Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,061,216	C→A	P18T (CCT→ACT)	yihM →	putative TIM barrel domain‑containing protein YihM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,061,216	0	C	A	99.1%	385.2 / ‑6.4	113	P18T (CCT→ACT)	yihM	putative TIM barrel domain‑containing protein YihM
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (69/43); total (69/44)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGTTTTTC  >  NC_000913/4061079‑4061352

cGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTAct                                                                                                                                        <  2:942327/140‑1 (MQ=255)

cGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTAct                                                                                                                                        <  1:1969829/140‑1 (MQ=255)

cGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTAct                                                                                                                                        <  1:770285/140‑1 (MQ=255)

cGACCAGCAAGACAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTAct                                                                                                                                        <  2:393962/140‑1 (MQ=255)

  aCCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTActct                                                                                                                                      <  1:1127212/140‑1 (MQ=255)

  aCCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTActct                                                                                                                                      <  1:1754785/140‑1 (MQ=255)

   acAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACactcttactctt                                                                                                                                     <  2:323697/139‑1 (MQ=255)

    aaGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAGCGTGTCGACACTCTTACTCttt                                                                                                                                    <  2:655979/139‑1 (MQ=255)

         aGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCtttattta                                                                                                                               <  2:776568/140‑1 (MQ=255)

          ggCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTAc                                                                                                                              <  2:1779670/140‑1 (MQ=255)

           gCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACa                                                                                                                             <  1:703531/140‑1 (MQ=255)

                 ttGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTa                                                                                                                       <  2:1377720/140‑1 (MQ=255)

                 ttGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTa                                                                                                                       <  2:19400/140‑1 (MQ=255)

                 ttGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTa                                                                                                                       >  1:1157284/1‑140 (MQ=255)

                    tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACTCACTTACTCTTTATTTACATGCTTATGc                                                                                                                    >  1:522993/1‑140 (MQ=255)

                    tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGc                                                                                                                    >  1:2044916/1‑140 (MQ=255)

                     ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGcc                                                                                                                   >  1:1862688/1‑140 (MQ=255)

                     ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGcc                                                                                                                   >  1:239819/1‑140 (MQ=255)

                     ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGcc                                                                                                                   <  2:930632/140‑1 (MQ=255)

                       aaaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCtt                                                                                                                 <  1:1614597/140‑1 (MQ=255)

                              tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCt                                                                                                                  >  2:329840/1‑132 (MQ=255)

                              tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTa                                                                                                          >  1:749501/1‑140 (MQ=255)

                                 tGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAat                                                                                                       >  1:1427394/1‑140 (MQ=255)

                                  gAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAata                                                                                                      >  2:2119670/1‑140 (MQ=255)

                                     tatTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGc                                                                                                   >  1:703628/1‑140 (MQ=255)

                                        tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAata                                                                                                      <  1:1753418/134‑1 (MQ=255)

                                        tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGc                                                                                                >  2:53755/1‑140 (MQ=255)

                                         ggCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt                                                                                               <  1:398480/140‑1 (MQ=255)

                                          gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTg                                                                                              >  1:441424/1‑140 (MQ=255)

                                             aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGaa                                                                                           >  1:768303/1‑140 (MQ=255)

                                             aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGaa                                                                                           >  2:1323857/1‑140 (MQ=255)

                                             aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGaa                                                                                           >  1:1125376/1‑140 (MQ=255)

                                             aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGaa                                                                                           <  2:1427394/140‑1 (MQ=255)

                                             aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGaa                                                                                           <  1:388827/140‑1 (MQ=255)

                                                         aGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCct                                                                               <  1:1157535/140‑1 (MQ=255)

                                                             cTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgctg                                                                           <  2:522993/140‑1 (MQ=255)

                                                                    ttAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACt                                                                    >  2:1526510/1‑140 (MQ=255)

                                                                          tGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGatat                                                              >  2:19208/1‑140 (MQ=255)

                                                                          tGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGatat                                                              >  1:816112/1‑140 (MQ=255)

                                                                          tGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGatat                                                              >  2:1685424/1‑140 (MQ=255)

                                                                          tGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGatat                                                              >  1:502866/1‑140 (MQ=255)

                                                                           gTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAATAGTGTTGCCTGCTGATTTACTTGATATc                                                             >  2:2017285/1‑140 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATcc                                                            >  2:655081/1‑139 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCg                                                            >  1:560238/1‑140 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCg                                                            <  1:1323857/140‑1 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCg                                                            >  1:1581584/1‑140 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCg                                                            >  1:1848961/1‑140 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCg                                                            >  2:1554664/1‑140 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCg                                                            <  2:1157284/140‑1 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCCGGAAAGAGTGTTGCCTGCTGTTTTACTTGATATCg                                                            >  1:928074/1‑140 (MQ=255)

                                                                            tAGGTGTTTTATGGTGACAATAAATAACGCAAGAA‑‑ATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCa                                                          >  2:930789/1‑140 (MQ=255)

