Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	139	53.6	1844736	98.1%	1809686	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	915,468	C→T	A194T (GCT→ACT)	aqpZ ←	water channel AqpZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	915,468	0	C	T	98.3%	195.9 / ‑4.5	58	A194T (GCT→ACT)	aqpZ	water channel AqpZ
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (31/26); total (31/27)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGCAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTCGGTTGCGCCGTGGAT  >  NC_000913/915331‑915603

aCGCCAGGCCTTCTGCGTTAATTAATAACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTaa                                                                                                                                       >  1:685533/1‑140 (MQ=255)

 cGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAAc                                                                                                                                      >  2:898401/1‑140 (MQ=255)

       gCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGt                                                                                                                                >  1:639330/1‑140 (MQ=255)

       gCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGt                                                                                                                                >  1:602155/1‑140 (MQ=255)

         ttgcTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGtgc                                                                                                                              <  2:411881/137‑1 (MQ=255)

            cTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGtgctgc                                                                                                                           >  2:62227/1‑140 (MQ=255)

             tGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTgcg                                                                                                                          >  2:880864/1‑140 (MQ=255)

              gCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTgcgc                                                                                                                         <  1:137351/140‑1 (MQ=255)

                gTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTgcgcgc                                                                                                                       <  1:546000/140‑1 (MQ=255)

                         tCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAAc                                                                                                              >  2:837216/1‑140 (MQ=255)

                          cACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACa                                                                                                             <  2:463806/140‑1 (MQ=255)

                           aCGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAg                                                                                                            <  1:898401/140‑1 (MQ=255)

                              cTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAg                                                                                                         >  2:322767/1‑140 (MQ=255)

                               ttttCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAgt                                                                                                        >  1:851452/1‑140 (MQ=255)

                                 ttCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAgtgt                                                                                                      <  2:434877/140‑1 (MQ=255)

                                     agcagGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGt                                                                                                  <  1:880864/140‑1 (MQ=255)

                                               ggTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATa                                                                                        <  1:62227/140‑1 (MQ=255)

                                                    aTCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCg                                                                                             <  1:184157/130‑1 (MQ=255)

                                                    aTCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCg                                                                                             >  2:184157/1‑130 (MQ=255)

                                                       aGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTaa                                                                                <  2:433455/140‑1 (MQ=255)

                                                       aGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTaa                                                                                <  2:685533/140‑1 (MQ=255)

                                                       aGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTaa                                                                                <  2:522023/140‑1 (MQ=255)

                                                       aGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTaa                                                                                <  2:16116/140‑1 (MQ=255)

                                                        gACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAg                                                                               >  1:409422/1‑140 (MQ=255)

                                                             ccGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAAt                                                                          <  2:284755/140‑1 (MQ=255)

                                                              cGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATc                                                                         <  2:163619/140‑1 (MQ=255)

                                                               gATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCa                                                                        <  2:426735/140‑1 (MQ=255)

                                                                aTAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAg                                                                       >  2:368555/1‑140 (MQ=255)

                                                                 tAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCa                                                                        >  1:843324/1‑138 (MQ=255)

                                                                 tAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCa                                                                        <  2:843324/138‑1 (MQ=255)

                                                                    tGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGtt                                                                   <  2:639330/140‑1 (MQ=255)

                                                                    tGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGtt                                                                   <  2:602155/140‑1 (MQ=255)

                                                                        gccgACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAgg                                                               >  2:63996/1‑140 (MQ=255)

                                                                        gccgACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGCTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAgg                                                               >  2:292324/1‑140 (MQ=255)

                                                                         ccgACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGCAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGc                                                              <  1:830789/140‑1 (MQ=255)

                                                                                        accCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGAt                                               >  2:51544/1‑140 (MQ=255)

                                                                                         cccAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATc                                              <  1:322767/140‑1 (MQ=255)

                                                                                         cccAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATc                                              <  2:209898/140‑1 (MQ=255)

                                                                                          ccAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCAGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCg                                             >  1:178001/1‑140 (MQ=255)

                                                                                              aagaaCCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGgcgc                                         <  2:752541/140‑1 (MQ=255)

                                                                                               agaaCCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCa                                        <  1:63996/140‑1 (MQ=255)

                                                                                                   ccACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAAc                                    >  2:243097/1‑140 (MQ=255)

                                                                                                   ccACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGATGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAAc                                    >  1:413881/1‑140 (MQ=255)

                                                                                                    cacaGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAAcc                                   >  1:8634/1‑140 (MQ=255)

                                                                                                    cacaGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAAcc                                   >  1:756244/1‑140 (MQ=255)

                                                                                                           gttCTAATGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGgc                            >  1:744302/1‑140 (MQ=255)

                                                                                                                 aTGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAAt                      >  2:471688/1‑140 (MQ=255)

                                                                                                                 aTGCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAAt                      >  2:633835/1‑140 (MQ=255)

                                                                                                                   gCCCAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAAttt                    >  2:417736/1‑140 (MQ=255)

                                                                                                                      cAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTc                 >  2:721913/1‑140 (MQ=255)

                                                                                                                      cAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTc                 >  1:534374/1‑140 (MQ=255)

                                                                                                                      cAGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTc                 >  1:145761/1‑140 (MQ=255)

                                                                                                                      cAGCCGCCCTGGAAGATAGTAACCGCGGTACTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTc                 >  1:507326/1‑140 (MQ=255)

                                                                                                                       aGCCGCCCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTCg                >  1:402213/1‑140 (MQ=255)

                                                                                                                         ccgccCTGGAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTCGGt              <  2:409422/140‑1 (MQ=255)

                                                                                                                                 gAAGATAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTCGGTTGCGCCGt      >  2:872732/1‑140 (MQ=255)

                                                                                                                                     aTAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTCGGTTGCGCCGTGGAt  <  2:413881/140‑1 (MQ=255)

                                                                                                                                      tAGTAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTCGGTTGCGCCGt      <  1:799307/135‑1 (MQ=255)

ACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAAGATAGCAACCGCGGTGCTGCGCGCCGGGTTAACAGAAGTGTTAGTCACCGGAATACTAATTAAGTGAATCAGGGTTAAGGCCAGACCAATAGCGATCGGCGCAAAACCTGCCGGCGCGAATTTGTCGGTTGCGCCGTGGAT  >  NC_000913/915331‑915603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A