Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F48 I1 R1	61	74.3	2613450	99.1%	2589928	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,445,749	G→T	A546A (GCG→GCT)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,445,749	0	G	T	100.0%	166.9 / NA	49	A546A (GCG→GCT)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (24/25); total (24/25)

TTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCG  >  NC_000913/4445613‑4445882

ttGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCg                                                                                                                                    >  2:60268/1‑140 (MQ=255)

ttGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCg                                                                                                                                    <  2:331470/140‑1 (MQ=255)

   aCGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCgggg                                                                                                                                 >  2:20102/1‑140 (MQ=255)

   aCGGCACGATGAAAGGCAGCAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCAACCATCAACGCTCCgggg                                                                                                                                 <  1:305944/140‑1 (MQ=255)

    cGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGc                                                                                                                                >  1:522793/1‑140 (MQ=255)

             gAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAAcg                                                                                                                       <  2:899420/140‑1 (MQ=255)

                   cAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGc                                                                                                                 <  2:517860/140‑1 (MQ=255)

                             atGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTgg                                                                                                       <  1:966059/140‑1 (MQ=255)

                                   tGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCt                                                                                                          <  2:299453/131‑1 (MQ=255)

                                   tGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCt                                                                                                          >  1:299453/1‑131 (MQ=255)

                                   tGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTAc                                                                                                 >  1:1077010/1‑140 (MQ=255)

                                   tGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTAc                                                                                                 >  1:545462/1‑140 (MQ=255)

                                             cAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAggg                                                                                       <  1:1027829/140‑1 (MQ=255)

                                             cAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAggg                                                                                       <  1:889127/140‑1 (MQ=255)

                                             cAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAggg                                                                                       <  1:508406/140‑1 (MQ=255)

                                               gggCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCt                                                                                     >  1:48804/1‑140 (MQ=255)

                                                ggCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTg                                                                                    >  2:1259399/1‑140 (MQ=255)

                                                    tCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGa                                                                                >  2:945103/1‑140 (MQ=255)

                                                     cATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGaa                                                                               >  2:283427/1‑140 (MQ=255)

                                                     cATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGaa                                                                               >  2:1199860/1‑140 (MQ=255)

                                                     cATCTGGAACTCGGGCCAAACAGTACCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTAATGCCAGCATCAACGCTCCGGTGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGaa                                                                               >  2:1227870/1‑140 (MQ=255)

                                                         tGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTa                                                                           <  2:522793/140‑1 (MQ=255)

                                                         tGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTa                                                                           <  1:945103/140‑1 (MQ=255)

                                                           gAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTAcg                                                                         <  2:784213/140‑1 (MQ=255)

                                                           gAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTAcg                                                                         <  2:1238059/140‑1 (MQ=255)

                                                                 gggCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCAc                                                                   >  1:926489/1‑140 (MQ=255)

                                                                        aCAGTGGCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCAACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGaa                                                            <  2:372543/140‑1 (MQ=255)

                                                                        aCAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGaa                                                            <  1:1259399/140‑1 (MQ=255)

                                                                        aCAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGaa                                                            <  2:1156550/140‑1 (MQ=255)

                                                                             gCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGcc                                                       >  2:392412/1‑140 (MQ=255)

                                                                             gCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGcc                                                       >  1:564217/1‑140 (MQ=255)

                                                                               cGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCcac                                                     <  1:283427/140‑1 (MQ=255)

                                                                                gAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCcaca                                                    >  1:1001487/1‑140 (MQ=255)

                                                                                    tGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAacta                                                <  1:968540/140‑1 (MQ=255)

                                                                                     gAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAactac                                               <  2:1077010/140‑1 (MQ=255)

                                                                                       aaGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTg                                             <  2:564217/140‑1 (MQ=255)

                                                                                         ggCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGc                                           <  1:60268/140‑1 (MQ=255)

                                                                                         agCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGc                                           <  2:444035/139‑1 (MQ=255)

                                                                                           cGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAg                                         >  1:408171/1‑140 (MQ=255)

                                                                                             aGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGat                                       >  2:201567/1‑140 (MQ=255)

                                                                                              gCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGata                                      >  1:595299/1‑140 (MQ=255)

                                                                                                  ggggTAAAAGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCAc                                  <  2:595299/140‑1 (MQ=255)

                                                                                                        aaaGATCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACcgcgcg                            <  2:48804/140‑1 (MQ=255)

                                                                                                            aTCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGc                        <  1:392412/140‑1 (MQ=255)

                                                                                                            aTCTCAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGc                        >  1:1141617/1‑140 (MQ=255)

                                                                                                             tctcAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGcc                       >  1:365354/1‑140 (MQ=255)

                                                                                                              ctcAATCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCt                      >  1:402245/1‑140 (MQ=255)

                                                                                                                 aaTCTTGATGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTgcg                   <  1:1199860/140‑1 (MQ=255)

                                                                                                                        aTGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAg            >  1:1247830/1‑140 (MQ=255)

                                                                                                                        aTGCCACCATCAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAg            >  2:394179/1‑140 (MQ=255)

                                                                                                                                  cAACGCTCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGAGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCg  <  1:1227870/140‑1 (MQ=255)

TTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCG  >  NC_000913/4445613‑4445882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A