                                                                             aGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGc                                                           >  1:105630/1‑140 (MQ=255)

                                                                               gtgtTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCaa                                                         >  2:1460171/1‑140 (MQ=255)

                                                                                 gtTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGt                                                       >  1:1600874/1‑140 (MQ=255)

                                                                                  ttttATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTg                                                      >  1:289123/1‑140 (MQ=255)

                                                                                   tttATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGa                                                     >  1:207152/1‑140 (MQ=255)

                                                                                   tttATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGa                                                     >  2:273587/1‑140 (MQ=255)

                                                                                    ttATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGaa                                                    >  2:213987/1‑140 (MQ=255)

                                                                                     tATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGaaa                                                   <  1:244764/140‑1 (MQ=255)

                                                                                      aTGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGaaaa                                                  <  1:1526510/140‑1 (MQ=255)

                                                                                          tGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAaataat                                              <  1:1271229/140‑1 (MQ=255)

                                                                                            aCAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCt                                            >  2:1165665/1‑140 (MQ=255)

                                                                                            aCAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCt                                            >  2:90740/1‑140 (MQ=255)

                                                                                            aCAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCt                                            >  2:792471/1‑140 (MQ=255)

                                                                                            aCAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTCACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCt                                            >  1:1165808/1‑140 (MQ=255)

                                                                                                   ataaCGCAAGAAAGATTCTACAACGTGTCGACACTCTCACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGc                                     <  2:1165808/140‑1 (MQ=255)

                                                                                                     aaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGt                                   <  2:1848961/140‑1 (MQ=255)

                                                                                                     aaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGt                                   <  1:90740/140‑1 (MQ=255)

                                                                                                      aCGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTc                                  >  1:1222535/1‑140 (MQ=255)

                                                                                                      aCGCAAGAAAGATTCTACAACGGGTCGACACTCTTACTCTTTATGTACATGCTTATGCCTTTCATTGAAATATGCGGCTGGAAAGAATGGTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTc                                  >  2:1434567/1‑140 (MQ=255)

                                                                                                        gCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCaa                                >  1:1473246/1‑140 (MQ=255)

                                                                                                        gCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCaa                                >  2:258802/1‑140 (MQ=255)

                                                                                                        gCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCaa                                >  1:476914/1‑140 (MQ=255)

                                                                                                        gCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCaa                                >  2:1762212/1‑140 (MQ=255)

                                                                                                        gCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCaa                                >  2:1735862/1‑140 (MQ=255)

                                                                                                        gCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCaa                                >  1:777309/1‑140 (MQ=255)

                                                                                                        gCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCaa                                >  2:1526099/1‑140 (MQ=255)

                                                                                                            gaaagaTTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATc                            >  2:2070344/1‑140 (MQ=255)

                                                                                                            gaaagaTTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATc                            >  1:1357018/1‑140 (MQ=255)

                                                                                                            gaaagaTTCTACAACGGGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATc                            >  1:1582891/1‑140 (MQ=255)

                                                                                                             aaagaTTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATcc                           <  1:19208/140‑1 (MQ=255)

                                                                                                                gaTTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATg                        <  2:105630/140‑1 (MQ=255)

                                                                                                                 aTTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGt                       >  2:23453/1‑140 (MQ=255)

                                                                                                                      aCAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTtctct                  >  2:176917/1‑139 (MQ=255)

                                                                                                                           gtgtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGc             >  2:1489301/1‑140 (MQ=255)

                                                                                                                            tgtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCg            >  1:1621373/1‑140 (MQ=255)

                                                                                                                            tgtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCg            >  1:255805/1‑140 (MQ=255)

                                                                                                                            tgtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCg            >  1:629264/1‑140 (MQ=255)

                                                                                                                            tgtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCg            >  1:665712/1‑140 (MQ=255)

                                                                                                                            tgtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCg            >  2:312885/1‑140 (MQ=255)

                                                                                                                            tgtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCg            >  2:2058870/1‑140 (MQ=255)

                                                                                                                             gtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGa           <  2:703628/140‑1 (MQ=255)

                                                                                                                             gtCGACACTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGa           <  1:763012/140‑1 (MQ=255)

                                                                                                                                  cacTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGtt      <  2:2107849/140‑1 (MQ=255)

                                                                                                                                  cacTCTTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGtt      <  1:2119670/140‑1 (MQ=255)

                                                                                                                                  aactcttactcttTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGtt      <  2:1645742/139‑1 (MQ=255)

                                                                                                                                  aactcttactcttTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGtt      <  2:749501/139‑1 (MQ=255)

                                                                                                                                     tctTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGttttt   >  1:686667/1‑140 (MQ=255)

                                                                                                                                      ctTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGTTTTtc  <  2:1125376/140‑1 (MQ=255)

                                                                                                                                      ctTACTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGTTTTtc  <  1:157401/140‑1 (MQ=255)

CGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGTTCTCGATGGCGAGCGTTTTTC  >  NC_000913/4061079‑4061352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A