                                                  cccGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCGGCACTACGCCTCGAt                                                                                    <  2:5636/140‑1 (MQ=255)

                                                  cccGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGAt                                                                                    <  1:863638/140‑1 (MQ=255)

                                                            cGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAAc                                                                          >  2:1694822/1‑140 (MQ=255)

                                                               tCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAAcgcg                                                                       <  1:771771/140‑1 (MQ=255)

                                                                 cAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAAcgcgcg                                                                     <  2:136339/140‑1 (MQ=255)

                                                                   gACTGGCTAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCa                                                                   <  1:2074684/140‑1 (MQ=255)

                                                                       ggCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGa                                                               <  1:1346715/140‑1 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGAtt                                                             >  2:968311/1‑139 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  1:495292/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  1:1421082/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  2:1867064/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  1:1883135/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  2:1188560/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  2:1146016/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  1:1971590/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  2:811522/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  1:370253/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATg                                                             >  1:433227/1‑140 (MQ=255)

                                                                         cGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTACAATAACGCGCGCAACGAtt                                                             >  2:765978/1‑139 (MQ=255)

                                                                             ccTGAGAATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGa                                                         >  2:1788068/1‑140 (MQ=255)

                                                                               tGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGATAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACa                                                       <  2:1282746/140‑1 (MQ=255)

                                                                                 aGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGCAGAGCTCAACAGTGCCAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACACGCGCAACGATGGTGACAGc                                                     >  2:2003135/1‑140 (MQ=255)

                                                                                  gTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGcc                                                    <  1:811522/140‑1 (MQ=255)

                                                                                  gTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGcc                                                    >  2:346808/1‑140 (MQ=255)

                                                                                     tGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGt                                                 <  1:1717660/140‑1 (MQ=255)

                                                                                       tCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGttt                                               >  2:2134009/1‑140 (MQ=255)

                                                                                        cATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTa                                              >  2:2151801/1‑140 (MQ=255)

                                                                                         aTTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTAt                                             >  1:247598/1‑140 (MQ=255)

                                                                                           tCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATcc                                           >  1:1142883/1‑140 (MQ=255)

                                                                                           tCCAGATGTGCTGGTGCGTAAGCATAAC‑GTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCg                                          >  1:457727/1‑140 (MQ=255)

                                                                                              aGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCgca                                        <  2:862508/140‑1 (MQ=255)

                                                                                                aTGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCgcagc                                      <  2:1178296/140‑1 (MQ=255)

                                                                                                 tgtgCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCgcagca                                     <  2:1544808/140‑1 (MQ=255)

                                                                                                 tgtgCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCgcagca                                     <  1:1264309/140‑1 (MQ=255)

                                                                                                     cTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGTTGACAGCCAGTTTATCCGCAGCAATCt                                 >  1:1312583/1‑140 (MQ=255)

                                                                                                     cTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCt                                 >  1:1032454/1‑140 (MQ=255)

                                                                                                     cTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCt                                 >  2:944819/1‑140 (MQ=255)

                                                                                                       ggTGCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGc                               <  2:433227/140‑1 (MQ=255)

                                                                                                          gCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAgg                            <  2:840167/140‑1 (MQ=255)

                                                                                                          gCGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAgg                            <  2:688426/140‑1 (MQ=255)

                                                                                                           cGTAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGa                           >  1:1476068/1‑140 (MQ=255)

                                                                                                             tAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATg                         >  1:1480038/1‑140 (MQ=255)

                                                                                                             tAAGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATg                         >  2:29312/1‑140 (MQ=255)

                                                                                                               aGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGcc                       <  2:495292/140‑1 (MQ=255)

                                                                                                               aGCATAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCGGCAGGATGcc                       <  1:1694822/140‑1 (MQ=255)

                                                                                                               aGCATAAC‑GTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCa                      <  2:457727/140‑1 (MQ=255)

                                                                                                                   tAACGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAAt                   <  2:1061549/140‑1 (MQ=255)

                                                                                                                    aaCGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATg                  >  1:1103946/1‑140 (MQ=255)

                                                                                                                     aCGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATgg                 <  2:1971590/140‑1 (MQ=255)

                                                                                                                     aCGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATgg                 <  1:183587/140‑1 (MQ=255)

                                                                                                                     aCGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATgg                 <  1:218194/140‑1 (MQ=255)

                                                                                                                     aCGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATgg                 <  1:1867064/140‑1 (MQ=255)

                                                                                                                     aCGGTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATgg                 <  1:1213408/140‑1 (MQ=255)

                                                                                                                       ggTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGtt               <  1:582971/140‑1 (MQ=255)

                                                                                                                       ggTCACTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGtt               <  2:370253/140‑1 (MQ=255)

                                                                                                                           aCTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCaa                     >  1:1608032/1‑130 (MQ=255)

                                                                                                                           aCTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATc           >  2:437499/1‑140 (MQ=255)

                                                                                                                           aCTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATc           <  2:673314/140‑1 (MQ=255)

                                                                                                                           aCTGGACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCCGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATc           <  2:1455617/140‑1 (MQ=255)

                                                                                                                               gACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAaga       <  1:437499/140‑1 (MQ=255)

                                                                                                                               gACGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAaga       <  2:2083934/140‑1 (MQ=255)

                                                                                                                               aaCGGTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAaga       <  2:247598/139‑1 (MQ=255)

                                                                                                                                   gTAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCt   >  1:2108186/1‑140 (MQ=255)

                                                                                                                                    tAGAGCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTg  >  1:1225884/1‑140 (MQ=255)

AAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGGCGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTG  >  NC_000913/3345444‑3345715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A