Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F28 I1 R1
|
161 |
50.8 |
1735388 |
98.6% |
1711092 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
867,048 |
+G |
coding (481/966 nt) |
iaaA → |
isoaspartyl dipeptidase proenzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 867,044 | 1 | . | G | 100.0%
| 61.8
/ NA
| 19 | E159E (GAA→GAG) | iaaA | isoaspartyl dipeptidase proenzyme |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/5); total (14/5) |
TGATGGAGCAAAGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAA‑GGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAA > NC_000913/866905‑867172
|
tGATGGAGCAAAGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCaaggaa < 1:36162/140‑1 (MQ=255)
aaaGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAA‑gggggcaac > 2:62402/1‑135 (MQ=255)
aaaGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAA‑gggggcaac < 2:20120/140‑6 (MQ=255)
aaGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAAcc > 1:729422/1‑140 (MQ=255)
aGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCg > 1:710285/1‑140 (MQ=255)
ccGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTcc < 1:362351/140‑1 (MQ=255)
atgatgatTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTc > 2:551842/1‑130 (MQ=255)
atgatgatTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTc < 1:551842/130‑1 (MQ=255)
tgatgatTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAg < 2:710285/140‑1 (MQ=255)
atTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTg < 1:62402/140‑1 (MQ=255)
ttttGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAg > 2:151649/1‑140 (MQ=255)
cgcgTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCTGtct > 1:162352/1‑138 (MQ=255)
cgTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCg > 1:379951/1‑140 (MQ=255)
cgTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCg < 1:26405/140‑1 (MQ=255)
cgTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCg > 2:209679/1‑140 (MQ=255)
gCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGAt > 1:194199/1‑140 (MQ=255)
tGAACAACTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTcc > 2:124419/1‑140 (MQ=255)
aacTACTGGCAGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCGATTTGGCGGCAGCCACGTCCACAgg > 1:198083/1‑140 (MQ=255)
aGCGCGCAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGAcc > 1:380805/1‑140 (MQ=255)
cgcgcAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCaa > 1:774353/1‑140 (MQ=255)
cgcgcAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCaa > 1:784395/1‑140 (MQ=255)
cgcgcAAGGAAGGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCaa > 1:157909/1‑140 (MQ=255)
|
TGATGGAGCAAAGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAA‑GGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAA > NC_000913/866905‑867172
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
