Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F48 I1 R1 56 123.4 4244010 99.1% 4205813 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,180,365 G→T D124Y (GAC→TAC)  rplJ → 50S ribosomal subunit protein L10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,180,3650GT99.2% 444.3 / ‑3.0 126D124Y (GAC→TAC) rplJ50S ribosomal subunit protein L10
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (73/52);  total (73/53)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

Only 100 of 127 total aligned reads displayed.

CCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCA  >  NC_000913/4180235‑4180498
                                                                                                                                  |                                                                                                                                     
ccTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTgg                                                                                                                              <  2:379951/140‑1 (MQ=255)
   gATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCaa                                                                                                                           <  1:449719/140‑1 (MQ=255)
   gATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCaa                                                                                                                           <  1:1802127/140‑1 (MQ=255)
    aTTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAAc                                                                                                                          >  1:533529/1‑140 (MQ=255)
    aTTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAAc                                                                                                                          >  1:1355933/1‑140 (MQ=255)
      tGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAActc                                                                                                                        >  2:1068335/1‑140 (MQ=255)
        cATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTg                                                                                                                      <  1:1983295/140‑1 (MQ=255)
         aTACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGc                                                                                                                     >  2:1533406/1‑140 (MQ=255)
         aTACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGc                                                                                                                     >  2:53996/1‑140 (MQ=255)
         aTACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGc                                                                                                                     >  2:1234310/1‑140 (MQ=255)
             tctATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGAc                                                                                                                 >  2:635068/1‑140 (MQ=255)
              ctATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGAcc                                                                                                                >  1:1119065/1‑140 (MQ=255)
              ctATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGAcc                                                                                                                >  1:76868/1‑140 (MQ=255)
              ctATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGAcc                                                                                                                >  2:420178/1‑140 (MQ=255)
              ctATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGAcc                                                                                                                >  1:1743245/1‑140 (MQ=255)
                 tGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTAc                                                                                                             >  2:928291/1‑140 (MQ=255)
                    aaCACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaa                                                                                                          >  2:1009596/1‑140 (MQ=255)
                    aaCACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaa                                                                                                          >  2:768982/1‑140 (MQ=255)
                      cacCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaaga                                                                                                        >  1:1493866/1‑140 (MQ=255)
                      cacCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaaga                                                                                                        >  2:1660990/1‑140 (MQ=255)
                       acCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaagaa                                                                                                       >  2:1886116/1‑140 (MQ=255)
                       acCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaagaa                                                                                                       >  2:684149/1‑140 (MQ=255)
                       acCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaagaa                                                                                                       >  2:1900598/1‑140 (MQ=255)
                       acCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaagaa                                                                                                       >  2:355594/1‑140 (MQ=255)
                       acCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaagaa                                                                                                       >  2:1205717/1‑140 (MQ=255)
                       acCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaagaa                                                                                                       >  2:871078/1‑140 (MQ=255)
                       acCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaagaa                                                                                                       >  2:1334423/1‑140 (MQ=255)
                       acCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACgaagaa                                                                                                       >  2:908869/1‑140 (MQ=255)
                         ccGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGTGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGc                                                                                                     >  2:2005595/1‑140 (MQ=255)
                         ccGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGc                                                                                                     >  1:1575102/1‑140 (MQ=255)
                         ccGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGc                                                                                                     >  2:528507/1‑140 (MQ=255)
                         ccGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGc                                                                                                     <  1:2104959/140‑1 (MQ=255)
                         ccGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGc                                                                                                     <  1:482476/140‑1 (MQ=255)
                           gggCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCaa                                                                                                   >  2:457477/1‑140 (MQ=255)
                           gggCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCaa                                                                                                   >  2:1587372/1‑140 (MQ=255)
                              cgcTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAAttt                                                                                                >  2:421360/1‑139 (MQ=255)
                                ctgctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCa                                                                                              <  1:49416/140‑1 (MQ=255)
                                ctgctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCa                                                                                              <  2:1493866/140‑1 (MQ=255)
                                ctgctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCa                                                                                              <  1:2033817/140‑1 (MQ=255)
                                ctgctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCa                                                                                              <  1:1596016/140‑1 (MQ=255)
                                   ctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGc                                                                                           <  2:564104/140‑1 (MQ=255)
                                   ctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGc                                                                                           <  1:1587372/140‑1 (MQ=255)
                                   ctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGc                                                                                           >  2:320672/1‑140 (MQ=255)
                                   ctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGc                                                                                           >  2:715416/1‑140 (MQ=255)
                                    tgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGTGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGcc                                                                                          <  1:2005595/140‑1 (MQ=255)
                                          tCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATgg                                                                                    <  1:51519/140‑1 (MQ=255)
                                           cTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGATCTACGAAGAAGCAATTGCACGCCTGATGGc                                                                                   <  2:988147/140‑1 (MQ=255)
                                           cTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGc                                                                                   >  1:1125565/1‑140 (MQ=255)
                                           cTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGc                                                                                   <  1:1234310/140‑1 (MQ=255)
