Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,225,958	G→T	G711W (GGG→TGG)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,225,958	0	G	T	98.2%	364.3 / ‑0.2	112	G711W (GGG→TGG)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (66/44); total (67/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 117 total aligned reads displayed.

CTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAAGGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATCGCCACCGTGAAG  >  NC_000913/4225822‑4226092

cTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGa                                                                                                                                     >  1:526442/1‑140 (MQ=255)

  cGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGaaa                                                                                                                                   <  1:2056810/140‑1 (MQ=255)

   gCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGaaaa                                                                                                                                  >  2:1911959/1‑140 (MQ=255)

     tGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATg                                                                                                                                >  1:1026007/1‑140 (MQ=255)

      ggTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGt                                                                                                                               >  1:2137576/1‑140 (MQ=255)

       gTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGtt                                                                                                                              <  2:602112/140‑1 (MQ=255)

        tCAAAGGCATTCCCGAGTTTATCGAGCAGGATCCCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTc                                                                                                                             <  1:1020675/140‑1 (MQ=255)

        tCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTc                                                                                                                             <  2:1026007/140‑1 (MQ=255)

           aaGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTg                                                                                                                          >  1:1292976/1‑140 (MQ=255)

                 ttACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAg                                                                                                                    >  2:1419115/1‑140 (MQ=255)

                  tACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAgg                                                                                                                   >  1:302731/1‑140 (MQ=255)

                   aCCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAggt                                                                                                                  <  2:669071/140‑1 (MQ=255)

                       aGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTc                                                                                                              >  1:808236/1‑140 (MQ=255)

                       aGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTc                                                                                                              >  1:205791/1‑140 (MQ=255)

                       aGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGAGGTc                                                                                                              >  1:897982/1‑140 (MQ=255)

                         tttATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGATGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCaa                                                                                                            >  1:2117620/1‑140 (MQ=255)

                         tttATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCaa                                                                                                            >  2:1523026/1‑140 (MQ=255)

                         tttATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCaa                                                                                                            >  1:574518/1‑140 (MQ=255)

                           tATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGATCAAAt                                                                                                          >  2:2050603/1‑140 (MQ=255)

                                  cAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGgcgcg                                                                                                   >  2:1174551/1‑140 (MQ=255)

                                  cAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGgcgcg                                                                                                   <  1:56538/140‑1 (MQ=255)

                                       tACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCa                                                                                              >  1:1815942/1‑140 (MQ=255)

                                       aaCCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCa                                                                                              <  1:1364272/139‑1 (MQ=255)

                                          cGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGa                                                                                           >  2:1210406/1‑140 (MQ=255)

                                          cGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGa                                                                                           >  2:192337/1‑140 (MQ=255)

                                             agaagCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAAc                                                                                        <  2:1574770/140‑1 (MQ=255)

                                               aagCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAg                                                                                      >  1:512422/1‑140 (MQ=255)

                                               aagCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAg                                                                                      >  2:651304/1‑140 (MQ=255)

                                               aagCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAg                                                                                      >  2:1898061/1‑140 (MQ=255)

                                               aagCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAg                                                                                      >  1:1658597/1‑140 (MQ=255)

                                                agCCCGCCAGCAGGCTAGGCGCCCGATTGAAGTGATTGAAGGCCGGTTGATGGTCGACATGATTGTGGTCGGCGACCTGTTTGGCGAGTGGAAAATTTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAATCAgg                                                                                     <  2:1142458/140‑1 (MQ=255)

                                                agCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAgg                                                                                     <  2:2073138/140‑1 (MQ=255)

                                                 gCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGc                                                                                    >  1:488939/1‑140 (MQ=255)

                                                 acccGCCGGCAGGCTACGCGCGCGATTGAAGTGATTGAAGACCCGTTGATGGACGGAATGAATATGGTCGGCGACCTGTTTGGCGATGGGAAAGTGTTCCTGCCACAGGTGGTCAAATCGGCACGCGTCATGAAACAGGc                                                                                    <  2:574518/139‑1 (MQ=255)

                                                  cccGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCg                                                                                   <  2:512422/140‑1 (MQ=255)

                                                  cccGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCg                                                                                   <  1:1306911/140‑1 (MQ=255)

                                                  cccGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCg                                                                                   <  1:777661/140‑1 (MQ=255)

                                                    cGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGt                                                                                 >  1:369372/1‑140 (MQ=255)

                                                      ccAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTc                                                                                               >  1:1121651/1‑124 (MQ=255)

                                                      ccAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTc                                                                                               <  2:1121651/124‑1 (MQ=255)

                                                      ccAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAAGGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCgg                                                                                  >  2:1302743/1‑137 (MQ=255)

                                                      ccAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAAGGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCgg                                                                                  <  1:1302743/137‑1 (MQ=255)

                                                       cagcagGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGATGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGc                                                                              <  2:2117620/140‑1 (MQ=255)

                                                             gCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCt                                                                        >  1:1142790/1‑140 (MQ=255)

                                                                aCGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGa                                                                     <  1:1971412/140‑1 (MQ=255)

                                                                aCGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGa                                                                     <  1:35479/140‑1 (MQ=255)

                                                                 cgcgCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGaa                                                                    <  1:990071/140‑1 (MQ=255)

                                                                   cgcCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAAcc                                                                  >  1:1084132/1‑140 (MQ=255)

                                                                    gcCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCg                                                                 >  1:1231791/1‑140 (MQ=255)

