breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 15,094 C→T G309G (GGC→GGT dnaJ → chaperone protein DnaJ
RA 40,223 A→G L55P (CTG→CCG)  caiA ← crotonobetainyl‑CoA reductase
RA 40,677 T→C T419A (ACC→GCC)  caiT ← L‑carnitine:gamma‑butyrobetaine antiporter
RA 77,267 G→T I11I (ATC→ATA sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 92,843 A→G P477P (CCA→CCG ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 131,055 A→G V69A (GTC→GCC)  yacH ← DUF3300 domain‑containing protein YacH
RA 161,232 A→G A85A (GCT→GCC sfsA ← putative DNA‑binding transcriptional regulator of maltose metabolism
RA 255,174 (C)6→7 coding (543/1458 nt) pepD ← peptidase D
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 271,774 A→G pseudogene (11/427 nt) insN → CP4‑6 prophage; IS911A regulator fragment
RA 282,445 C→T L56L (CTC→CTT yagE → CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase
RA 377,726 C→T V215I (GTT→ATT)  frmB ← S‑formylglutathione hydrolase FrmB
RA 380,022 (G)10→11 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 399,700 Δ1 bp coding (108/213 nt) yaiZ → DUF2754 domain‑containing protein YaiZ
RA 445,979 A→G S230P (TCG→CCG)  yajR ← putative major facilitator superfamily transport protein YajR
RA 498,514 C→T P154S (CCG→TCG)  hemH → ferrochelatase
RA 504,214 A→G S161S (AGT→AGC fsr ← fosmidomycin efflux pump
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 548,612 (G)5→6 coding (256/1260 nt) ylbE → DUF1116 domain‑containing protein YlbE
RA 614,720 A→G E188E (GAA→GAG entF → apo‑serine activating enzyme
RA 676,210 (G)5→4 coding (338/978 nt) ybeQ ← Sel1 repeat‑containing protein YbeQ
RA 695,876 A→G Y259C (TAC→TGC)  ubiF → 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
RA 867,048 +G coding (481/966 nt) iaaA → isoaspartyl dipeptidase proenzyme
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 915,225 (C)5→4 intergenic (‑368/+127) lysO ← / ← aqpZ L‑lysine exporter/water channel AqpZ
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 979,025 A→G E900E (GAA→GAG mukB → chromosome partitioning protein MukB
RA 1,001,379 A→G T722A (ACC→GCC)  elfC → putative fimbrial usher protein ElfC
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,024,413 C→T intergenic (‑65/‑58) yccF ← / → helD conserved inner membrane protein YccF/DNA helicase IV
RA 1,047,286 C→T L220L (CTG→CTA gfcD ← putative lipoprotein GfcD
RA 1,055,516 C→T G221G (GGG→GGA torS ← sensory histidine kinase TorS
RA 1,059,758 A→G N168S (AAC→AGC)  torA → trimethylamine N‑oxide reductase 1
RA 1,062,267 (A)6→7 coding (469/600 nt) torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,098,278 A→G E131E (GAA→GAG ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,110,359 A→G E342G (GAG→GGG)  opgG → osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
RA 1,112,805 A→G D648G (GAC→GGC)  opgH → osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
RA 1,129,255 A→G T473A (ACC→GCC)  murJ → putative lipid II flippase MurJ
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,195,548 A→G K142K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,226,987 T→C intergenic (+19/+84) ycgJ → / ← pliG PF05666 family protein YcgJ/inhibitor of g‑type lysozyme
RA 1,288,004 T→C E207E (GAA→GAG purU ← formyltetrahydrofolate deformylase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,358,625 A→G V188A (GTT→GCT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,450,841 G→A A170V (GCG→GTG)  tynA ← copper‑containing amine oxidase
