Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	132	43.0	1464274	99.1%	1451095	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,313,218	A→G	V149A (GTT→GCT)	yciC ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,313,218	0	A	G	100.0%	135.6 / NA	39	V149A (GTT→GCT)	yciC	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/25); total (14/25)

TACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAACGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAGCTGG  >  NC_000913/1313082‑1313352

tACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGa                                                                                                                                     >  1:108434/1‑140 (MQ=255)

tACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGa                                                                                                                                     >  1:195685/1‑140 (MQ=255)

  cTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGacc                                                                                                                                   <  2:66940/140‑1 (MQ=255)

     cGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGaccacc                                                                                                                                >  1:546467/1‑140 (MQ=255)

     cGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGACCAGTAAAATGGCCATGATAATTCCCGGAGCGaccacc                                                                                                                                >  2:153276/1‑140 (MQ=255)

         tGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCa                                                                                                                            <  2:256216/140‑1 (MQ=255)

         tGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCa                                                                                                                            <  1:436977/140‑1 (MQ=255)

         tGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCa                                                                                                                            <  1:410080/140‑1 (MQ=255)

         tGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGACCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCa                                                                                                                            <  1:153276/140‑1 (MQ=255)

          gCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCGCATTTTGTCCTGTACCACCATCACCGGAGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCat                                                                                                                           >  2:651508/1‑140 (MQ=255)

          gCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGac                                                                                                                                    <  1:476748/131‑1 (MQ=255)

          gCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGac                                                                                                                                    >  2:476748/1‑131 (MQ=255)

                   cGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAAt                                                                                                                  <  2:87542/140‑1 (MQ=255)

                    gCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATc                                                                                                                 <  2:546467/140‑1 (MQ=255)

                      atatTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTg                                                                                                               <  2:355990/140‑1 (MQ=255)

                         ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTAc                                                                                                            >  2:218439/1‑140 (MQ=255)

                            gCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTaa                                                                                                         >  1:302582/1‑140 (MQ=255)

                                    cAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAg                                                                                                 <  2:142179/140‑1 (MQ=255)

                                       ccGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTa                                                                                                          >  1:695556/1‑128 (MQ=255)

                                       ccGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTa                                                                                                          <  2:695556/128‑1 (MQ=255)

                                              cTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAAt                                                                                       <  2:396920/140‑1 (MQ=255)

                                               tGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATc                                                                                      >  2:420097/1‑140 (MQ=255)

                                                gCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCa                                                                                     <  2:302582/140‑1 (MQ=255)

                                                        tCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGaataaat                                                                             <  1:218439/140‑1 (MQ=255)

                                                         cGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGaataaata                                                                            >  1:709951/1‑140 (MQ=255)

                                                               aaaaaCGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTg                                                                      <  2:195685/140‑1 (MQ=255)

                                                                             tGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCa                                                        <  2:108434/140‑1 (MQ=255)

                                                                                    tACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCAc                                                 <  2:492313/140‑1 (MQ=255)

                                                                                                     ccAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCa                                >  1:268546/1‑140 (MQ=255)

                                                                                                       aGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACt                              <  1:195424/140‑1 (MQ=255)

                                                                                                        gggCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTg                             <  2:365113/140‑1 (MQ=255)

                                                                                                        gggCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTg                             <  2:268546/140‑1 (MQ=255)

                                                                                                         ggCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGa                            <  2:709951/140‑1 (MQ=255)

                                                                                                             aGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGActct                        >  1:104398/1‑140 (MQ=255)

                                                                                                                    tGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGAccc                 >  2:178916/1‑140 (MQ=255)

                                                                                                                          tGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGac           <  1:178916/140‑1 (MQ=255)

                                                                                                                               aTTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAg      <  2:4833/140‑1 (MQ=255)

                                                                                                                               aTTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAg      <  2:52354/140‑1 (MQ=255)

                                                                                                                                 tCCCGGAACGACCACCAGCACGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGCATAAATACCTTTGGCAATATCGGAGCACTGGCACCAATAGCTCGCAGCGCACTGCCTCTCTGACCCGCAGacagtagtt    <  2:97300/140‑7 (MQ=255)

                                                                                                                                   ccGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAGCTgg  <  1:651508/140‑1 (MQ=255)

TACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAACGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAGCTGG  >  NC_000913/1313082‑1313352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A