                                                                       cGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGttt                                                              >  1:671579/1‑140 (MQ=255)

                                                                       cGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGttt                                                              >  2:755113/1‑140 (MQ=255)

                                                                         aTTGAAGTGATTGAAGGCCCGTTGAAGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTAt                                                            >  2:1951745/1‑140 (MQ=255)

                                                                          ttGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTAtt                                                           <  2:808236/140‑1 (MQ=255)

                                                                          aggAAGTGATTGAAGGACCGTTGATGAACGGCATGAAGGTGGTCGGGGACCTGTTTGGGGAATGGAAAATTTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTAtt                                                           <  2:488939/138‑1 (MQ=255)

                                                                            gAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGa                                                         <  2:369372/140‑1 (MQ=255)

                                                                               gTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGc                                                      <  2:2137576/140‑1 (MQ=255)

                                                                               gTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGc                                                      <  2:526442/140‑1 (MQ=255)

                                                                                tGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGcc                                                     >  1:899727/1‑140 (MQ=255)

                                                                                  aTTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAg                                                   >  2:487355/1‑140 (MQ=255)

                                                                                    tGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCa                                                 >  2:1522147/1‑140 (MQ=255)

                                                                                       aGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAag                                              >  1:988875/1‑140 (MQ=255)

                                                                                         gCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAagag                                            <  2:671579/140‑1 (MQ=255)

                                                                                          cccGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGc                                           <  1:651304/140‑1 (MQ=255)

                                                                                          cccGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGc                                           >  1:1737910/1‑140 (MQ=255)

                                                                                                   ggACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCaaaa                                  <  1:1174551/140‑1 (MQ=255)

                                                                                                      cGGCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCT‑CCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCaa                              >  1:170495/1‑140 (MQ=255)

                                                                                                       ggCATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCaa                              >  2:1764295/1‑140 (MQ=255)

                                                                                                         cATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACg                            >  1:645933/1‑140 (MQ=255)

                                                                                                         cATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACg                            >  1:645467/1‑140 (MQ=255)

                                                                                                         cATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACg                            >  1:581807/1‑140 (MQ=255)

                                                                                                         cATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACg                            >  1:366424/1‑140 (MQ=255)

                                                                                                         cATGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTGATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACg                            >  2:1121134/1‑140 (MQ=255)

                                                                                                         aaTGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACg                            <  2:1648014/139‑1 (MQ=255)

                                                                                                          aTGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACgg                           <  2:581807/140‑1 (MQ=255)

                                                                                                          aTGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACgg                           <  2:1292976/140‑1 (MQ=255)

                                                                                                           tGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACgg                           <  2:1603084/139‑1 (MQ=255)

                                                                                                           tGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACgg                           >  1:1603084/1‑139 (MQ=255)

                                                                                                           tGAATGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGc                          <  2:1142790/140‑1 (MQ=255)

                                                                                                             aaTGTGGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCaa                        <  1:1911959/140‑1 (MQ=255)

                                                                                                               tgtgGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGa                      <  1:1636840/140‑1 (MQ=255)

                                                                                                               tggggTCGGCTACTTGTTTGGCAAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCATTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGa                      <  2:1694815/137‑1 (MQ=255)

                                                                                                                 tgGTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATg                    >  2:2008195/1‑140 (MQ=255)

                                                                                                                   gTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTATCTCTAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATggg                  >  1:121552/1‑139 (MQ=255)

                                                                                                                   gTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGt                  >  2:1391884/1‑140 (MQ=255)

                                                                                                                   gTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGt                  >  1:2025293/1‑140 (MQ=255)

                                                                                                                   gTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGt                  >  1:525810/1‑140 (MQ=255)

                                                                                                                   gTCGGCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGt                  >  2:812684/1‑140 (MQ=255)

                                                                                                                      ggCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGAt               <  1:833974/140‑1 (MQ=255)

                                                                                                                      ggCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGAt               <  1:812684/140‑1 (MQ=255)

                                                                                                                      ggCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGAt               <  2:899727/140‑1 (MQ=255)

                                                                                                                      ggCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGAt               <  2:205791/140‑1 (MQ=255)

                                                                                                                      ggCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGAt               <  2:988875/140‑1 (MQ=255)

                                                                                                                      ggCAACCTGCTAGGCGAATGGAAAATGTTCCTGCTACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAAAGGCAAGATGGTGAt               <  2:897982/140‑1 (MQ=255)

                                                                                                                       gCGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATc              >  2:2120082/1‑140 (MQ=255)

                                                                                                                        cGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATcc             >  1:746986/1‑139 (MQ=255)

                                                                                                                        cGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATCg             >  2:317683/1‑140 (MQ=255)

                                                                                                                        cGACCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATCg             >  2:2132533/1‑140 (MQ=255)

                                                                                                                          aCCTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCC‑ACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATCGCCa          <  2:1658597/140‑1 (MQ=255)

                                                                                                                           ccTGTTTGGCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATCGCCa          <  2:302731/140‑1 (MQ=255)

                                                                                                                                   gCGAATGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATCGCCACCGTGAAg  >  1:1653163/1‑140 (MQ=255)

CTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTGTTTGGCGAAGGGAAAATGTTCCTGCCACAGGTGGTCAAATCGGCGCGCGTCATGAAACAGGCGGTGGCCTACCTCGAACCGTTTATTGAAGCCAGCAAAGAGCAGGGCAAAACCAACGGCAAGATGGTGATCGCCACCGTGAAG  >  NC_000913/4225822‑4226092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A