RA 1,539,241 A→G V1117A (GTC→GCC)  narZ ← nitrate reductase Z subunit alpha
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,653,548 A→G V123A (GTT→GCT)  rspB ← putative zinc‑binding dehydrogenase RspB
RA 1,673,621 C→T intergenic (‑120/‑292) mdtJ ← / → tqsA multidrug/spermidine efflux pump membrane subunit MdtJ/autoinducer 2 exporter
RA 1,684,373 T→C F39L (TTC→CTC)  tus → DNA replication terminus site‑binding protein
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,701,278 T→C S108P (TCT→CCT)  malY → negative regulator of MalT activity/cystathionine beta‑lyase
RA 1,817,080 T→C N19S (AAC→AGC)  chbG ← chitin disaccharide deacetylase
RA 1,831,209 G→A F258F (TTC→TTT astC ← succinylornithine transaminase
RA 1,841,780 A→G E97E (GAA→GAG nudG → 5‑hydroxy‑CTP diphosphatase
RA 1,869,433 T→C V160A (GTT→GCT)  yeaH → DUF444 domain‑containing protein YeaH
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,917,976 T→C L156P (CTG→CCG)  yebT → intermembrane transport protein YebT
RA 1,941,225 T→C Q137Q (CAA→CAG mepM ← peptidoglycan DD‑endopeptidase MepM
RA 1,945,088 A→G V90A (GTG→GCG)  ruvB ← Holliday junction branch migration complex subunit RuvB
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,987,451 G→A intergenic (+23/+56) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 1,987,851 (A)7→6 intergenic (‑93/+22) yecJ ← / ← azuC DUF2766 domain‑containing protein YecJ/uncharacterized protein AzuC
RA 2,065,685 A→G V27A (GTT→GCT)  cobU ← cobinamide‑P guanylyltransferase/cobinamide kinase
RA 2,070,778 T→C intergenic (+304/‑8) yoeA → / → yeeP CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA/CP4‑44 prophage; putative GTP‑binding protein
RA 2,114,879 G→A Q340* (CAA→TAA)  wcaM ← putative colanic acid biosynthesis protein WcaM
RA 2,143,684 (G)6→7 coding (419/3318 nt) dgcE → putative diguanylate cyclase DgcE
RA 2,152,125 T→C G116G (GGA→GGG pphC ← protein‑serine/threonine phosphatase PphC
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,237,246 T→C T170A (ACC→GCC)  mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
JC 2,288,561 (C)7→10 pseudogene (354/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,320,920 T→C V293A (GTT→GCT)  atoS → sensory histidine kinase AtoS
RA 2,337,694 T→C D576G (GAC→GGC)  gyrA ← DNA gyrase subunit A
RA 2,403,371 T→C T159A (ACC→GCC)  nuoC ← NADH:quinone oxidoreductase subunit CD
RA 2,412,094 G→A intergenic (‑5/+6) hxpA ← / ← yfbU hexitol phosphatase A/UPF0304 family protein YfbU
RA 2,421,441 C→T H39H (CAC→CAT folX → dihydroneopterin triphosphate 2'‑epimerase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,474,724 T→C R231R (CGT→CGC yfdQ → CPS‑53 (KpLE1) prophage; protein YfdQ
RA 2,492,953 G→C A101G (GCC→GGC)  frc ← formyl‑CoA transferase
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,625,316 T→C C68R (TGT→CGT)  ppx → exopolyphosphatase
RA 2,729,374 T→C noncoding (71/76 nt) gltW ← tRNA‑Glu
RA 2,761,279 A→G intergenic (+106/+72) yfjJ → / ← abpB CP4‑57 prophage; protein YfjJ/CP4‑57 prophage; putative helicase YfjK
RA 2,780,452 G→A R759C (CGC→TGC)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,788,119 G→A pseudogene (1723/2253 nt) ygaQ → putative uncharacterized protein YgaQ
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,833,153 C→T S226S (TCC→TCT norV → anaerobic nitric oxide reductase flavorubredoxin
RA 2,846,372 T→C T34A (ACT→GCT)  hycE ← formate hydrogenlyase subunit HycE
