Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,964,684 G→T A316D (GCC→GAC)  rhlB ← ATP‑dependent RNA helicase RhlB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,6840GT100.0% 448.7 / NA 131A316D (GCC→GAC) rhlBATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (76/55);  total (76/55)

Only 100 of 133 total aligned reads displayed.

CACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCT  >  NC_000913/3964547‑3964819
                                                                                                                                         |                                                                                                                                       
cacaCGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAAACACGCGCGTCa                                                                                                                                       >  2:946507/1‑140 (MQ=255)
 acacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCaa                                                                                                                                      >  2:584858/1‑140 (MQ=255)
   acGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACg                                                                                                                                    >  2:1759134/1‑140 (MQ=255)
     gCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTc                                                                                                                                  >  2:144093/1‑140 (MQ=255)
      ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCg                                                                                                                                 >  1:304690/1‑140 (MQ=255)
      ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCg                                                                                                                                 >  1:1149946/1‑140 (MQ=255)
      ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCg                                                                                                                                 >  2:1791865/1‑140 (MQ=255)
      ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCg                                                                                                                                 >  2:1565964/1‑140 (MQ=255)
      ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCg                                                                                                                                 >  1:1306216/1‑140 (MQ=255)
      ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCg                                                                                                                                 >  1:1728805/1‑140 (MQ=255)
      ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCg                                                                                                                                 >  2:1636328/1‑140 (MQ=255)
      ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCg                                                                                                                                 >  1:1709980/1‑140 (MQ=255)
        aGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGt                                                                                                                               >  1:947776/1‑140 (MQ=255)
        aGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGt                                                                                                                               >  2:8208/1‑140 (MQ=255)
         ggCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTg                                                                                                                              <  1:1936006/140‑1 (MQ=255)
          gCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTgg                                                                                                                             >  2:514879/1‑140 (MQ=255)
          gCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTgg                                                                                                                             >  1:222680/1‑140 (MQ=255)
          gCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGCGAACGTAATCTTCACAGCCATCGGGTAAATCGTAGTTAAATACGTTCGTCACTGCCGGAATATGCAAACCACGCGCGTCATCGTCGATgg                                                                                                                             >  2:1542083/1‑140 (MQ=255)
           cTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTACATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGc                                                                                                                            <  2:1288327/140‑1 (MQ=255)
           cTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGc                                                                                                                            <  2:2113649/140‑1 (MQ=255)
             gATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCaa                                                                                                                          <  1:584858/140‑1 (MQ=255)
             gATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCaa                                                                                                                          <  1:1791865/140‑1 (MQ=255)
               tAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAAcc                                                                                                                        <  1:659713/140‑1 (MQ=255)
               tAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAAcc                                                                                                                        <  2:215884/140‑1 (MQ=255)
                  agTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGa                                                                                                                     <  2:61353/140‑1 (MQ=255)
                  agTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGa                                                                                                                     <  1:464835/140‑1 (MQ=255)
                    tGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAat                                                                                                                   >  2:24676/1‑140 (MQ=255)
                    tGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAat                                                                                                                   >  1:1185142/1‑140 (MQ=255)
                      aCCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAatat                                                                                                                 <  1:1759134/140‑1 (MQ=255)
                               gcCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGc                                                                                                        <  2:1936798/140‑1 (MQ=255)
                               gcCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGc                                                                                                        <  2:1065335/140‑1 (MQ=255)
                                ccTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGcc                                                                                                       <  1:8208/140‑1 (MQ=255)
                                 cTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCa                                                                                                      <  2:466457/140‑1 (MQ=255)
                                    cgcgACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCCACCAGAATATCCAGATCGCCACgg                                                                                                   >  2:1065755/1‑140 (MQ=255)
                                     gcgACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACggg                                                                                                  >  1:1027677/1‑140 (MQ=255)
                                       gACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTa                                                                                                <  1:1265762/140‑1 (MQ=255)
                                         ccTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTaaa                                                                                              <  1:1636328/140‑1 (MQ=255)
                                         ccTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTaaa                                                                                              <  1:1565964/140‑1 (MQ=255)
                                         acTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTaaa                                                                                              <  2:1728805/139‑1 (MQ=255)
                                              aCGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATtcat                                                                                         <  1:896785/140‑1 (MQ=255)
                                                 aCCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCaa                                                                                      >  1:224385/1‑140 (MQ=255)
                                                      tACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATa                                                                                 >  2:1203477/1‑140 (MQ=255)
                                                          gTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCa                                                                             <  1:40420/140‑1 (MQ=255)
                                                           tGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAg                                                                            >  1:1066561/1‑140 (MQ=255)
                                                             aaCGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGAc                                                                          >  2:82529/1‑140 (MQ=255)
                                                                   aTCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttttt                                                                    >  2:1037448/1‑140 (MQ=255)
                                                                   aTCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttttt                                                                    >  1:2105548/1‑140 (MQ=255)
                                                                    tCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGtttttt                                                                   <  1:514879/140‑1 (MQ=255)
                                                                    tCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGtttttt                                                                   >  1:824843/1‑140 (MQ=255)