                                           cTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGc                                                                                   <  1:871078/140‑1 (MQ=255)
                                           cTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGCGAGCTGATCCCGGCGTCTCAGATCTACCTCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGc                                                                                   >  1:629689/1‑140 (MQ=255)
                                             gTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCaa                                                                                 >  2:1830130/1‑140 (MQ=255)
                                                     agTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGaaa                                                                         <  1:1009596/140‑1 (MQ=255)
                                                     agTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGaaa                                                                         <  1:908869/140‑1 (MQ=255)
                                                      gTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaag                                                                        <  1:528507/140‑1 (MQ=255)
                                                        tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaagaa                                                                      >  2:104505/1‑140 (MQ=255)
                                                        tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaagaa                                                                      >  1:2070213/1‑140 (MQ=255)
                                                        tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaagaa                                                                      >  1:762320/1‑140 (MQ=255)
                                                        tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaagaa                                                                      >  2:133646/1‑140 (MQ=255)
                                                        tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaagaa                                                                      >  1:1496799/1‑140 (MQ=255)
                                                        tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaagaa                                                                      >  1:1502246/1‑140 (MQ=255)
                                                        tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaagaa                                                                      >  2:71944/1‑140 (MQ=255)
                                                        tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAaagaa                                                                      >  2:842631/1‑140 (MQ=255)
                                                                     tGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCa                                                         <  1:69382/140‑1 (MQ=255)
                                                                       cAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCaaa                                                       <  1:1984236/140‑1 (MQ=255)
                                                                       cAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCaaa                                                       <  1:1368273/140‑1 (MQ=255)
                                                                       cAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCaaa                                                       <  1:133646/140‑1 (MQ=255)
                                                                       aaaaaTTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCaaa                                                       <  2:15445/139‑1 (MQ=255)
                                                                         aaaTTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACt                                                     <  2:2070213/140‑1 (MQ=255)
                                                                            tttGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGt                                                  <  1:1220393/140‑1 (MQ=255)
                                                                            tttGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGt                                                  <  2:181102/140‑1 (MQ=255)
                                                                                  gTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTAc                                            >  1:1826016/1‑140 (MQ=255)
                                                                                   tCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTAct                                           <  2:1575102/140‑1 (MQ=255)
                                                                                      aaGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTg                                        <  2:990284/140‑1 (MQ=255)
                                                                                       aGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTgg                                       <  2:1638732/140‑1 (MQ=255)
                                                                                       aGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTgg                                       >  1:1586656/1‑140 (MQ=255)
                                                                                       aGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTgg                                       >  2:79827/1‑140 (MQ=255)
                                                                                       aGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTgg                                       <  1:245831/140‑1 (MQ=255)
                                                                                          cGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGgctg                                    <  2:1125565/140‑1 (MQ=255)
                                                                                          cGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGgctg                                    <  1:1900598/140‑1 (MQ=255)
                                                                                            cTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGgctgct                                  >  2:1300/1‑140 (MQ=255)
                                                                                               ccTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTa                               <  1:635068/140‑1 (MQ=255)
                                                                                                 tttGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTAcg                             <  1:104505/140‑1 (MQ=255)
                                                                                                  ttGAAGGTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCATCCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTAcgc                            <  1:1660990/140‑1 (MQ=255)
                                                                                                        gTGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCg                      <  1:1254916/140‑1 (MQ=255)
                                                                                                         tGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGa                     >  2:594745/1‑140 (MQ=255)
                                                                                                         tGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGa                     >  2:1189861/1‑140 (MQ=255)
                                                                                                         tGAGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGa                     >  1:1681861/1‑140 (MQ=255)
                                                                                                           aGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGaaa                   >  2:907217/1‑140 (MQ=255)
                                                                                                           aGCTGATCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGaaa                   >  1:1948781/1‑140 (MQ=255)
                                                                                                                 tCCCGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAgct             >  1:211855/1‑140 (MQ=255)
                                                                                                                  cccGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAgctg            >  1:654109/1‑140 (MQ=255)
                                                                                                                   ccGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAgctgc           <  1:591517/140‑1 (MQ=255)
                                                                                                                   ccGGCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAgctgc           <  1:928291/140‑1 (MQ=255)
                                                                                                                     ggCGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCtt         >  1:989298/1‑140 (MQ=255)
                                                                                                                       cGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTATGCGATGCGAAAGAAGCTGCTTaa       <  1:420178/140‑1 (MQ=255)
                                                                                                                       cGTCTCAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTaa       <  1:1300/140‑1 (MQ=255)
                                                                                                                          ctcAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGTTTAATCg    >  2:1516254/1‑140 (MQ=255)
                                                                                                                          ctcAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCg    >  1:1349826/1‑140 (MQ=255)
                                                                                                                            cAGATCTACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGGAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCa  <  1:715416/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
CCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCA  >  NC_000913/4180235‑4180498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A