RA 2,849,612 T→C S80G (AGC→GGC)  hycB ← formate hydrogenlyase subunit HycB
RA 2,853,794 Δ1 bp coding (541/1011 nt) hypE → hydrogenase maturation protein, carbamoyl dehydratase
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,868,295 T→C A153A (GCA→GCG nlpD ← murein hydrolase activator NlpD
RA 2,876,427 T→C intergenic (‑98/‑154) cysD ← / → iap sulfate adenylyltransferase subunit 2/alkaline phosphatase isozyme conversion protein
RA 2,912,591 T→C H354R (CAC→CGC)  relA ← GDP/GTP pyrophosphokinase
RA 2,937,348 T→C Y589H (TAC→CAC)  fucI → L‑fucose isomerase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,968,380 T→C N19D (AAT→GAT)  ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 3,001,304 (G)7→8 coding (960/2259 nt) xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,037,280 (T)5→6 coding (827/1734 nt) recJ ← ssDNA‑specific exonuclease RecJ
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,184,476 (A)7→8 intergenic (‑10/‑364) ribB ← / → ubiK 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK
RA 3,192,056 T→A intergenic (‑117/‑152) glgS ← / → yqiJ surface composition regulator/putative inner membrane protein
RA 3,208,763 T→C V247A (GTT→GCT)  ttdT → L‑tartrate:succinate antiporter
RA 3,210,770 G→A intergenic (‑227/‑11) tsaD ← / → rpsU N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit/30S ribosomal subunit protein S21
RA 3,245,976 G→A P251P (CCG→CCA exuT → hexuronate transporter
RA 3,247,296 G→A W215* (TGG→TGA exuR → DNA‑binding transcriptional repressor ExuR
RA 3,273,700 C→T G403D (GGC→GAC)  garP ← galactarate/glucarate/glycerate transporter GarP
RA 3,304,813 (C)7→9 coding (1005/1245 nt) mtr ← tryptophan:H(+) symporter Mtr
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,369,301 (T)6→7 coding (178/465 nt) yhcH ← DUF386 domain‑containing protein YhcH
RA 3,369,628 G→A G241G (GGC→GGT nanK ← N‑acetylmannosamine kinase
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,413,892 T→C L10P (CTG→CCG)  acrE → multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,486,874 C→T P28P (CCC→CCT yhfK → putative transporter YhfK
RA 3,495,261 A→G K417K (AAA→AAG nirB → nitrite reductase catalytic subunit NirB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,574,756 G→A G41G (GGC→GGT asd ← aspartate‑semialdehyde dehydrogenase
RA 3,590,205 G→A A236V (GCC→GTC)  ugpA ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
RA 3,614,979 A→G H438R (CAC→CGC)  nikA → Ni(2(+)) ABC transporter periplasmic binding protein
RA 3,712,106 A→G intergenic (‑99/+130) yhjX ← / ← yhjY putative pyruvate transporter/conserved protein YhjY
RA 3,739,214 (G)5→6 coding (1718/2031 nt) malS → alpha‑amylase
RA 3,778,503 T→C F369L (TTC→CTC)  lldP → lactate/glycolate:H(+) symporter LldP
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,926,533 T→C intergenic (‑78/+12) mioC ← / ← asnC flavoprotein MioC/DNA‑binding transcriptional dual regulator AsnC
RA 3,976,924 A→G Q216R (CAG→CGG)  wzxE → lipid III flippase
RA 4,091,820 G→A L183F (CTT→TTT)  frvB ← putative PTS enzyme IIBC component FrvB
RA 4,108,056 G→C V169L (GTG→CTG)  pfkA → 6‑phosphofructokinase I
RA 4,226,907 A→G H1027R (CAC→CGC)  metH → cobalamin‑dependent methionine synthase
RA 4,241,093 C→T G388S (GGT→AGT)  xylE ← D‑xylose:H(+) symporter
RA 4,255,072 A→G G465G (GGT→GGC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,258,984 A→G T415A (ACG→GCG)  dinF → DNA damage‑inducible protein F
