Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F48 I1 R1 76 74.1 2532288 99.0% 2506965 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,246,357 G→T S21S (TCC→TCA)  malE ← maltose ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,246,3570GT98.6% 241.0 / ‑2.6 69S21S (TCC→TCA) malEmaltose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (25/43);  total (25/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

TCCGGATGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTAT  >  NC_000913/4246220‑4246482
                                                                                                                                         |                                                                                                                             
tCCGGATGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGa                                                                                                                             >  2:1207509/1‑140 (MQ=255)
 ccGGATGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCTGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGaa                                                                                                                            <  1:868581/140‑1 (MQ=255)
 ccGGATGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGaa                                                                                                                            <  1:472086/140‑1 (MQ=255)
    gATGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGaaaca                                                                                                                         <  2:1205632/140‑3 (MQ=255)
     aTGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAAca                                                                                                                        >  2:217230/1‑140 (MQ=255)
      tGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAAcat                                                                                                                       >  2:623600/1‑140 (MQ=255)
      tGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAAcat                                                                                                                       >  2:452423/1‑140 (MQ=255)
        cTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAAcatct                                                                                                                     >  2:1224858/1‑139 (MQ=255)
          cAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACatcatc                                                                                                                   >  1:389368/1‑140 (MQ=255)
          cAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACatcatc                                                                                                                   >  1:380682/1‑140 (MQ=255)
           aaCGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCAtcg                                                                                                                  >  2:935839/1‑140 (MQ=255)
            aCGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCAtcgt                                                                                                                 <  2:1137260/140‑1 (MQ=255)
              ggTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCAtcgtcg                                                                                                               <  1:728134/140‑1 (MQ=255)
              ggTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCAtcgtcg                                                                                                               <  2:24303/140‑1 (MQ=255)
              ggTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCAtcgtcg                                                                                                               <  1:1061065/140‑1 (MQ=255)
               gTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATcgtcgt                                                                                                              <  1:1207509/140‑1 (MQ=255)
                 gACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTa                                                                                                            <  1:852436/140‑1 (MQ=255)
                  aCTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTaa                                                                                                           <  1:452423/140‑1 (MQ=255)
                  aCTTTAATTCCGGGATCTTTCTAGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAAAGCCTATGCTGAAAACATCATCGTCGTTaa                                                                                                           >  2:65187/1‑140 (MQ=255)
                   cTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAAt                                                                                                          <  2:512851/140‑1 (MQ=255)
                       aaTTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCgg                                                                                                      >  1:1241420/1‑140 (MQ=255)
                        aTTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGa                                                                                                     <  1:217230/140‑1 (MQ=255)
                        aTTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGa                                                                                                     <  1:923326/140‑1 (MQ=255)
                        aTTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGa                                                                                                     <  1:618942/140‑1 (MQ=255)
                          tCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATa                                                                                                   <  1:623600/140‑1 (MQ=255)
                          tCCGGTATCTTACTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATTATCGTCGTTAATGCGGATa                                                                                                   <  2:773845/140‑1 (MQ=255)
                            cGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAAt                                                                                                 >  2:228614/1‑140 (MQ=255)
                            cGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAAt                                                                                                 >  1:359930/1‑140 (MQ=255)
                              gTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGc                                                                                               <  2:959723/140‑1 (MQ=255)
                              gTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGc                                                                                               >  1:382401/1‑140 (MQ=255)
                               tATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCg                                                                                              <  2:406025/140‑1 (MQ=255)
                                      ctcGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGc                                                                                       >  2:356063/1‑140 (MQ=255)
                                        cGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCGTCGTCGTTAATGTGGATAATGCGAGGATGCGt                                                                                     <  1:254404/140‑1 (MQ=255)
                                         gAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTg                                                                                    <  1:815262/140‑1 (MQ=255)
                                         gAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTg                                                                                    <  2:382401/140‑1 (MQ=255)
                                         gAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTg                                                                                    <  2:237949/140‑1 (MQ=255)
                                            tttCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCAc                                                                                 <  2:380682/140‑1 (MQ=255)
                                               cTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTg                                                                              >  1:654898/1‑140 (MQ=255)
                                                     gACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTGCCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTa                                                                        <  2:405618/140‑1 (MQ=255)
                                                     gACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTa                                                                        <  1:710183/140‑1 (MQ=255)
                                                        ttCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTAttt                                                                     >  2:1008025/1‑140 (MQ=255)
                                                        ttCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTAttt                                                                     >  1:456606/1‑140 (MQ=255)
                                                        ttCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTAttt                                                                     >  2:1149266/1‑140 (MQ=255)
                                                         tCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTAtttt                                                                    >  2:350802/1‑140 (MQ=255)
                                                          cAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTc                                                                   <  1:951806/140‑1 (MQ=255)
                                                           aGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCa                                                                  <  2:792931/140‑1 (MQ=255)
                                                                 aCCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCt                                                            >  2:718029/1‑140 (MQ=255)
                                                                  ccGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTa                                                           <  1:847291/140‑1 (MQ=255)
                                                                  ccGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTa                                                           <  1:160297/140‑1 (MQ=255)
                                                                             tttATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCttgtt                                                >  1:137988/1‑140 (MQ=255)
                                                                               tATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGTTCCttg                                                  >  2:668978/1‑136 (MQ=255)
                                                                               tATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCttg                                                  <  1:668978/136‑1 (MQ=255)
                                                                                aTCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGATAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGt                                             >  2:1038073/1‑140 (MQ=255)
                                                                                 tCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTg                                            <  1:228614/140‑1 (MQ=255)
                                                                                     cGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGt                                        >  2:436687/1‑140 (MQ=255)
                                                                                         aaTCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTc                                    >  1:378193/1‑140 (MQ=255)
                                                                                         aaTCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTc                                    >  2:597080/1‑140 (MQ=255)
                                                                                              aGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGaa                               <  2:973706/140‑1 (MQ=255)
                                                                                              aGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGaa                               <  1:347763/140‑1 (MQ=255)
                                                                                              aGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCAGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGaa                               <  1:935839/140‑1 (MQ=255)
                                                                                                   aCCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAacac                          <  2:1241420/140‑1 (MQ=255)
                                                                                                    ccAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACAcc                         <  1:436687/140‑1 (MQ=255)
                                                                                                        tttACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTaaa                     <  1:1008025/140‑1 (MQ=255)
                                                                                                        tttACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTaaa                     <  1:1149266/140‑1 (MQ=255)
                                                                                                          tACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACg                   <  1:718029/140‑1 (MQ=255)
                                                                                                          tACCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACg                   >  2:719452/1‑140 (MQ=255)
                                                                                                           aCCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACgg                  <  2:456606/140‑1 (MQ=255)
                                                                                                           aCCTTCTTCGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACgg                  <  1:65187/140‑1 (MQ=255)
                                                                                                              ttcttcGATTTTGGCGAGAGCTGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGAct               <  2:601146/140‑1 (MQ=255)
                                                                                                              ttcttcGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGAct               <  2:137988/140‑1 (MQ=255)
                                                                                                                cttcGATTTTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGActct             <  2:526906/140‑1 (MQ=255)
                                                                                                                  tcGATTGTGGCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTTCACCTGTTTTGATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAg           <  2:971375/140‑1 (MQ=255)
                                                                                                                           gCGAGAGCCGAGGCTGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTtat  <  1:1224858/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                             
TCCGGATGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGAAAACATCATCGTCGTTAATGCGGATAATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTAT  >  NC_000913/4246220‑4246482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A