Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	140	77.1	2730754	94.9%	2591485	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	915,074	C→G	intergenic (‑217/+278)	lysO ← / ← aqpZ	L‑lysine exporter/water channel AqpZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	915,074	0	C	G	100.0%	171.0 / NA	54	intergenic (‑217/+278)	lysO/aqpZ	L‑lysine exporter/water channel AqpZ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (27/27); total (27/27)

TAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGG  >  NC_000913/914939‑915207

tAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGcaca                                                                                                                                   <  1:908420/140‑1 (MQ=255)

tAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGcaca                                                                                                                                   <  2:1333372/140‑1 (MQ=255)

    aGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGa                                                                                                                               <  2:669337/140‑1 (MQ=255)

                  aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGgc                                                                                                                 <  1:1204587/140‑1 (MQ=255)

                   tAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGgcg                                                                                                                <  2:183454/140‑1 (MQ=255)

                     aGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAACTCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGcgcg                                                                                                              >  2:1190647/1‑140 (MQ=255)

                     aGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGcgcg                                                                                                              <  2:789379/140‑1 (MQ=255)

                     aGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGcgcg                                                                                                              <  2:1094574/140‑1 (MQ=255)

                      ggAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGt                                                                                                             >  2:618404/1‑140 (MQ=255)

                          cAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTa                                                                                                         >  2:743375/1‑140 (MQ=255)

                           aGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                        >  1:1171196/1‑140 (MQ=255)

                           aGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                        >  1:250862/1‑140 (MQ=255)

                             tAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAg                                                                                                      <  1:743375/140‑1 (MQ=255)

                                agTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGa                                                                                                   >  1:942473/1‑140 (MQ=255)

                                agTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGa                                                                                                   >  1:315594/1‑140 (MQ=255)

                                  tATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAg                                                                                                      >  2:344317/1‑135 (MQ=255)

                                  tATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAg                                                                                                      <  1:344317/135‑1 (MQ=255)

                                    ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGtt                                                                                               >  2:398671/1‑140 (MQ=255)

                                    ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGtt                                                                                               >  1:114648/1‑140 (MQ=255)

                                     tCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                        >  2:21903/1‑130 (MQ=255)

                                     tCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                        <  1:21903/130‑1 (MQ=255)

                                                 aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGa                                                                                  <  1:398671/140‑1 (MQ=255)

                                                 aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGa                                                                                  <  2:1023583/140‑1 (MQ=255)

                                                 aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGa                                                                                  >  1:1023583/1‑140 (MQ=255)

                                                      tatTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCgg                                                                             <  1:1039567/140‑1 (MQ=255)

                                                       atTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGt                                                                            <  2:250862/140‑1 (MQ=255)

                                                             gcgcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAg                                                                          <  2:929248/136‑1 (MQ=255)

                                                             gcgcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAg                                                                          >  1:929248/1‑136 (MQ=255)

                                                               gcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTaa                                                                    <  1:960566/140‑1 (MQ=255)

                                                                cTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACAAGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAg                                                                   >  2:719797/1‑140 (MQ=255)

                                                                   tAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGa                                                                >  1:1038293/1‑140 (MQ=255)

                                                                    aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                               <  2:114648/140‑1 (MQ=255)

                                                                                cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCa                                                   >  1:980965/1‑140 (MQ=255)

                                                                                cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCa                                                   >  2:732895/1‑140 (MQ=255)

                                                                                cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCa                                                   <  1:732895/140‑1 (MQ=255)

                                                                                 aCCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                               <  1:792684/127‑1 (MQ=255)

                                                                                 aCCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                               >  2:792684/1‑127 (MQ=255)

                                                                                        ttGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCgcgc                                             >  1:607015/1‑138 (MQ=25)

                                                                                        ttGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCgcgc                                             <  2:607015/138‑1 (MQ=25)

                                                                                             ggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggcg                                      >  1:406305/1‑140 (MQ=25)

                                                                                             ggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggcg                                      >  1:272399/1‑140 (MQ=21)

                                                                                             ggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggcg                                      >  2:366944/1‑140 (MQ=25)

                                                                                                          ccGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                         <  1:366944/140‑1 (MQ=17)

                                                                                                          ccGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                         >  1:341043/1‑140 (MQ=17)

                                                                                                          ccGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                         <  2:1171196/140‑1 (MQ=17)

                                                                                                          ccGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTAGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                         <  1:1190647/140‑1 (MQ=17)

                                                                                                                    cccTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCgggg               <  1:242839/140‑1 (MQ=17)

                                                                                                                      cTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGATGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGt             <  1:180123/140‑1 (MQ=17)

                                                                                                                       tGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTg            >  1:1099332/1‑140 (MQ=17)

                                                                                                                       tGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTg            >  1:881455/1‑140 (MQ=17)

                                                                                                                              cGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCg                  <  2:1216723/127‑1 (MQ=17)

                                                                                                                              cGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCg                  >  1:1216723/1‑127 (MQ=17)

                                                                                                                               ggggCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggc    >  2:49160/1‑140 (MQ=17)

                                                                                                                                 ggCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcgg  <  2:517962/140‑1 (MQ=17)

TAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGG  >  NC_000913/914939‑915207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A