Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	140	59.4	2004938	99.3%	1990903	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	909,556	A→G	G498G (GGT→GGC)	poxB ←	pyruvate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,556	0	A	G	100.0%	188.6 / NA	53	G498G (GGT→GGC)	poxB	pyruvate oxidase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/24); total (29/24)

TTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCA  >  NC_000913/909419‑909692

ttGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGcc                                                                                                                                        >  2:373751/1‑140 (MQ=255)

ttGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGcc                                                                                                                                        >  2:295046/1‑140 (MQ=255)

       gTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATg                                                                                                                                 >  1:402761/1‑140 (MQ=255)

        ttCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGc                                                                                                                                >  1:843058/1‑140 (MQ=255)

          cGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGTGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCg                                                                                                                              >  2:684234/1‑140 (MQ=255)

          cGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCg                                                                                                                              <  2:360955/140‑1 (MQ=255)

          cGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCg                                                                                                                              <  2:402761/140‑1 (MQ=255)

            aGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGTACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCa                                                                                                                            <  1:689565/140‑1 (MQ=255)

              tttGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAGCACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACg                                                                                                                          <  1:145267/140‑1 (MQ=255)

                  aTCTGCGGTGGAATGGCTAACTCTCCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTc                                                                                                                      >  1:824836/1‑140 (MQ=255)

                       cGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCaa                                                                                                                 >  1:724383/1‑140 (MQ=255)

                              aTGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGc                                                                                                          <  1:653702/140‑1 (MQ=255)

                                    aaCTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGtt                                                                                                    >  1:900739/1‑140 (MQ=255)

                                       tcttctTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTtgt                                                                                                 <  1:337352/140‑1 (MQ=255)

                                        cttcttTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTtgtg                                                                                                <  2:724383/140‑1 (MQ=255)

                                        cttcttTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTtgtg                                                                                                <  1:902659/140‑1 (MQ=255)

                                          tcttTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTc                                                                                              >  1:819156/1‑140 (MQ=255)

                                             ttGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGTGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCgtg                                                                                           <  1:684234/140‑1 (MQ=255)

                                                 cGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGt                                                                                       >  2:137079/1‑140 (MQ=255)

                                                      accacATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGt                                                                                  >  2:987387/1‑140 (MQ=255)

                                                           aTCCACCAACACCGGACCGTCGATAGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgt                                                                             <  1:911676/140‑1 (MQ=255)

                                                              caccaACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgtcag                                                                          >  2:649087/1‑140 (MQ=255)

                                                                  aaCACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCaa                                                                      >  2:405680/1‑140 (MQ=255)

                                                                     acCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATa                                                                   >  1:484983/1‑140 (MQ=255)

                                                                     acCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATa                                                                   <  1:446692/140‑1 (MQ=255)

                                                                     acCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATa                                                                   <  2:824836/140‑1 (MQ=255)

                                                                       cGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGc                                                                 >  2:546780/1‑140 (MQ=255)

                                                                         gACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGcca                                                               >  1:119461/1‑140 (MQ=255)

                                                                          aCCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGccac                                                              >  1:567378/1‑140 (MQ=255)

                                                                             gTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAg                                                           <  1:649087/140‑1 (MQ=255)

                                                                              tCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAg                                                           <  2:870252/139‑1 (MQ=255)

                                                                              tCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAg                                                           >  1:870252/1‑139 (MQ=255)

                                                                              tCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGc                                                          >  2:567803/1‑140 (MQ=255)

                                                                                  tGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTc                                                      <  2:119461/140‑1 (MQ=255)

                                                                                   ggAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCa                                                     >  1:665660/1‑140 (MQ=255)

                                                                                        aGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTcc                                                >  2:544676/1‑140 (MQ=255)

                                                                                          gcgcGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCAt                                              <  1:655820/140‑1 (MQ=255)

                                                                                          gcgcGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCAt                                              >  2:655820/1‑140 (MQ=255)

                                                                                                 gCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCcaca                                       <  2:567378/140‑1 (MQ=255)

                                                                                                 gCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCcaca                                       <  1:235785/140‑1 (MQ=255)

                                                                                                   aGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACaaa                                     >  2:423831/1‑140 (MQ=255)

                                                                                                          catcaACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGc                              >  2:528674/1‑140 (MQ=255)

                                                                                                          catcaACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGc                              >  1:573329/1‑140 (MQ=255)

                                                                                                           atcaACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCa                             <  1:546780/140‑1 (MQ=255)

                                                                                                             caACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACg                           <  2:484983/140‑1 (MQ=255)

                                                                                                             caACTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACg                           <  1:528674/140‑1 (MQ=255)

                                                                                                              aaCTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGTACGc                          <  2:665660/140‑1 (MQ=255)

                                                                                                              aaCTTCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGc                          <  2:573329/140‑1 (MQ=255)

                                                                                                                 ttCAGACGCTTTTTCTACACGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCtgt                       >  1:426814/1‑140 (MQ=255)

                                                                                                                                  cacGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAtt      >  1:235013/1‑140 (MQ=255)

                                                                                                                                    cGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAtttt    >  1:602593/1‑140 (MQ=255)

                                                                                                                                    cGGATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAtttt    >  1:228737/1‑140 (MQ=255)

                                                                                                                                      gATGCCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCa  <  1:137079/140‑1 (MQ=255)

TTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCA  >  NC_000913/909419‑909692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A