Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F48 I1 R1 55 131.7 4456318 99.2% 4420667 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,261,710 C→A S14R (AGC→AGA)  dusA → tRNA‑dihydrouridine synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,261,7100CA99.4% 599.4 / NA 169S14R (AGC→AGA) dusAtRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (92/76);  total (93/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

TTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261574‑4261844
                                                                                                                                        |                                                                                                                                       
ttATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCa                                                                                                                                      >  1:1343189/1‑140 (MQ=255)
           tACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaa                                                                                                                           >  2:1203046/1‑140 (MQ=255)
             ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                         >  1:2183011/1‑140 (MQ=255)
             ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                         >  2:466114/1‑140 (MQ=255)
             ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                         >  1:1311895/1‑140 (MQ=255)
               ccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACt                                                                                                                       >  2:253499/1‑140 (MQ=255)
               ccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACt                                                                                                                       >  1:1796576/1‑140 (MQ=255)
                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  2:540362/1‑140 (MQ=255)
                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  1:1899435/1‑140 (MQ=255)
                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  1:1777240/1‑140 (MQ=255)
                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  2:672145/1‑140 (MQ=255)
                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  1:557688/1‑140 (MQ=255)
                  cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                    >  1:937288/1‑140 (MQ=255)
                  cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                    <  2:1516149/140‑1 (MQ=255)
                   tATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACg                                                                                                                   <  2:1331282/140‑1 (MQ=255)
                   tATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACg                                                                                                                   <  2:1234120/140‑1 (MQ=255)
                     ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                    <  1:2032569/137‑1 (MQ=255)
                     ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGtt                                                                                                                 >  1:1537828/1‑140 (MQ=255)
                       gAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCa                                                                                                               <  1:513986/140‑1 (MQ=255)
                        aGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCAc                                                                                                              >  1:95344/1‑140 (MQ=255)
                            tcctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGa                                                                                                          >  2:2001867/1‑140 (MQ=255)
                               tGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTg                                                                                                      >  1:1812520/1‑140 (MQ=255)
                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGt                                                                                                    <  2:1835083/140‑1 (MQ=255)
                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGt                                                                                                    <  2:586378/140‑1 (MQ=255)
                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGt                                                                                                    <  1:433941/140‑1 (MQ=255)
                                      tGATGCTGAATAACGTAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGtt                                                                                               >  1:757870/1‑140 (MQ=255)
                                      tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGtt                                                                                               >  1:1729158/1‑140 (MQ=255)
                                      tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGtt                                                                                               >  2:63758/1‑140 (MQ=255)
                                      tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGtt                                                                                               >  1:889841/1‑140 (MQ=255)
                                       gATGCTGAATAACGTAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGttt                                                                                              <  2:757870/140‑1 (MQ=255)
                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGt                                                                                                    <  1:807598/133‑1 (MQ=255)
                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGt                                                                                                    >  2:807598/1‑133 (MQ=255)
                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTa                                                                                             >  2:1194076/1‑140 (MQ=255)
                                         tGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAg                                                                                            <  1:1302929/140‑1 (MQ=255)
                                           cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGct                                                                                          >  2:41683/1‑139 (MQ=255)
                                             gAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGtt                                                                                        <  1:964380/140‑1 (MQ=255)
                                              aaTAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTg                                                                                       >  1:548341/1‑140 (MQ=255)
                                                   cataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAAAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCa                                                                                  >  2:770799/1‑140 (MQ=255)
                                                      aacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATg                                                                               <  2:95344/140‑1 (MQ=255)
                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  1:2100063/1‑140 (MQ=255)
                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:217818/1‑140 (MQ=255)
                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  1:1014986/1‑140 (MQ=255)
                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGC‑AAAAATCCACCAAAACAAAAGAATGCCCTAAAAAACTGACGGTCAACGGAGGGGGCCGGTTAACCGTTCCACAAATCCt                                                                             >  2:2035890/1‑140 (MQ=255)
                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGtt                                                                                        <  1:540190/124‑1 (MQ=255)
                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGtt                                                                                        >  2:540190/1‑124 (MQ=255)
                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:1457774/1‑140 (MQ=255)
                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:834331/1‑140 (MQ=255)
                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  2:1409990/1‑140 (MQ=255)
