Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 139 53.6 1844736 98.1% 1809686 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 983,040 A→G intergenic (+146/‑35) ldtD → / → ycbK L,D‑transpeptidase LdtD/DUF882 domain‑containing protein YcbK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913983,0400AG100.0% 155.8 / NA 44intergenic (+146/‑35)ldtD/ycbKL,D‑transpeptidase LdtD/DUF882 domain‑containing protein YcbK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/18);  total (26/18)

AAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACC  >  NC_000913/982911‑983176
                                                                                                                                 |                                                                                                                                        
aaaTCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATaa                                                                                                                                >  2:403280/1‑140 (MQ=255)
    cAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACAtt                                                                                                                            <  2:522331/140‑1 (MQ=255)
        tGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGAcc                                                                                                                        <  1:356843/140‑1 (MQ=255)
            gTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTa                                                                                                                    >  1:695671/1‑140 (MQ=255)
              aataatTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGa                                                                                                                  <  1:601923/140‑1 (MQ=255)
              aataatTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGa                                                                                                                  <  1:403280/140‑1 (MQ=255)
              aataatTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGa                                                                                                                  <  1:297435/140‑1 (MQ=255)
                     aGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGa                                                                                                             >  2:153427/1‑138 (MQ=255)
                     aGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGa                                                                                                             <  1:153427/138‑1 (MQ=255)
                       cATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTAt                                                                                                         >  1:396744/1‑140 (MQ=255)
                       cATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTAt                                                                                                         >  1:826758/1‑140 (MQ=255)
                           aTTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATg                                                                                                     >  2:682111/1‑140 (MQ=255)
                                     aTTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCg                                                                                           >  2:278862/1‑140 (MQ=255)
                                                cccccGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAAtt                                                                                             <  2:539383/127‑1 (MQ=255)
                                                cccccGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAAtt                                                                                             >  1:539383/1‑127 (MQ=255)
                                                cccccGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATcgc                                                                                >  1:268524/1‑140 (MQ=255)
                                                           gctgGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCt                                                                     <  2:396744/140‑1 (MQ=255)
                                                           gctgGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCt                                                                     <  2:826758/140‑1 (MQ=255)
                                                            ctgGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTg                                                                    >  2:903325/1‑140 (MQ=255)
                                                                 gTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCt                                                               >  1:559595/1‑140 (MQ=255)
                                                                    gAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTtgg                                                            >  1:904491/1‑140 (MQ=255)
                                                                               aCGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCAc                                                 >  1:48221/1‑140 (MQ=255)
                                                                               aCGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCAc                                                 >  1:898275/1‑140 (MQ=255)
                                                                                  tCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCg                                              <  2:898275/140‑1 (MQ=255)
                                                                                         tACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTgccgc                                       >  1:267551/1‑140 (MQ=255)
                                                                                           cGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCa                                     <  2:559595/140‑1 (MQ=255)
                                                                                              gCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATcc                                  >  2:627005/1‑140 (MQ=255)
                                                                                                 gTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGc                               >  1:369350/1‑140 (MQ=255)
                                                                                                          ccTCTTGTGCGCCAGAAGTGCATGTAAACTATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTg                      >  1:135379/1‑140 (MQ=255)
                                                                                                            tctTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCg                    >  2:555003/1‑140 (MQ=255)
                                                                                                             ctTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGt                   <  2:625118/140‑1 (MQ=255)
                                                                                                                gtgCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTg                >  2:67540/1‑140 (MQ=255)
                                                                                                                 tgCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTg                                >  1:188013/1‑123 (MQ=255)
                                                                                                                 tgCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTg                                <  2:188013/123‑1 (MQ=255)
                                                                                                                 tgCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGc               >  1:376346/1‑140 (MQ=255)
                                                                                                                  gcgcCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCa              <  1:278862/140‑1 (MQ=255)
                                                                                                                   cgcCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCaa             >  2:883058/1‑140 (MQ=255)
                                                                                                                    gcCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAac            <  1:67540/140‑1 (MQ=255)
                                                                                                                     ccAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAaca           <  1:444001/140‑1 (MQ=255)
                                                                                                                        gAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACActc        >  1:797696/1‑140 (MQ=255)
                                                                                                                          aGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACActctc      <  1:283090/140‑1 (MQ=255)
                                                                                                                           gTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAac            >  2:176986/1‑133 (MQ=255)
                                                                                                                           gTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAac            <  1:176986/133‑1 (MQ=255)
                                                                                                                              cATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTAcc  >  1:542537/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                        
AAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACC  >  NC_000913/982911‑983176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A