Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	142	96.2	3256224	98.9%	3220405	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,765	A→G	D394G (GAC→GGC)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,765	0	A	G	100.0%	179.2 / NA	50	D394G (GAC→GGC)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/26); total (24/26)

AGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTC  >  NC_000913/1066632‑1066893

aGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGa                                                                                                                            <  2:594008/140‑1 (MQ=255)

 gCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGAt                                                                                                                           <  2:341110/140‑1 (MQ=255)

   aaaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCTCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATgg                                                                                                                         >  2:1039540/1‑140 (MQ=255)

    aaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGa                                                                                                                            <  2:234105/136‑1 (MQ=255)

    aaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGa                                                                                                                            >  1:234105/1‑136 (MQ=255)

    aaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGt                                                                                                                        <  2:427370/140‑1 (MQ=255)

    aaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGt                                                                                                                        <  2:1034042/140‑1 (MQ=255)

           tCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGcc                                                                                                                 <  1:798586/140‑1 (MQ=255)

            cgcgATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGccc                                                                                                                >  1:682228/1‑140 (MQ=255)

             gcgATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCg                                                                                                               <  2:930015/140‑1 (MQ=255)

             gcgATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCg                                                                                                               <  1:796580/140‑1 (MQ=255)

                aTTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATg                                                                                                            >  1:886117/1‑140 (MQ=255)

                   tgatgaAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGAt                                                                                                         <  2:690869/140‑1 (MQ=255)

                   tgatgaAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGAt                                                                                                         <  1:271675/140‑1 (MQ=255)

                     atgaAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATaa                                                                                                       <  1:1526152/140‑1 (MQ=255)

                        aaaaTTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGtt                                                                                                    <  1:1095581/140‑1 (MQ=255)

                            ttGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGAt                                                                                                <  1:191936/140‑1 (MQ=255)

                                    gtgtATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGtt                                                                                        <  2:682228/140‑1 (MQ=255)

                                       tATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaa                                                                                     >  1:685813/1‑140 (MQ=255)

                                         tCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaatg                                                                                   <  2:886117/140‑1 (MQ=255)

                                            gagTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAg                                                                                >  1:927738/1‑140 (MQ=255)

                                             agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCTCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGc                                                                               <  1:1039540/140‑1 (MQ=255)

                                             agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATa                                                                                               >  1:279661/1‑124 (MQ=255)

                                             agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATa                                                                                               <  2:279661/124‑1 (MQ=255)

                                             agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGc                                                                               >  1:867105/1‑140 (MQ=255)

                                               tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                             >  2:1478553/1‑140 (MQ=255)

                                               tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                             >  1:519123/1‑140 (MQ=255)

                                               tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                             >  1:658262/1‑140 (MQ=255)

                                               tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTGTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                             >  2:1511161/1‑140 (MQ=255)

                                                 cGGAACAGTTACGTAATGCCGATGTGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTg                                                                           >  2:479247/1‑140 (MQ=255)

                                                 cGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTg                                                                           >  1:545646/1‑140 (MQ=255)

                                                    aaCAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGaaa                                                                        <  2:519123/140‑1 (MQ=255)

                                                         ttACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACa                                                                   <  2:545646/140‑1 (MQ=255)

                                                                    cGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcccc                                                        <  1:926017/140‑1 (MQ=255)

                                                                          gTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACGGAAAACTCCCCCGCgag                                                  <  2:72945/140‑1 (MQ=255)

                                                                           ttAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgaga                                                 >  2:1208075/1‑140 (MQ=255)

                                                                           ttAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgaga                                                 >  2:118351/1‑140 (MQ=255)

                                                                              aCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCcgcg                                                    <  1:718580/134‑1 (MQ=255)

                                                                              aCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCcgcg                                                    >  2:718580/1‑134 (MQ=255)

                                                                                     aGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgag                                                  <  1:805152/129‑1 (MQ=255)

                                                                                     aGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgag                                                  >  2:805152/1‑129 (MQ=255)

                                                                                        cACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGAGAGAAGCGGGGGAGTCg                                    <  2:685813/140‑1 (MQ=255)

                                                                                           cTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTg                                 >  2:1391032/1‑140 (MQ=255)

                                                                                              cgcAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCg                                    >  1:1457809/1‑134 (MQ=255)

                                                                                              cgcAGCGTGTGACGCTGGAATTAAGCGGTTGCCAGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCg                                    <  2:1457809/134‑1 (MQ=255)

                                                                                                                     aGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTc       >  1:473917/1‑140 (MQ=255)

                                                                                                                       cGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTg     <  1:1208075/140‑1 (MQ=255)

                                                                                                                         gTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGtt   >  2:564524/1‑140 (MQ=255)

                                                                                                                         gTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGtt   <  1:339/140‑1 (MQ=255)

                                                                                                                         gTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGtt   >  2:339/1‑140 (MQ=255)

                                                                                                                          ttGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTc  >  1:83667/1‑140 (MQ=255)

AGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTC  >  NC_000913/1066632‑1066893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A