Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 132 43.0 1464274 99.1% 1451095 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,292,147 (A)5→6 coding (691/909 nt) galU → UTP‑‑glucose‑1‑phosphate uridylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,1421.A93.2% 145.3 / 2.7 44coding (686/909 nt)galUUTP‑‑glucose‑1‑phosphate uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base A (18/23);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

TGCAAAGGCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGTTACAT  >  NC_000913/1292003‑1292274
                                                                                                                                            |                                                                                                                                    
tGCAAAGGCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGc                                                                                                                                       >  1:406157/1‑140 (MQ=255)
tGCAAAGGCGTTGAATTAGCGCCGGGAGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGc                                                                                                                                       >  2:601681/1‑140 (MQ=255)
 gCAAAGGCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑a                                                                                                                                     <  2:669076/140‑1 (MQ=255)
       gCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑aaaaaac                                                                                                                               >  1:582744/1‑138 (MQ=255)
       gCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑aaaaaac                                                                                                                               >  1:501077/1‑138 (MQ=255)
                        ggTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGTATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGTCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGtg                                                                                                               >  2:501082/1‑140 (MQ=255)
                         gTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGtga                                                                                                              <  1:729331/140‑1 (MQ=255)
                                       aTGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCAc                                                                                               >  2:694038/1‑140 (MQ=255)
                                        tggttggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCAc                                                                                               <  2:21235/140‑1 (MQ=255)
                                          gttggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCg                                                                                             <  1:601681/140‑1 (MQ=255)
                                           ttggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGa                                                                                            <  2:207313/140‑1 (MQ=255)
                                             ggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACg                                                                                          <  2:370847/140‑1 (MQ=255)
                                             ggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGc                                                                                         <  1:694038/140‑1 (MQ=255)
                                                     aGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGat                                                                                  <  2:406157/140‑1 (MQ=255)
                                                                 aGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGaa                                                                      <  2:501077/140‑1 (MQ=255)
                                                                 aGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGaa                                                                      <  2:582744/140‑1 (MQ=255)
                                                                            cgcCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGt                                                           >  2:715552/1‑140 (MQ=255)
                                                                                 tCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAg                                                      >  1:126677/1‑140 (MQ=255)
                                                                                  cTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGc                                                     <  2:580524/140‑1 (MQ=255)
                                                                                   tAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGcc                                                    <  1:597091/140‑1 (MQ=255)
                                                                                    aaTCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCt                                                   <  2:698868/140‑1 (MQ=255)
                                                                                      tctcGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAg                                                      >  2:536584/1‑135 (MQ=255)
                                                                                      tctcGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAg                                                      <  1:536584/135‑1 (MQ=255)
                                                                                         cGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCat                                              >  1:650093/1‑140 (MQ=255)
                                                                                           cTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGTCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCatat                                            >  2:637844/1‑140 (MQ=255)
                                                                                           cTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCatat                                            >  2:530610/1‑140 (MQ=255)
                                                                                               tgtgGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGc                                                     <  2:65917/127‑1 (MQ=255)
                                                                                               tgtgGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGc                                                     >  1:65917/1‑127 (MQ=255)
                                                                                               tgtgGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGaaa                                        >  1:611923/1‑140 (MQ=255)
                                                                                                gtgGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAg                                       >  1:430133/1‑140 (MQ=255)
                                                                                                   ggTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGa                                    <  2:611923/140‑1 (MQ=255)
                                                                                                      cGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAaga                                 >  1:38019/1‑140 (MQ=255)
                                                                                                        ttACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGa                                    >  1:240238/1‑135 (MQ=255)
                                                                                                        ttACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGa                                    <  2:240238/135‑1 (MQ=255)
                                                                                                        ttACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGc                               >  1:707130/1‑140 (MQ=255)
                                                                                                        ttACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGc                               >  1:315084/1‑140 (MQ=255)
                                                                                                          aCGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGaa                                   >  2:565363/1‑134 (MQ=255)
                                                                                                          aCGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGaa                                   <  1:565363/134‑1 (MQ=255)
                                                                                                                ttAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTg                       <  2:315084/140‑1 (MQ=255)
                                                                                                                 tAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGc                      <  1:51720/140‑1 (MQ=255)
                                                                                                                  aGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCg                     >  2:215452/1‑140 (MQ=255)
                                                                                                                    cgcgGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGt                   <  1:715552/140‑1 (MQ=255)
                                                                                                                    cgcgGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGta                  >  2:116877/1‑140 (MQ=255)
                                                                                                                          tatTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATaaa             <  1:501082/140‑1 (MQ=255)
                                                                                                                            tttGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAAtt           <  1:530610/140‑1 (MQ=255)
                                                                                                                              tGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCATCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAg         >  1:7142/1‑140 (MQ=255)
                                                                                                                                 ccGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAACAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGtt      <  2:650093/140‑1 (MQ=255)
                                                                                                                                 ccGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGtt      <  1:215452/140‑1 (MQ=255)
                                                                                                                                     tGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGTTACAt  >  1:549955/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                    
TGCAAAGGCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGTTACAT  >  NC_000913/1292003‑1292274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A