Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F28 I1 R1
|
161 |
50.8 |
1735388 |
98.6% |
1711092 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,024,413 |
C→T |
intergenic (‑65/‑58) |
yccF ← / → helD |
conserved inner membrane protein YccF/DNA helicase IV |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,024,413 | 0 | C | T | 94.6%
| 111.8
/ ‑1.7
| 37 | intergenic (‑65/‑58) | yccF/helD | conserved inner membrane protein YccF/DNA helicase IV |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (13/22); total (14/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 |
TCGCCAACAGCCAGCCCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGCACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAGCACCCTTACGATCGGGCGGTGATCCTCAATGC > NC_000913/1024276‑1024544
|
tCGCCAACAGCCAGCCCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTAc > 2:581151/1‑140 (MQ=255)
ccAACAGCCAGCCCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGtt < 1:192185/140‑1 (MQ=255)
ccAACAGCCAGCCCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGtt < 1:662237/140‑1 (MQ=255)
gccagccCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct < 2:190489/140‑1 (MQ=255)
gccagccCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct < 2:865168/140‑1 (MQ=255)
gccagccCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct < 2:726674/140‑1 (MQ=255)
gccagccCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct < 2:490238/140‑1 (MQ=255)
gccagccCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct < 2:40560/140‑1 (MQ=255)
gccagccCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct < 2:155853/140‑1 (MQ=255)
gccagccCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct < 1:281565/140‑1 (MQ=255)
agTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTaca < 1:91725/140‑1 (MQ=255)
gtggtgGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct > 1:195892/1‑129 (MQ=255)
gtggtgGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTctct < 2:195892/129‑1 (MQ=255)
gtggtgGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTacac < 1:536477/140‑1 (MQ=255)
tggCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTaca < 2:697560/135‑1 (MQ=255)
tggCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTaca > 1:697560/1‑135 (MQ=255)
tggCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGAt > 1:6463/1‑140 (MQ=255)
ggCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATa < 2:667525/140‑1 (MQ=255)
cacaAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCgtg < 2:626471/140‑1 (MQ=255)
aGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACa > 1:571/1‑140 (MQ=255)
aGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGCACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACa > 1:824316/1‑140 (MQ=255)
tCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCAt > 2:777741/1‑140 (MQ=255)
aaCGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGaa < 2:238925/140‑1 (MQ=255)
ggTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTg > 1:406391/1‑140 (MQ=255)
tACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGaa > 1:508933/1‑140 (MQ=255)
aCGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGaaa > 1:695023/1‑140 (MQ=255)
aaaCCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGCACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACaa < 2:824316/140‑1 (MQ=255)
cTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCt > 1:379626/1‑140 (MQ=255)
tACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGaaaa > 2:718624/1‑140 (MQ=255)
cTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTAGAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACg > 2:726286/1‑140 (MQ=255)
tttCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGca < 1:644635/140‑1 (MQ=255)
cAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAg < 2:6463/140‑1 (MQ=255)
aGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAGc < 1:777741/140‑1 (MQ=255)
ggcggcAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAg > 2:245285/1‑132 (MQ=255)
ggcggcAATTGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAg < 1:245285/132‑1 (MQ=255)
ttGTAACGTTTTTTTGGGCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAGCACCCTTACGATCggg < 2:379626/140‑1 (MQ=255)
ggCTGGAGTACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAGCACCCTTACGATCGGGCGGTGATCCTCAATGc < 2:508933/140‑1 (MQ=255)
|
TCGCCAACAGCCAGCCCAGAGTGGTGGCAAATCCGCCAAGCACAAAGTTCAGAATGTTCAAAACGGTACGCATAAAACCTCGCTTTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGCACGTTTTCTCTGACGGTTACACTGATAAGAAATAATTTCGTGTGGATCTACAGAGTCATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAGCACCCTTACGATCGGGCGGTGATCCTCAATGC > NC_000913/1024276‑1024544
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
