Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	140	77.1	2730754	94.9%	2591485	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	916,237	A→G	intergenic (‑190/‑236)	aqpZ ← / → ybjD	water channel AqpZ/DUF2813 domain‑containing protein YbjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	916,237	0	A	G	100.0%	163.8 / NA	46	intergenic (‑190/‑236)	aqpZ/ybjD	water channel AqpZ/DUF2813 domain‑containing protein YbjD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/17); total (29/17)

CGTCGTAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGACAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCATTTTT  >  NC_000913/916101‑916370

cgtcgtAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCa                                                                                                                                     >  1:1205754/1‑139 (MQ=255)

  tcgtAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAgtg                                                                                                                                  >  2:890339/1‑140 (MQ=255)

  tcgtAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAgtg                                                                                                                                  >  1:1150066/1‑140 (MQ=255)

  tcgtAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAgtg                                                                                                                                  >  1:138110/1‑140 (MQ=255)

       aaaaaGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATTAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTg                                                                                                                             <  1:674503/140‑1 (MQ=255)

               acgacgGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCaaa                                                                                                                     <  1:1349409/140‑1 (MQ=255)

                      aaaTAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTa                                                                                                              <  2:802737/140‑1 (MQ=255)

                                cgGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAgg                                                                                                    >  2:1335411/1‑140 (MQ=255)

                                cgGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAgg                                                                                                    >  1:604250/1‑140 (MQ=255)

                                cgGCTATTTTAAAAACGAAGGCGAGTCATTCA‑CAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGc                                                                                                   >  2:875766/1‑140 (MQ=255)

                                  gCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCg                                                                                                  <  2:870855/140‑1 (MQ=255)

                                      ttttAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGtaat                                                                                              >  2:650004/1‑140 (MQ=255)

                                                gAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAaataat                                                                                    >  2:1117189/1‑140 (MQ=255)

                                                    gCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTAt                                                                                <  1:1335411/140‑1 (MQ=255)

                                                     cGAGTCATTCA‑CAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCa                                                                              <  1:875766/140‑1 (MQ=255)

                                                                 cAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAattatt                                                                   <  2:604250/140‑1 (MQ=255)

                                                                    ataaataaATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGa                                                                >  2:657202/1‑140 (MQ=255)

                                                                    ataaataaATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGa                                                                <  1:650004/140‑1 (MQ=255)

                                                                     taaataaaTCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGAt                                                               >  2:1032113/1‑140 (MQ=255)

                                                                        ataaatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGAtttt                                                            >  1:1263496/1‑140 (MQ=255)

                                                                        ataaatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGAtttt                                                            >  2:563510/1‑140 (MQ=255)

                                                                         taaatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTttg                                                           <  1:563510/140‑1 (MQ=255)

                                                                         taaatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTttg                                                           >  2:501812/1‑140 (MQ=255)

                                                                           aatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTttgtt                                                         >  1:715240/1‑140 (MQ=255)

                                                                             tCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTtgttgt                                                       >  1:478286/1‑140 (MQ=255)

                                                                             tCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTtgttgt                                                       >  1:1115480/1‑140 (MQ=255)

                                                                                  tAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTg                                                  >  2:111203/1‑140 (MQ=255)

                                                                                    aaTTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCa                                                <  1:1117189/140‑1 (MQ=255)

                                                                                      tttGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGa                                              <  1:364502/140‑1 (MQ=255)

                                                                                       ttGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGAc                                             <  1:890339/140‑1 (MQ=255)

                                                                                                ggCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCa                                    <  1:612079/140‑1 (MQ=255)

                                                                                                ggCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCa                                    <  1:1032113/140‑1 (MQ=255)

                                                                                                    aCAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAagag                                >  2:1242391/1‑140 (MQ=255)

                                                                                                            ttGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGtata                        <  2:1263496/140‑1 (MQ=255)

                                                                                                               ccccATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAAcg                     >  1:658146/1‑140 (MQ=255)

                                                                                                                cccATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCa                                    <  1:1183530/124‑1 (MQ=255)

                                                                                                                cccATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCa                                    >  2:1183530/1‑124 (MQ=255)

                                                                                                                  cATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGa                  >  1:405094/1‑140 (MQ=255)

                                                                                                                    tataGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGatt                >  1:718856/1‑140 (MQ=255)

                                                                                                                         tCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCg           >  2:28114/1‑140 (MQ=255)

                                                                                                                           aTTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCt         >  2:774187/1‑140 (MQ=255)

                                                                                                                            tttGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTc        >  1:763557/1‑140 (MQ=255)

                                                                                                                              tGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCAt      >  2:700088/1‑140 (MQ=255)

                                                                                                                                  tGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCAttttt  >  1:489779/1‑140 (MQ=255)

                                                                                                                                  tGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCAttttt  <  1:202740/140‑1 (MQ=255)

                                                                                                                                  tGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCAttttt  >  1:1047002/1‑140 (MQ=255)

CGTCGTAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGACAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCATTTTT  >  NC_000913/916101‑916370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A