Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 161 50.8 1735388 98.6% 1711092 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,062,267 (A)6→7 coding (469/600 nt) torD → trimethylamine‑N‑oxide reductase‑specific chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,062,2611.A100.0% 131.5 / NA 38Q155K (CAA→AAA) torDtrimethylamine‑N‑oxide reductase‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (18/20);  total (18/20)

GCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGC‑AAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTGGTGTTAGTGGAGTGCGACCACCAAAACA  >  NC_000913/1062129‑1062393
                                                                                                                                     |                                                                                                                                    
gCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGC‑aaaaaaa                                                                                                                               >  1:778517/1‑139 (MQ=255)
gCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGC‑aaaaaaa                                                                                                                               >  1:715211/1‑139 (MQ=255)
         gAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACgg                                                                                                                       <  2:457157/140‑1 (MQ=255)
            aCCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGgcgc                                                                                                                    <  2:461271/140‑1 (MQ=255)
            aCCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGgcgc                                                                                                                    <  1:810486/140‑1 (MQ=255)
            aCCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGgcgc                                                                                                                    <  1:726114/140‑1 (MQ=255)
                 cGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTgg                                                                                                               <  2:778517/140‑1 (MQ=255)
                   gCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCa                                                                                                             <  1:283227/140‑1 (MQ=255)
                   gCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCa                                                                                                             <  2:263305/140‑1 (MQ=255)
                     aaTTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAAt                                                                                                           <  2:810397/140‑1 (MQ=255)
                                            tcTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGt                                                                                    >  1:11833/1‑140 (MQ=255)
                                              tGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGt                                                                                    <  1:185021/138‑1 (MQ=255)
                                              tGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGt                                                                                    >  2:185021/1‑138 (MQ=255)
                                                  gATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgtcgt                                                                              <  2:715211/140‑1 (MQ=255)
                                                   atctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGTCAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgtcgtc                                                                             >  1:404817/1‑140 (MQ=255)
                                                              aTTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGc                                                                  >  1:444143/1‑140 (MQ=255)
                                                               ttGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCt                                                                 <  2:11833/140‑1 (MQ=255)
                                                                  cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTg                                                              >  1:351129/1‑140 (MQ=255)
                                                                  cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTg                                                              >  2:428522/1‑140 (MQ=255)
                                                                  cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTg                                                              >  1:584913/1‑140 (MQ=255)
                                                                  cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTg                                                              >  1:393299/1‑140 (MQ=255)
                                                                  cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTAGGACAGCTTTg                                                              >  2:532467/1‑140 (MQ=255)
                                                                    cAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGt                                                            <  2:351129/140‑1 (MQ=255)
                                                                          tCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTAc                                                      >  1:427173/1‑140 (MQ=255)
                                                                          tCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTAc                                                      >  2:400334/1‑140 (MQ=255)
                                                                           cTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTAcg                                                     <  1:400334/140‑1 (MQ=255)
                                                                           cTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTAcg                                                     <  1:428436/140‑1 (MQ=255)
                                                                            tGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTAcgc                                                    >  2:780198/1‑140 (MQ=255)
                                                                                   ttCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCa                                                <  2:504980/137‑1 (MQ=255)
                                                                                   ttCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCa                                                >  1:504980/1‑137 (MQ=255)
                                                                                     cGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAg                                           >  2:130583/1‑140 (MQ=255)
                                                                                        tGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCa                                        >  1:854145/1‑140 (MQ=255)
                                                                                               ggggACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTg                                 <  1:780198/140‑1 (MQ=255)
                                                                                                   aCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTgg                             <  2:444143/140‑1 (MQ=255)
                                                                                                      gTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTGgtgt                          >  2:823356/1‑140 (MQ=255)
                                                                                                          cTGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTGGTGTTAGt                      >  1:777045/1‑140 (MQ=255)
                                                                                                           tGCGCGAAGAATCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTGGTGTTAGTg                     <  1:823356/140‑1 (MQ=255)
                                                                                                                    aaTCGACAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTGGTGTTAGTGGAGTGCGac            <  1:639504/140‑1 (MQ=255)
                                                                                                                       cgtcAGTTTGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTGGTGTTAGTGGAGTGCGaccac         <  2:404817/137‑1 (MQ=255)
                                                                                                                              ttGCGGCAAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTGGTGTTAGTGGAGTGCGACCACCAAAACa  >  1:255230/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGC‑AAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGCCAGTTATTGCTGGTGTTAGTGGAGTGCGACCACCAAAACA  >  NC_000913/1062129‑1062393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A