Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	140	59.4	2004938	99.3%	1990903	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,072	A→G	Y81C (TAC→TGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,072	0	A	G	100.0%	164.5 / NA	48	Y81C (TAC→TGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24)

CGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGTCGACCTGG  >  NC_000913/1335939‑1336206

cGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGccc                                                                                                                                  <  1:247289/140‑1 (MQ=255)

   cTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGc                                                                                                                               >  1:137200/1‑140 (MQ=255)

   cTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGc                                                                                                                               >  2:815079/1‑140 (MQ=255)

   cTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGc                                                                                                                               >  1:344533/1‑140 (MQ=255)

     aCCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCaa                                                                                                                             <  1:102276/140‑1 (MQ=255)

     aCCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCaa                                                                                                                             >  1:535342/1‑140 (MQ=255)

      cccAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAg                                                                                                                            <  2:749662/140‑1 (MQ=255)

          aGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTg                                                                                                                        >  2:431014/1‑140 (MQ=255)

            tCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTG‑AAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTg                                                                                                                     >  1:482934/1‑140 (MQ=255)

                         tGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACttt                                                                                                         >  1:867060/1‑140 (MQ=255)

                              gAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCgg                                                                                                    >  2:141181/1‑140 (MQ=255)

                                    ctgctgCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTAcc                                                                                              <  1:431014/140‑1 (MQ=255)

                                    ctgctgCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTAcc                                                                                              <  2:975198/140‑1 (MQ=255)

                                      gctgcGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTg                                                                                            <  1:638334/140‑1 (MQ=255)

                                       ctgcGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGc                                                                                           <  1:56588/140‑1 (MQ=255)

                                       ctgcGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGc                                                                                           <  1:56587/140‑1 (MQ=255)

                                         gcgcTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGACCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCg                                                                                         <  2:179170/140‑1 (MQ=255)

                                            cTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCgttg                                                                                      >  1:675792/1‑140 (MQ=255)

                                                                 tACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGcgcg                                                                 <  2:137200/140‑1 (MQ=255)

                                                                 tACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGcgcg                                                                 >  2:176276/1‑140 (MQ=255)

                                                                 tACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGcgcg                                                                 >  1:416470/1‑140 (MQ=255)

                                                                 tACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGcgcg                                                                 <  2:683263/140‑1 (MQ=255)

                                                                  aCCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGa                                                                <  2:784041/140‑1 (MQ=255)

                                                                  aCCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGa                                                                >  2:41409/1‑140 (MQ=255)

                                                                   ccGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGaa                                                               >  2:740451/1‑140 (MQ=255)

                                                                     gAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGc                                                             <  2:39263/140‑1 (MQ=255)

                                                                      aaGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCg                                                            >  2:957208/1‑140 (MQ=255)

                                                                                  aCGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTc                                                <  2:675792/140‑1 (MQ=255)

                                                                                   cgcgCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGcc                                                  <  1:430639/137‑1 (MQ=255)

                                                                                   cgcgCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGcc                                                  >  2:430639/1‑137 (MQ=255)

                                                                                   cgcgCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTcg                                               >  2:669749/1‑140 (MQ=255)

                                                                                      gcTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTcggcg                                            >  2:405046/1‑140 (MQ=255)

                                                                                        tGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCggcggc                                          >  1:524125/1‑140 (MQ=255)

                                                                                          gCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAAc                                                     <  2:28726/127‑1 (MQ=255)

                                                                                          gCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAAc                                                     >  1:28726/1‑127 (MQ=255)

                                                                                          gCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGa                                        <  1:41409/140‑1 (MQ=255)

                                                                                          gCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGa                                        <  1:176276/140‑1 (MQ=255)

                                                                                                tGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGc                                  <  2:524125/140‑1 (MQ=255)

                                                                                                tGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGc                                  <  2:344533/140‑1 (MQ=255)

                                                                                                tGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGc                                  >  1:719487/1‑140 (MQ=255)

                                                                                                         aaTGCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTaa                         >  2:695091/1‑140 (MQ=255)

                                                                                                            gCCCATGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAAccc                      >  1:612476/1‑140 (MQ=255)

                                                                                                                aTGCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGctc                  >  2:112642/1‑140 (MQ=255)

                                                                                                                  gCTGACCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCtctc                <  2:535342/140‑1 (MQ=255)

                                                                                                                      aCCGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCg            >  2:413659/1‑140 (MQ=255)

                                                                                                                       ccGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCgg           <  2:769177/140‑1 (MQ=255)

                                                                                                                        cGTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGt          <  1:957208/140‑1 (MQ=255)

                                                                                                                         gTGAAATTGCCTGCCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGTc         <  2:719487/140‑1 (MQ=255)

                                                                                                                                tgcctgccGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGTCGACCTgg  <  1:695091/140‑1 (MQ=255)

CGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGCCCGGCAAGGGTGCTGATGCAGGACTTTACCGGCGTACCTGCCGTTGTTGATCTGGCGGCAATGCGCGAAGCGGTTAAACGCCTCGGCGGCGATACTGCAAAGGTTAACCCGCTCTCACCGGTCGACCTGG  >  NC_000913/1335939‑1336206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A