                                                                        cacaGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTgc                                                               <  2:947776/140‑1 (MQ=255)
                                                                             tCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGAc                                                          >  1:1080877/1‑140 (MQ=255)
                                                                               aTCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACAt                                                        >  2:1025297/1‑140 (MQ=255)
                                                                               aTCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACAt                                                        <  2:1149946/140‑1 (MQ=255)
                                                                                tCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATc                                                       >  1:1185037/1‑140 (MQ=255)
                                                                                tCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATc                                                       >  1:317853/1‑140 (MQ=255)
                                                                                 cGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCg                                                      <  2:2105548/140‑1 (MQ=255)
                                                                                   ggTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGcc                                                    <  2:1306216/140‑1 (MQ=255)
                                                                                    gTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCAACAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCt                                                   <  2:1880149/140‑1 (MQ=255)
                                                                                       aaTCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGtt                                                >  2:1805576/1‑139 (MQ=255)
                                                                                       aaTCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTc                                                >  2:801019/1‑140 (MQ=255)
                                                                                       aaTCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTc                                                >  1:767404/1‑140 (MQ=255)
                                                                                             aGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATaaa                                          >  2:512825/1‑140 (MQ=255)
                                                                                             aGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATaaa                                          >  2:1260214/1‑140 (MQ=255)
                                                                                              gTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAAc                                         >  1:1821654/1‑140 (MQ=255)
                                                                                               ttAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAAcc                                        <  2:304690/140‑1 (MQ=255)
                                                                                                  aaGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGac                                     <  2:935723/140‑1 (MQ=255)
                                                                                                   aGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGaca                                    >  1:867589/1‑140 (MQ=255)
                                                                                                       gTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGa                                 <  2:329930/139‑1 (MQ=255)
                                                                                                       gTGCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGAt                                >  1:1414983/1‑140 (MQ=255)
                                                                                                         gCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                              >  2:157629/1‑140 (MQ=255)
                                                                                                         gCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                              >  1:1278830/1‑140 (MQ=255)
                                                                                                         gCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                              >  1:1236727/1‑140 (MQ=255)
                                                                                                         gCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                              >  2:821265/1‑140 (MQ=255)
                                                                                                         gCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                              >  2:775180/1‑140 (MQ=255)
                                                                                                         gCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                              >  1:1982558/1‑140 (MQ=255)
                                                                                                         gCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                              >  2:1942772/1‑140 (MQ=255)
                                                                                                         gCGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGCCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                              >  1:965111/1‑140 (MQ=255)
                                                                                                          cGTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGAc                             <  1:1835787/140‑1 (MQ=255)
                                                                                                           gTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGAcc                            <  2:224385/140‑1 (MQ=255)
                                                                                                           gTCACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGAcc                            <  1:1842092/140‑1 (MQ=255)
                                                                                                             cACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCAt                          <  1:1037448/140‑1 (MQ=255)
                                                                                                             cACTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCAt                          <  2:341808/140‑1 (MQ=255)
                                                                                                              aCTGCCGGAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATc                         >  1:307660/1‑140 (MQ=255)
                                                                                                                    ggAATATGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCtgctgc                   <  2:1412197/140‑1 (MQ=255)
                                                                                                                       atatGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAg                >  2:1739969/1‑140 (MQ=255)
                                                                                                                         atGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGt              <  1:558464/140‑1 (MQ=255)
                                                                                                                         atGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGt              <  1:1473688/140‑1 (MQ=255)
                                                                                                                         atGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGt              <  1:1892080/140‑1 (MQ=255)
                                                                                                                         atGCAAACCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGt              <  2:767404/140‑1 (MQ=255)
                                                                                                                               aCCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGcccc        <  1:821265/140‑1 (MQ=255)
                                                                                                                               aCCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGcccc        >  1:886905/1‑140 (MQ=255)
                                                                                                                               aCCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGcccc        >  1:1306457/1‑140 (MQ=255)
                                                                                                                                ccACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCa       >  1:1474949/1‑140 (MQ=255)
                                                                                                                                   cgcgcgTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAg                >  2:60553/1‑128 (MQ=255)
                                                                                                                                    gcgcgTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCTCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtc   >  2:2135099/1‑140 (MQ=255)
                                                                                                                                    gcgcgTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtc   >  1:567615/1‑140 (MQ=255)
                                                                                                                                    gcgcgTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtc   >  1:1952486/1‑140 (MQ=255)
                                                                                                                                    gcgcgTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtc   >  1:747111/1‑140 (MQ=255)
                                                                                                                                     cgcgTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtct  <  2:1982558/140‑1 (MQ=255)
                                                                                                                                     cgcgTCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtct  <  2:1185037/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCT  >  NC_000913/3964547‑3964819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A