Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F48 I1 R1	70	151.6	5072932	98.7%	5006983	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,431,335	C→A	T27T (ACG→ACT)	tsaC ←	threonylcarbamoyl‑AMP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,431,335	0	C	A	100.0%	476.5 / NA	133	T27T (ACG→ACT)	tsaC	threonylcarbamoyl‑AMP synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (69/64); total (69/64)

Only 100 of 135 total aligned reads displayed.

ATATAGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGT  >  NC_000913/3431201‑3431466

atatAGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTgg                                                                                                                                >  2:1740665/1‑140 (MQ=255)

 tataGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGa                                                                                                                               <  2:1602208/140‑1 (MQ=255)

 tataGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGa                                                                                                                               <  1:2090543/140‑1 (MQ=255)

 tataGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGa                                                                                                                               <  2:2101614/140‑1 (MQ=255)

  ataGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGAt                                                                                                                              >  1:2525630/1‑140 (MQ=255)

  ataGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGAt                                                                                                                              >  2:71447/1‑140 (MQ=255)

    aGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAg                                                                                                                            >  2:876748/1‑140 (MQ=255)

      ggTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGc                                                                                                                          >  1:1426022/1‑140 (MQ=255)

      ggTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGc                                                                                                                          >  1:1785301/1‑140 (MQ=255)

        tttAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGa                                                                                                                        >  1:1134882/1‑140 (MQ=255)

         ttAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGAt                                                                                                                       >  1:1098610/1‑140 (MQ=255)

           aaGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGa                                                                                                                     >  2:817684/1‑140 (MQ=255)

            aGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGac                                                                                                                    >  2:1962348/1‑140 (MQ=255)

            aGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGac                                                                                                                    <  2:1785301/140‑1 (MQ=255)

            aGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGac                                                                                                                    >  1:2440057/1‑140 (MQ=255)

              ctgctCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGacac                                                                                                                  <  2:1426022/140‑1 (MQ=255)

              ctgatCGTAATTTGCTGCGATTAAAACCAGCCCCTTATCAACCGGACGCTGTTGTAACTCAAACAGTCGCAACACTGCTGTTTCGCTCTCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGacac                                                                                                                  <  2:2267022/140‑1 (MQ=255)

                gctCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGt                                                                                                                >  1:2415396/1‑140 (MQ=255)

                 ctCGTAATTTGCTGCGATTAAAATCAGGCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGtt                                                                                                               >  1:2056408/1‑140 (MQ=255)

                 ctCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGtt                                                                                                               >  1:2285795/1‑140 (MQ=255)

                 ctCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGtt                                                                                                               >  2:2252119/1‑140 (MQ=255)

                 ctCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGtt                                                                                                               >  2:2322251/1‑140 (MQ=255)

                 ctCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGtt                                                                                                               >  2:236381/1‑140 (MQ=255)

                 ctCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGtt                                                                                                               >  1:728553/1‑140 (MQ=255)

                      aaTTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCa                                                                                                          <  2:1914331/140‑1 (MQ=255)

                        tttGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCAtt                                                                                                        <  2:1046995/140‑1 (MQ=255)

                         ttGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTg                                                                                                       <  2:1450583/140‑1 (MQ=255)

                                     aaaTCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATc                                                                                           <  1:2309864/140‑1 (MQ=255)

                                       aTCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGc                                                                                         <  2:1134882/140‑1 (MQ=255)

                                          aGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGc                                                                                      >  2:1528648/1‑140 (MQ=255)

                                           gCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCt                                                                                     >  1:1281864/1‑140 (MQ=255)

                                           gCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCt                                                                                     <  1:1740665/140‑1 (MQ=255)

                                           gCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCt                                                                                     >  2:2286584/1‑140 (MQ=255)

                                            ccccTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTg                                                                                    >  2:555066/1‑140 (MQ=255)

                                            ccccTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTg                                                                                    >  2:2205333/1‑140 (MQ=255)

                                            ccccTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTg                                                                                    >  2:172648/1‑140 (MQ=255)

                                            ccccTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTg                                                                                    >  2:460217/1‑140 (MQ=255)

                                                ttATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGAt                                                                                >  1:369809/1‑140 (MQ=255)

                                                 tATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATa                                                                               >  1:2154174/1‑140 (MQ=255)

                                                  aTCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGATACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATat                                                                              >  1:1356663/1‑139 (MQ=255)

                                                     aaCCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGt                                                                           <  1:2252119/140‑1 (MQ=255)

                                                     aaCCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGt                                                                           <  1:2006847/140‑1 (MQ=255)

                                                     aaCCGGACGCTGTTGTAGCTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAGCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGt                                                                           <  1:2205333/140‑1 (MQ=255)

                                                      aCCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGtc                                                                          >  1:822863/1‑140 (MQ=255)

                                                        cGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGtctc                                                                        >  1:2536109/1‑140 (MQ=255)

                                                          gACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCtt                                                                      >  2:420901/1‑140 (MQ=255)

                                                             gCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGc                                                                   <  2:2147446/140‑1 (MQ=255)

                                                             gCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGc                                                                   <  2:2440057/140‑1 (MQ=255)

