Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F48 I1 R1	61	131.2	4447032	99.1%	4407008	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,277,889	G→A	E141K (GAA→AAA)	soxR →	DNA‑binding transcriptional dual regulator SoxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,277,889	0	G	A	100.0%	340.9 / NA	95	E141K (GAA→AAA)	soxR	DNA‑binding transcriptional dual regulator SoxR
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (49/46); total (49/46)

TCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACA  >  NC_000913/4277754‑4278025

tCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGaaaaga                                                                                                                                      <  1:1133933/140‑1 (MQ=255)

      tGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTAc                                                                                                                                <  1:2135400/140‑1 (MQ=255)

       ggCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTAcc                                                                                                                               >  2:1202525/1‑140 (MQ=255)

         cGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCgg                                                                                                                             >  1:686420/1‑140 (MQ=255)

           agaagaGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGgcg                                                                                                                           >  1:2131792/1‑140 (MQ=255)

               gagTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACg                                                                                                                       <  2:1615606/140‑1 (MQ=255)

                 gTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCt                                                                                                                     >  1:1701518/1‑140 (MQ=255)

                 gTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCt                                                                                                                     <  1:2185938/140‑1 (MQ=255)

                  ttGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCtt                                                                                                                    >  2:318494/1‑140 (MQ=255)

                  ttGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCtt                                                                                                                    >  1:1704187/1‑140 (MQ=255)

                  ttGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCtt                                                                                                                    >  1:1327650/1‑140 (MQ=255)

                  ttGGATCGGCG‑ATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTg                                                                                                                   >  1:2045645/1‑140 (MQ=255)

                    ggATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGc                                                                                                                  >  1:1060941/1‑140 (MQ=255)

                      aTCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTg                                                                                                                <  1:909695/140‑1 (MQ=255)

                       tCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTgg                                                                                                               <  1:1188947/140‑1 (MQ=255)

                       tCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTgg                                                                                                               <  1:1024137/140‑1 (MQ=255)

                        cGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGa                                                                                                              <  2:1884686/140‑1 (MQ=255)

                          gcgcATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAg                                                                                                            >  1:986669/1‑140 (MQ=255)

                               ttCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGaa                                                                                                       <  1:318494/140‑1 (MQ=255)

                               ttCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGaa                                                                                                       <  2:1060941/140‑1 (MQ=255)

                               ttCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGaa                                                                                                       <  1:1198198/140‑1 (MQ=255)

                                        tAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaa                                                                                              >  2:1510118/1‑140 (MQ=255)

                                        tAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaa                                                                                              >  1:223341/1‑140 (MQ=255)

                                        tAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaa                                                                                              >  2:1578407/1‑140 (MQ=255)

                                        tAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaa                                                                                              >  1:1830269/1‑140 (MQ=255)

                                        tAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaa                                                                                              <  2:237844/140‑1 (MQ=255)

                                         aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaaa                                                                                             >  2:1538736/1‑140 (MQ=255)

                                         aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaaa                                                                                             >  1:527260/1‑140 (MQ=255)

                                         aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaaa                                                                                             >  2:404626/1‑140 (MQ=255)

                                         aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaaa                                                                                             >  1:1037559/1‑140 (MQ=255)

                                          gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                                            <  2:1327650/140‑1 (MQ=255)

                                          gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                                            >  2:1372299/1‑140 (MQ=255)

                                          gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                                            >  2:1807254/1‑140 (MQ=255)

                                          gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                                            >  1:2144071/1‑140 (MQ=255)

                                          gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                                            <  1:358165/140‑1 (MQ=255)

                                          gTGGCGCTGCGTGACGAACTGGACGGACGTATTGGTTGTGGCTGCTTTTCGCGCAGTGATTGCCCGTTGCGCAACACGGGCGGCCGCTTAGGAAAAGAACGTACCGGCGCACGCTTGCTGGAAGATGACCAAAACTaacg                                                                                            >  2:1068225/1‑138 (MQ=255)

                                           tGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCAC‑‑‑‑GCTGGAAGATGAACAAAACTAAAGCGCca                                                                                       >  1:682955/1‑140 (MQ=255)

                                              cgcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGcc                                                                                        >  2:1642440/1‑140 (MQ=255)

                                                cTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCcac                                                                                      >  1:300537/1‑140 (MQ=255)

                                                cTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCcac                                                                                      >  2:1583001/1‑140 (MQ=255)

                                                   cGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAg                                                                                   >  2:1563041/1‑140 (MQ=255)

                                                   cGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAg                                                                                   >  1:773109/1‑140 (MQ=255)

                                                   cGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCCCTTAGGAAAATAAGGTACCGGCGCACGCTTGCTTGAAGATGAACAAAACTAAAGCGCCACAAg                                                                                   >  1:2088985/1‑140 (MQ=255)

                                                     tGACGAACTGGACGGATGTATTGGTTGTAGCTGCCTTTAGAGCAGTCATTGCCCGTTGCGTAACACGGGCGACAGCCTAGGACAAGAAGATACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCTCAAggg                                                                                 <  2:2088985/140‑1 (MQ=255)

                                                        cGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGAGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGgcgc                                                                              >  2:1560672/1‑140 (MQ=255)

                                                         gAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCt                                                                             <  1:1510118/140‑1 (MQ=255)

                                                            cTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTa                                                                          <  2:300537/140‑1 (MQ=255)