                                                              gATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTg                                                                       >  1:2227382/1‑140 (MQ=255)
                                                               atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:1796576/140‑1 (MQ=255)
                                                                tatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     <  2:1014986/140‑1 (MQ=255)
                                                                  tatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacg                                                                   >  2:1922787/1‑140 (MQ=255)
                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:1329431/1‑140 (MQ=255)
                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:382491/1‑140 (MQ=255)
                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:143506/1‑140 (MQ=255)
                                                                    tCGATAAAATAGTCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCACAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgga                                                                 >  2:2022460/1‑140 (MQ=255)
                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacggac                                                                <  1:1477459/138‑1 (MQ=255)
                                                                         aaaaTAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacagac                                                            >  2:1949683/1‑140 (MQ=255)
                                                                           aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                          <  1:1186295/140‑1 (MQ=255)
                                                                              aatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGc                                                       <  2:1003976/140‑1 (MQ=255)
                                                                               atCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGcc                                                      <  2:1899435/140‑1 (MQ=255)
                                                                                  tctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCt                                                   >  2:746347/1‑140 (MQ=255)
                                                                                   ctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTa                                                  <  1:1997993/140‑1 (MQ=255)
                                                                                    tACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAt                                                 >  2:374178/1‑140 (MQ=255)
                                                                                     aCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAtt                                                >  1:264075/1‑140 (MQ=255)
                                                                                     aCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAtt                                                >  2:268294/1‑140 (MQ=255)
                                                                                         ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCtt                                            >  1:1132472/1‑140 (MQ=255)
                                                                                              aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTATCGTTGCACCAATGCTCGACTTGACGGCCAGACATTGCCGCTATTTCTTGCGtt                                       >  2:1890538/1‑139 (MQ=255)
                                                                                                tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCtt                                     >  2:818068/1‑139 (MQ=255)
                                                                                                tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     >  1:828454/1‑140 (MQ=255)
                                                                                                tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     >  2:1896771/1‑140 (MQ=255)
                                                                                                tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     >  1:273843/1‑140 (MQ=255)
                                                                                                   aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                  <  2:1329431/140‑1 (MQ=255)
                                                                                                      gTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTcc                               <  2:1729158/140‑1 (MQ=255)
                                                                                                      gTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTcc                               <  1:466114/140‑1 (MQ=255)
                                                                                                        aaTTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCg                             <  2:1577631/140‑1 (MQ=255)
                                                                                                        aaTTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCg                             <  1:63758/140‑1 (MQ=255)
                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGTCGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  1:672145/140‑1 (MQ=255)
                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  1:1011619/140‑1 (MQ=255)
                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  2:2227382/140‑1 (MQ=255)
                                                                                                            cTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAAt                         <  2:1132472/140‑1 (MQ=255)
                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:889841/140‑1 (MQ=255)
                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:834331/140‑1 (MQ=255)
                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATAct                      >  2:5827/1‑139 (MQ=255)
                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:1457774/140‑1 (MQ=255)
                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:2100063/140‑1 (MQ=255)
                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:378438/140‑1 (MQ=255)
                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:1090882/140‑1 (MQ=255)
                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:5827/140‑1 (MQ=255)
                                                                                                                aaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGt                     <  2:1807237/140‑1 (MQ=255)
                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  2:2181305/1‑140 (MQ=255)
                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  1:795297/1‑140 (MQ=255)
                                                                                                                  tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:1174129/140‑1 (MQ=255)
                                                                                                                  tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:552690/140‑1 (MQ=255)
                                                                                                                   gCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGc                  <  1:1896771/140‑1 (MQ=255)
                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 >  2:299660/1‑140 (MQ=255)
                                                                                                                             aaccaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGa        >  2:442986/1‑140 (MQ=255)
                                                                                                                              accaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGaa       <  1:1194076/140‑1 (MQ=255)
                                                                                                                                 aaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATg    <  2:1124573/140‑1 (MQ=255)
                                                                                                                                   accaGAGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  2:1537828/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGA‑GTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261574‑4261844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A