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,328,006 C→T P291L (CCG→CTG)  crfC → clamp‑binding sister replication fork colocalization protein
RA 4,346,487 G→A A280A (GCC→GCT fumB ← fumarase B
RA 4,396,661 A→G G199G (GGA→GGG amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,400,926 C→A S85R (AGC→AGA hflX → ribosome rescue factor HflX
RA 4,405,247 T→C V187V (GTT→GTC purA → adenylosuccinate synthetase
RA 4,411,727 A→G E142E (GAA→GAG yjfK → conserved protein YjfK
RA 4,415,730 A→G T486A (ACT→GCT)  aidB → putative acyl‑CoA dehydrogenase AidB
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,474,275 (C)6→7 coding (152/594 nt) bdcR → putative transcriptional regulator BdcR
RA 4,485,191 T→C Y254C (TAC→TGC)  pepA ← aminopeptidase A/I
RA 4,488,579 (C)7→8 coding (1485/1503 nt) yjgR ← DUF853 domain‑containing protein YjgR
RA 4,530,092 A→G V51A (GTC→GCC)  sgcC ← putative PTS enzyme IIC component SgcC
RA 4,553,532 C→T A211V (GCG→GTG)  uxuB → D‑mannonate oxidoreductase
RA 4,556,769 (T)9→8 coding (116/144 nt) ytiD → protein YtiD
RA 4,576,245 T→A pseudogene (2111/2721 nt) yjiV → putative uncharacterized protein YjiV
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,636,147 A→G V47V (GTA→GTG creB → DNA‑binding transcriptional regulator CreB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196287 1211431 15145 13 [11] [10] 12 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1633064–1633220 1633816–1633634 415–753 13 [9] [11] 12 ydfK–[pinQ] ydfK,[pinQ]
* * ÷ NC_000913 3423768–3424234 3424536–3424238 5–769 12 [11] [11] 16 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 340035 =NA (NA)47 (0.940) 17/274 1.7 NA coding (243/297 nt) yahH putative uncharacterized protein YahH
?NC_000913 377322 = NA (NA)intergenic (+11/+213) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 374922 =NA (NA)21 (0.570) 11/202 1.8 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377430 = NA (NA)intergenic (+119/+105) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)37 (1.070) 13/190 1.3 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375118NA (NA)19 (0.480) 11/216 2.0 NA noncoding (1/36 nt) other REP31d
?NC_000913 = 377333 NA (NA)intergenic (+22/+202) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375160NA (NA)26 (0.520)
+T
14/274 2.1 NA noncoding (28/34 nt) other REP31e
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 = 375165NA (NA)7 (0.200) 6/192 2.9 NA noncoding (33/34 nt) other REP31e
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375219NA (NA)24 (0.610) 19/216 0.9 NA noncoding (1/36 nt) other REP31f
?NC_000913 = 377333 NA (NA)intergenic (+22/+202) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375246NA (NA)14 (0.310)
+GAACGCCTTATCCGGT
10/244 2.5 NA noncoding (13/34 nt) other REP31g
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 377321 =NA (NA)31 (0.620) 14/274 2.1 NA intergenic (+10/+214) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
?NC_000913 4325851 = NA (NA)intergenic (‑110/+548) yjdN/yjdM conserved protein YjdN/conserved protein YjdM
* ? NC_000913 608007 =NA (NA)20 (0.490) 8/224 2.8 36.4% noncoding (1/1345 nt) IS186 repeat region
?NC_000913 609352 = 35 (0.850)intergenic (+175/+107) insL‑2/entD IS186/IS421 transposase/phosphopantetheinyl transferase EntD
* ? NC_000913 3363001 =86 (1.700)74 (1.470) 47/274 0.1 63.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)69 (1.370) 47/276 0.1 98.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA