Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,659,143	C→A	intergenic (+61/‑89)	gadF → / → mdtE	small regulatory RNA GadF/multidrug efflux pump membrane fusion protein MdtE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,659,143	0	C	A	100.0%	500.0 / NA	146	intergenic (+61/‑89)	gadF/mdtE	small regulatory RNA GadF/multidrug efflux pump membrane fusion protein MdtE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (69/77); total (69/77)

Only 100 of 148 total aligned reads displayed.

TGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCCACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTATTCTGCGGCG  >  NC_000913/3659008‑3659277

tgtgTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTa                                                                                                                                    <  1:1717734/140‑1 (MQ=255)

  tgtATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTAtt                                                                                                                                  >  2:154859/1‑139 (MQ=255)

    tATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGcc                                                                                                                                >  2:1956095/1‑140 (MQ=255)

     aTCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCg                                                                                                                               <  1:1632742/140‑1 (MQ=255)

     aTCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCg                                                                                                                               <  1:352712/140‑1 (MQ=255)

     aTCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCg                                                                                                                               <  2:1473582/140‑1 (MQ=255)

     aTCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCg                                                                                                                               <  1:458839/140‑1 (MQ=255)

      tCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGc                                                                                                                              >  1:1078482/1‑140 (MQ=255)

       ccaccaGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCt                                                                                                                             >  2:594114/1‑140 (MQ=255)

       ccaccaGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCt                                                                                                                             >  2:827654/1‑140 (MQ=255)

        caccaGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTg                                                                                                                            <  1:375369/140‑1 (MQ=255)

        caccaGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTg                                                                                                                            <  2:1230848/140‑1 (MQ=255)

         accaGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTgt                                                                                                                           >  2:689239/1‑139 (MQ=255)

          ccaGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGt                                                                                                                          <  1:628712/140‑1 (MQ=255)

          ccaGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGt                                                                                                                          >  2:873645/1‑140 (MQ=255)

           caGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTc                                                                                                                         >  2:1451840/1‑140 (MQ=255)

            aGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCt                                                                                                                        >  2:1983448/1‑140 (MQ=255)

              tAGAACCCTTCGTTGCCCGAATGTTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGt                                                                                                                      >  2:1365228/1‑140 (MQ=255)

              tAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGt                                                                                                                      >  1:2118251/1‑140 (MQ=255)

               aGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTa                                                                                                                     <  1:1548358/140‑1 (MQ=255)

                gAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTaa                                                                                                                    <  2:1186777/140‑1 (MQ=255)

                gAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTaa                                                                                                                    <  1:585922/140‑1 (MQ=255)

                 aaCCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTgg                                                                                                                           >  1:1737088/1‑132 (MQ=255)

                 aaCCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTaaa                                                                                                                   <  1:546156/140‑1 (MQ=255)

                 aaCCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTaaa                                                                                                                   >  1:154151/1‑140 (MQ=255)

                 aaCCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTaaa                                                                                                                   <  1:1983448/140‑1 (MQ=255)

                 aaCCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTaaa                                                                                                                   <  2:1833817/140‑1 (MQ=255)

                 aaCCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTATGTaaa                                                                                                                   <  1:1451840/140‑1 (MQ=255)

                   cccTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATc                                                                                                                 <  2:1203253/140‑1 (MQ=255)

                    ccTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATcc                                                                                                                >  2:907752/1‑140 (MQ=255)

                     cTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATccc                                                                                                               >  2:1900143/1‑140 (MQ=255)

                      ttCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCt                                                                                                              >  1:1302459/1‑140 (MQ=255)

                      ttCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCt                                                                                                              <  2:173781/140‑1 (MQ=255)

                        cGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTct                                                                                                            >  2:565633/1‑139 (MQ=255)

                        cGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCa                                                                                                            >  1:13551/1‑140 (MQ=255)

                        cGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCa                                                                                                            >  2:750640/1‑140 (MQ=255)

                         gTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCat                                                                                                           <  1:619533/140‑1 (MQ=255)

                             cccGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATAtct                                                                                                       >  1:267778/1‑140 (MQ=255)

                             cccGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATAtct                                                                                                       <  2:599018/140‑1 (MQ=255)

                                 aaTGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTctcc                                                                                                   >  1:1826595/1‑140 (MQ=255)

                                        gCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTcgcgcg                                                                                            <  2:154151/140‑1 (MQ=255)

                                        gCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTcgcgcg                                                                                            <  1:1956095/140‑1 (MQ=255)

                                        gCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTcgcgcg                                                                                            <  1:104842/140‑1 (MQ=255)

                                        gCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTcgcgcg                                                                                            <  1:2005112/140‑1 (MQ=255)

                                        gCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTcgcgcg                                                                                            >  2:410626/1‑140 (MQ=255)

                                          aGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCa                                                                                          <  1:594114/140‑1 (MQ=255)

                                                 gTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTaaa                                                                                   <  2:2143003/140‑1 (MQ=255)

                                                  ttGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAg                                                                                  <  1:689239/140‑1 (MQ=255)

                                                  ttGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAg                                                                                  <  1:725234/140‑1 (MQ=255)

                                                  ttGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAg                                                                                  >  2:1248021/1‑140 (MQ=255)

                                                  ttGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAg                                                                                  <  2:1045069/140‑1 (MQ=255)