                                                               gACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGTAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtt                                                                       >  1:770655/1‑140 (MQ=255)

                                                                    aTGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtttgttt                                                                  >  1:1049850/1‑140 (MQ=255)

                                                                            gTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtct                                                          <  1:1281635/140‑1 (MQ=255)

                                                                             ttGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctt                                                         <  1:839783/140‑1 (MQ=255)

                                                                              tgtgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtcttt                                                        >  1:817339/1‑140 (MQ=255)

                                                                              tgtgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtcttt                                                        >  1:380229/1‑140 (MQ=255)

                                                                                tgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgt                                                      >  1:1555517/1‑140 (MQ=255)

                                                                                 ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtc                                                     >  2:407251/1‑140 (MQ=255)

                                                                                 ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtc                                                     <  1:404626/140‑1 (MQ=255)

                                                                                 ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtc                                                     >  1:321150/1‑140 (MQ=255)

                                                                                 ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtc                                                     <  1:1578407/140‑1 (MQ=255)

                                                                                  gCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtct                                                    >  2:14439/1‑140 (MQ=255)

                                                                                   cTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtctt                                                   <  1:1085790/140‑1 (MQ=255)

                                                                                   cTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtctt                                                   <  1:1951671/140‑1 (MQ=255)

                                                                                    tGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtcttt                                                  <  2:321150/140‑1 (MQ=255)

                                                                                      ccTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTtct                                                >  2:2009416/1‑140 (MQ=255)

                                                                                      ccTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTtct                                                >  2:1778236/1‑140 (MQ=255)

                                                                                           cgcgCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTa                                           <  1:811613/140‑1 (MQ=255)

                                                                                            gcgcAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTAt                                          <  1:1538736/140‑1 (MQ=255)

                                                                                             cgcAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATc                                         <  2:1704187/140‑1 (MQ=255)

                                                                                              gcAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATcc                                        <  2:1701518/140‑1 (MQ=255)

                                                                                              gcAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATcc                                        <  1:2118298/140‑1 (MQ=255)

                                                                                              gcAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATcc                                        <  2:1550133/140‑1 (MQ=255)

                                                                                              gcAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATcc                                        <  1:763935/140‑1 (MQ=255)

                                                                                              gcAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATcc                                        <  1:1085355/140‑1 (MQ=255)

                                                                                              gcAGTGATTGCCAGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATcc                                        <  2:686420/140‑1 (MQ=255)

                                                                                                 gTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGc                                     <  2:412412/140‑1 (MQ=255)

                                                                                                 gTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGc                                     <  2:527260/140‑1 (MQ=255)

                                                                                                     ttGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGt                                 <  1:14439/140‑1 (MQ=255)

                                                                                                      tGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTa                                <  1:484926/140‑1 (MQ=255)

                                                                                                        cccGTTGCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTaca                              <  2:2131792/140‑1 (MQ=255)

                                                                                                          cGTTGCGTAACCCTGGCGACCGCTTAGGAAAATGAGGGACCAGGGCCCGCTTGCTGGAAGATGAACAAAACTAAAGCGCAACGAGGTCGCTTTAGTTTGTTTTCCGGTCATTGTCTTTCTCTCTATCCCGCTGGTAcaca                            <  2:424309/140‑1 (MQ=255)

                                                                                                              gCGTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACaggag                        >  1:867564/1‑140 (MQ=255)

                                                                                                                gTAACCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAggg                      >  2:370100/1‑140 (MQ=255)

                                                                                                                   aCCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGttt                   >  2:1217129/1‑140 (MQ=255)

                                                                                                                   aCCCGGGCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGttt                   <  1:1202525/140‑1 (MQ=255)

                                                                                                                        ggCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTccccc              <  2:2148517/140‑1 (MQ=255)

                                                                                                                        ggCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTccccc              <  1:1217129/140‑1 (MQ=255)

                                                                                                                         gCGACCGCTTAGGAAAAGAAGTTACCGGCGCACGCTTGCTGGAAGATTAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCg             >  2:1653617/1‑140 (MQ=255)

                                                                                                                         gCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTccccct             >  2:1128266/1‑139 (MQ=255)

                                                                                                                         gCGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCg             >  2:1421751/1‑140 (MQ=255)

                                                                                                                          cGACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGa            >  2:1212535/1‑140 (MQ=255)

                                                                                                                           gACCGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGAc           <  1:370100/140‑1 (MQ=255)

                                                                                                                              cGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTc        >  2:97052/1‑140 (MQ=255)

                                                                                                                              cGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTc        >  1:413064/1‑140 (MQ=255)

                                                                                                                              cGCTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTc        >  1:914588/1‑140 (MQ=255)

                                                                                                                                cTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCaa      <  1:2017837/140‑1 (MQ=255)

                                                                                                                                cTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCaa      <  2:1308050/140‑1 (MQ=255)

                                                                                                                                cTTAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCaa      <  1:880947/140‑1 (MQ=255)

                                                                                                                                  tAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAaca    <  1:2009416/140‑1 (MQ=255)

                                                                                                                                  tAGGAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAaca    <  1:2082222/140‑1 (MQ=255)

                                                                                                                                    ggAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAacaca  <  2:315764/140‑1 (MQ=255)

                                                                                                                                    ggAAAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAacaca  <  1:1807254/140‑1 (MQ=255)

TCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACA  >  NC_000913/4277754‑4278025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A