Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	140	59.4	2004938	99.3%	1990903	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,195,769	A→G	Y216C (TAC→TGC)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,195,769	0	A	G	100.0%	179.1 / NA	51	Y216C (TAC→TGC)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/22); total (29/22)

CGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGATCGACGG  >  NC_000913/1195632‑1195901

cGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCg                                                                                                                                    <  1:649394/140‑1 (MQ=255)

    gCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAAt                                                                                                                                >  2:778262/1‑140 (MQ=255)

         gaAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTa                                                                                                                           <  1:20310/140‑1 (MQ=255)

         gaAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTa                                                                                                                           >  2:531893/1‑140 (MQ=255)

          aaaGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTaa                                                                                                                          <  1:783310/140‑1 (MQ=255)

           aaGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAAc                                                                                                                         <  1:256092/140‑1 (MQ=255)

              tGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGAt                                                                                                                      >  2:921000/1‑140 (MQ=255)

              tGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGAt                                                                                                                      >  2:293684/1‑140 (MQ=255)

                  tAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCgtg                                                                                                                  >  1:275157/1‑140 (MQ=255)

                        ccTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCgtgactctg                                                                                                            >  1:770183/1‑140 (MQ=255)

                             gTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCgtgactctgtgact                                                                                                       <  1:293684/140‑1 (MQ=255)

                             gTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCgtgactctgtgact                                                                                                       <  1:900295/140‑1 (MQ=255)

                                     aTGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGca                                                                                               >  1:362221/1‑140 (MQ=255)

                                      tGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGcac                                                                                              >  1:852766/1‑140 (MQ=255)

                                       gggggTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGcaca                                                                                             >  2:299914/1‑140 (MQ=255)

                                          ggTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAg                                                                                          >  1:560994/1‑140 (MQ=255)

                                           gTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAgg                                                                                         >  1:916416/1‑140 (MQ=255)

                                           gTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAgg                                                                                         <  2:560994/140‑1 (MQ=255)

                                            tGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGTCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTC‑‑TGACTCTGGTGCACAAAGGCaa                                                                                      >  1:786275/1‑140 (MQ=255)

                                             gaagaaAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCa                                                                                       <  2:770183/140‑1 (MQ=255)

                                               agaaAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAAc                                                                                     >  2:956594/1‑140 (MQ=255)

                                                                   cATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAgg                                                                 <  1:778262/140‑1 (MQ=255)

                                                                   cATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAgg                                                                 <  2:362221/140‑1 (MQ=255)

                                                                    aTTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGa                                                                <  1:299914/140‑1 (MQ=255)

                                                                     ttGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAg                                                               <  1:956594/140‑1 (MQ=255)

                                                                       gtgGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCg                                                             >  1:776438/1‑140 (MQ=255)

                                                                           tATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTa                                                         >  1:691201/1‑140 (MQ=255)

                                                                           tATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTa                                                         >  2:823709/1‑140 (MQ=255)

                                                                             tCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTaaa                                                       >  2:644946/1‑140 (MQ=255)

                                                                                gTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGAc                                                    >  2:178734/1‑140 (MQ=255)

                                                                                gTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGAc                                                    >  1:327776/1‑140 (MQ=255)

                                                                                 tATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACt                                                   <  1:921000/140‑1 (MQ=255)

                                                                                     aaGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTgggg                                               >  1:424828/1‑140 (MQ=255)

                                                                                        ccGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTa                                            >  1:481774/1‑140 (MQ=255)

                                                                                           tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACTATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCt                                             <  2:870929/136‑1 (MQ=255)

                                                                                           tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACTATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCt                                             >  1:870929/1‑136 (MQ=255)

                                                                                           tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCa                                         >  2:453132/1‑140 (MQ=255)

                                                                                           tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCa                                         >  2:191132/1‑140 (MQ=255)

                                                                                           tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCa                                         >  2:776430/1‑140 (MQ=255)

                                                                                           tgtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCa                                         >  1:568400/1‑140 (MQ=255)

                                                                                            gtTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAg                                        <  2:776438/140‑1 (MQ=255)

                                                                                                ggAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTgg                                    >  1:191086/1‑140 (MQ=255)

                                                                                                  aagaagGCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGgcg                                  <  1:482305/140‑1 (MQ=255)

                                                                                                       ggCACCAAACGTCTGGTTCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGa                             <  1:178734/140‑1 (MQ=255)

                                                                                                                       ttCGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGaa             <  1:342292/140‑1 (MQ=255)

                                                                                                                         cGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCg                  >  2:11244/1‑133 (MQ=255)

                                                                                                                         cGTGCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCg                  <  1:11244/133‑1 (MQ=255)

                                                                                                                            gCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGAt        <  2:691201/140‑1 (MQ=255)

                                                                                                                            gCAGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGAt        <  1:776430/140‑1 (MQ=255)

                                                                                                                              aGCGATCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGATCg      >  2:718474/1‑140 (MQ=255)

                                                                                                                                  aTCGAATGCGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGATCGACgg  <  2:327776/140‑1 (MQ=255)

CGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAACGATCGTGACTCTGTGACTCTGGTGCACAAAGGCAACATCATGAAGTTCACCGAAGGAGCGTTTAAAGACTGGGGCTACCAGCTGGCGCGTGAAGAGTTTGGCGGTGAACTGATCGACGG  >  NC_000913/1195632‑1195901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A