                                                      cAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAAcc                                                                              >  2:1758947/1‑140 (MQ=255)

                                                         aaCGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGtt                                                                           >  2:788907/1‑140 (MQ=255)

                                                         aaCGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGtt                                                                           >  2:1028027/1‑140 (MQ=255)

                                                          aCGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTa                                                                          >  1:1570190/1‑140 (MQ=255)

                                                              cAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCAGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTtc                                                                      >  2:1619770/1‑140 (MQ=255)

                                                                          tGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTc                                                          <  1:1619770/140‑1 (MQ=255)

                                                                          tGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTc                                                          <  1:827654/140‑1 (MQ=255)

                                                                          tGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTc                                                          <  1:1248021/140‑1 (MQ=255)

                                                                          tGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTc                                                          <  2:267778/140‑1 (MQ=255)

                                                                           gTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCa                                                         >  1:206173/1‑140 (MQ=255)

                                                                           gTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCa                                                         >  2:1021303/1‑140 (MQ=255)

                                                                            tCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAg                                                        >  1:357293/1‑140 (MQ=255)

                                                                            tCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCATCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAg                                                        >  1:1410680/1‑140 (MQ=255)

                                                                             cGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAgg                                                       <  1:565633/140‑1 (MQ=255)

                                                                               ttGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGa                                                     <  1:1758947/140‑1 (MQ=255)

                                                                               ttGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGa                                                     <  2:1826595/140‑1 (MQ=255)

                                                                                    cTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTaaa                                                <  2:1081912/140‑1 (MQ=255)

                                                                                      cACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGTACTAAAAt                                              >  2:1693276/1‑140 (MQ=255)

                                                                                      cACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAAt                                              >  1:1291497/1‑140 (MQ=255)

                                                                                      cACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAAt                                              >  1:335825/1‑140 (MQ=255)

                                                                                         cATGTCGATCACTGTGCCTTTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGaa                                           <  1:1792870/140‑1 (MQ=255)

                                                                                         cATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGaa                                           <  2:1040908/140‑1 (MQ=255)

                                                                                         cATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGaa                                           <  1:1940542/140‑1 (MQ=255)

                                                                                         cATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGaa                                           <  1:2044317/140‑1 (MQ=255)

                                                                                            gTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACag                                        <  2:445840/140‑1 (MQ=255)

                                                                                                 tCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACagaagaa                                   <  1:410626/140‑1 (MQ=255)

                                                                                                 tCACTGTGCCTGTACCACCCCGTACTGGCTGTCAACCCCGCTAAGCCGCTCGTCTGTAAATAAATCATATGTCTCCTCGCGCGCAATTTAAAGAACCGTCATTTCTCAATAAAGGTCAGGGACTGAAATGAACagaagaa                                   <  2:391040/140‑1 (MQ=255)

                                                                                                   aCTGTGCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACagaagaaga                                 <  2:2116773/140‑1 (MQ=255)

                                                                                                     tgtgCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGaaa                               <  1:450714/140‑1 (MQ=255)

                                                                                                     tgtgCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGaaa                               <  1:309001/140‑1 (MQ=255)

                                                                                                     tgtgCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGaaa                               <  2:335825/140‑1 (MQ=255)

                                                                                                     tgtgCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGaaa                               <  2:357293/140‑1 (MQ=255)

                                                                                                       tgCCTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGc                             <  2:2118251/140‑1 (MQ=255)

                                                                                                         ccTGTATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTg                           >  2:1017595/1‑140 (MQ=255)

                                                                                                             tATCCTACCTTACTGACTGACAACCCAACTATGCCGCTGGTCTGAAAACCCCTCATCTCTCTCCGCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTGTCAGGCACTAAAATGATCAGAAGAAGAAAGCTGTTat                       >  2:1198251/1‑139 (MQ=255)

                                                                                                             tATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTaa                       <  2:341576/140‑1 (MQ=255)

                                                                                                             tATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTaa                       >  1:763369/1‑140 (MQ=255)

                                                                                                             tATCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTaa                       >  1:1849755/1‑140 (MQ=255)

                                                                                                              aTCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAAt                      >  1:1074567/1‑140 (MQ=255)

                                                                                                               tCCCACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATa                     >  2:493306/1‑140 (MQ=255)

                                                                                                                cccACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATAc                    <  1:1900143/140‑1 (MQ=255)

                                                                                                                accACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATAc                    <  2:13551/139‑1 (MQ=255)

                                                                                                                  cACCTTACTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCg                  <  1:1028027/140‑1 (MQ=255)

                                                                                                                        aCTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCgttgttt            >  2:381819/1‑139 (MQ=255)

                                                                                                                         cTGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTAt           >  2:389520/1‑140 (MQ=255)

                                                                                                                          tGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTAtt          >  2:1641804/1‑140 (MQ=255)

                                                                                                                          tGGCTGACAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTAtt          >  1:1585139/1‑140 (MQ=255)

                                                                                                                                 cAACCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTATTCTgcggc   <  1:1017595/140‑1 (MQ=255)

                                                                                                                                  aaCCCAACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTATTCTgcggcg  <  2:1706814/140‑1 (MQ=255)

TGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCACCATGTCGATCACTGTGCCTGTATCCCACCTTACTGGCTGACAACCCCACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTCAAGAATTTTCAGGGACTAAAATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTATTCTGCGGCG  >  NC_000913/3659008‑3659277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A