                                                              cTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCa                                                                  <  1:876748/140‑1 (MQ=255)

                                                              cTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCa                                                                  <  1:16481/140‑1 (MQ=255)

                                                               tGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAg                                                                 >  2:2481/1‑140 (MQ=255)

                                                                 ttttAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGt                                                               <  1:1962348/140‑1 (MQ=255)

                                                                 ttttAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGt                                                               <  1:71447/140‑1 (MQ=255)

                                                                 ttttAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCGTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGt                                                               >  1:613211/1‑140 (MQ=255)

                                                                       cTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTtattat                                                         <  2:109151/140‑1 (MQ=255)

                                                                        tCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTattatt                                                        <  1:1334957/140‑1 (MQ=255)

                                                                        tCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTattatt                                                        <  2:1611588/140‑1 (MQ=255)

                                                                        tCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTattatt                                                        <  2:1338209/140‑1 (MQ=255)

                                                                          cAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCa                                                      <  2:2056408/140‑1 (MQ=255)

                                                                          aaaCAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCa                                                      <  2:1281864/139‑1 (MQ=255)

                                                                           aaCAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTattatt                                                        >  1:967133/1‑137 (MQ=255)

                                                                           aaCAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCAc                                                     >  2:869993/1‑140 (MQ=255)

                                                                           aaCAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCAc                                                     <  1:555066/140‑1 (MQ=255)

                                                                           aaCAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCAc                                                     >  2:300273/1‑140 (MQ=255)

                                                                                     tCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTc                                           >  1:2006657/1‑140 (MQ=255)

                                                                                     tCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTc                                           <  1:1513683/140‑1 (MQ=255)

                                                                                     tCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTc                                           >  1:2058504/1‑140 (MQ=255)

                                                                                        ctgctgTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTccgc                                        >  1:1136460/1‑140 (MQ=255)

                                                                                         tgctgTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTccgcc                                       >  1:2434591/1‑140 (MQ=255)

                                                                                          gctgTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTccgccg                                      >  1:1496625/1‑140 (MQ=255)

                                                                                           ctgTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGa                                     >  1:884982/1‑140 (MQ=255)

                                                                                              tttCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAAc                                  >  2:501631/1‑140 (MQ=255)

                                                                                              tttCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAAc                                  <  1:420901/140‑1 (MQ=255)

                                                                                                tCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCTGAAACCg                                <  1:1092355/140‑1 (MQ=255)

                                                                                                tCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAAcct                                >  2:376554/1‑139 (MQ=255)

                                                                                                   cTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCt                             >  1:2402968/1‑140 (MQ=255)

                                                                                                   cTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCt                             <  1:2174313/140‑1 (MQ=255)

                                                                                                   cTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCt                             >  1:995094/1‑140 (MQ=255)

                                                                                                   cTATCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCt                             >  1:733670/1‑140 (MQ=255)

                                                                                                     aTCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCttt                           <  1:501631/140‑1 (MQ=255)

                                                                                                     aTCAGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCttt                           <  2:1098610/140‑1 (MQ=255)

                                                                                                       aaGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTcc                         <  1:1003144/139‑1 (MQ=255)

                                                                                                        aGGATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCca                        <  2:1496625/140‑1 (MQ=255)

                                                                                                          gATCGCACCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCcaca                      <  1:144167/140‑1 (MQ=255)

                                                                                                                aCCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGttt                >  1:1685061/1‑140 (MQ=255)

                                                                                                                aCCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGttt                >  1:1117316/1‑140 (MQ=255)

                                                                                                                aCCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGttt                >  1:328024/1‑140 (MQ=255)

                                                                                                                aCCCAACACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGttt                >  1:331265/1‑140 (MQ=255)

                                                                                                                    aaCACCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTg            >  2:2099763/1‑140 (MQ=255)

                                                                                                                      cacCGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGc          >  1:945797/1‑140 (MQ=255)

                                                                                                                         cGAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGcaca       >  2:1168380/1‑140 (MQ=255)

                                                                                                                          gAAAACGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaa      >  2:1113812/1‑140 (MQ=255)

                                                                                                                           aaaaCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaa     <  2:728553/140‑1 (MQ=255)

                                                                                                                           aaaaCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaa     >  1:1257964/1‑140 (MQ=255)

                                                                                                                            aaaCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa    <  2:2154174/140‑1 (MQ=255)

                                                                                                                            aaaCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa    <  1:460204/140‑1 (MQ=255)

                                                                                                                              aCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgt  <  2:2006657/140‑1 (MQ=255)

                                                                                                                              aCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgt  <  1:1388118/140‑1 (MQ=255)

                                                                                                                              aCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgt  <  2:2402968/140‑1 (MQ=255)

                                                                                                                              aCGGCTTCAGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGNTATNATTNACGTTATTATTCCGCCGAAACCGGCTTTCNACATTGTTTACTGGCACAAAAgt  <  1:1369804/140‑1 (MQ=255)

ATATAGGGTTTAAGCTGCTCGTAATTTGCTGCGATTAAAATCAGCCCCTTATCAACCGGACGCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGT  >  NC_000913/3431201‑3431466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A