Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F48 I1 R1	61	74.3	2613450	99.1%	2589928	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,294,762	C→A	V94V (GTC→GTA)	gltP →	glutamate/aspartate : H(+) symporter GltP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,294,762	0	C	A	100.0%	191.0 / NA	54	V94V (GTC→GTA)	gltP	glutamate/aspartate : H(+) symporter GltP
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/28); total (26/28)

TCTGATTAAAATGATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTCGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCAGTTCCCACGGCATT  >  NC_000913/4294630‑4294897

tcTGATTAAAATGATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCcatca                                                                                                                                  >  1:1278715/1‑140 (MQ=255)

 cTGATTAAAATGATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCcatcat                                                                                                                                 >  2:622325/1‑140 (MQ=255)

  tGATTAAAATGATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCAtt                                                                                                                                <  1:31540/140‑1 (MQ=255)

   gATTAAAATGATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCAttt                                                                                                                               <  2:911884/140‑1 (MQ=255)

    aTTAAAATGATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCA‑CATTTTg                                                                                                                             >  1:428553/1‑140 (MQ=255)

            gATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCa                                                                                                                      <  1:106190/140‑1 (MQ=255)

              ttgttgTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCAct                                                                                                                    >  2:295750/1‑140 (MQ=255)

              ttgttgTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCAct                                                                                                                    >  1:1127623/1‑140 (MQ=255)

              ttgttgTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCAct                                                                                                                    >  1:983647/1‑140 (MQ=255)

               tgttgtGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCActc                                                                                                                   >  1:72087/1‑140 (MQ=255)

                  tgtgCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTgg                                                                                                                >  2:311478/1‑140 (MQ=255)

                   gtgCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGc                                                                                                               >  2:979980/1‑140 (MQ=255)

                      ccGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGaa                                                                                                            <  1:329435/140‑1 (MQ=255)

                      ccGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGaa                                                                                                            >  1:72564/1‑140 (MQ=255)

                         aTTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGt                                                                                                         >  1:179804/1‑140 (MQ=255)

                             tgATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTc                                                                                                     <  1:979980/140‑1 (MQ=255)

                                  tcCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGcc                                                                                                <  2:537624/140‑1 (MQ=255)

                                  tcCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGcc                                                                                                <  1:198418/140‑1 (MQ=255)

                                    cACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCg                                                                                              >  2:563251/1‑140 (MQ=255)

                                    cACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCg                                                                                              >  2:619343/1‑140 (MQ=255)

                                       gCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTg                                                                                           <  2:179804/140‑1 (MQ=255)

                                        cTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGc                                                                                          <  1:669739/140‑1 (MQ=255)

                                        cTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGc                                                                                          <  1:295750/140‑1 (MQ=255)

                                          ggtggtggGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCg                                                                                        <  2:952050/140‑1 (MQ=255)

                                           gtggtggGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCgg                                                                                       >  1:427775/1‑140 (MQ=255)

                                           gtggtggGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCgg                                                                                       <  1:404464/140‑1 (MQ=255)

                                           gtggtggGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCgg                                                                                       >  2:458245/1‑140 (MQ=255)

                                           gtggtggGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCgg                                                                                       >  2:808972/1‑140 (MQ=255)

                                           gtggtggGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCgg                                                                                       >  2:977593/1‑140 (MQ=255)

                                            tggtggGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCggg                                                                                      <  2:412276/140‑1 (MQ=255)

                                            tggtggGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCggg                                                                                      <  1:622325/140‑1 (MQ=255)

                                                                     tGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCg                                                             >  1:263859/1‑140 (MQ=255)

                                                                           aCAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGata                                                       <  2:72564/140‑1 (MQ=255)

                                                                              gCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATAtct                                                    <  2:72087/140‑1 (MQ=255)

                                                                               cTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATAtctc                                                   >  1:999591/1‑140 (MQ=255)

                                                                                tCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCg                                                  <  2:263859/140‑1 (MQ=255)

                                                                                         ttGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCag                                         >  2:1207666/1‑140 (MQ=255)

                                                                                           ggCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCagag                                       <  1:311478/140‑1 (MQ=255)

                                                                                            gcgcGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGc                                      <  2:999591/140‑1 (MQ=255)

                                                                                            gcgcGAAAACCATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGc                                      <  1:977593/140‑1 (MQ=255)

                                                                                                      cATTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAg                            <  2:427775/140‑1 (MQ=255)

                                                                                                      aaTTATCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAg                            <  2:444980/139‑1 (MQ=255)

                                                                                                           tCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTa                       <  2:805626/140‑1 (MQ=255)

                                                                                                           tCTACTTCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTa                       <  2:983647/140‑1 (MQ=255)

                                                                                                                ttCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAg                  >  2:30722/1‑140 (MQ=255)

                                                                                                                ttCGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAg                  >  1:550880/1‑140 (MQ=255)

                                                                                                                ttCGAGGCGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAg                  >  1:102196/1‑140 (MQ=255)

                                                                                                                  cGAGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCa                >  1:984375/1‑140 (MQ=255)

                                                                                                                  cGAGGTGATCACCACCGTAGCCATCATCTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCa                <  1:808972/140‑1 (MQ=255)

                                                                                                                    aGGTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCAGt              <  2:550880/140‑1 (MQ=255)

                                                                                                                     ggTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCAGtt             >  1:998571/1‑140 (MQ=255)

                                                                                                                     ggTGATCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCAGtt             >  2:909890/1‑140 (MQ=255)

                                                                                                                         aTCACCACCGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCAGTTCCCa         <  2:1268383/140‑1 (MQ=255)

                                                                                                                                ccGTAGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCAGTTCCCACGGCAtt  <  2:1278715/140‑1 (MQ=255)

TCTGATTAAAATGATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATTGGCGCGAAAACCATTATCTACTTCGAGGTGATCACCACCGTCGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCAGCCCGGTGCCGGGGTGGATATGTCGCAGTTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAAGCAGTTCCCACGGCATT  >  NC_000913/4294630‑4294897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A