Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 139 53.6 1844736 98.1% 1809686 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,207,903 T→C W135R (TGG→CGG)  ycfK → e14 prophage; protein StfP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,207,9030TC100.0% 209.2 / NA 58W135R (TGG→CGG) ycfKe14 prophage; protein StfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

TGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTG  >  NC_000913/1207769‑1208037
                                                                                                                                      |                                                                                                                                      
tgCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCggggg                                                                                                                                   >  2:258840/1‑140 (MQ=255)
    cttcattttcataaacgTTGGTTTGGGGGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGa                                                                                                                               <  1:906004‑M1/123‑1 (MQ=255)
        attttcataaacgTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGc                                                                                                                           <  2:540788‑M1/127‑1 (MQ=255)
          tctcGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCg                                                                                                                         >  2:189503/1‑140 (MQ=255)
              ataaacgTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCaaa                                                                                                                     <  1:257521‑M1/133‑1 (MQ=255)
                       GGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGc                                                                                                            <  1:687494‑M1/140‑1 (MQ=255)
                       GGTTTGGGAGA‑GGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGcc                                                                                                           <  1:267378‑M1/140‑1 (MQ=255)
                              GAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTggg                                                                                                     <  1:141259‑M1/140‑1 (MQ=255)
                                GAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTggggg                                                                                                   <  2:231704‑M1/140‑1 (MQ=255)
                                    GAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGAt                                                                                               >  1:320798‑M1/1‑140 (MQ=255)
                                        aaaaCTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGaa                                                                                           >  2:187738/1‑140 (MQ=255)
                                        aaaaCTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGaa                                                                                           <  1:893103/140‑1 (MQ=255)
                                               aaTGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGt                                                                                    >  2:518590/1‑140 (MQ=255)
                                                     gcaACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTAtt                                                                              <  1:347001/140‑1 (MQ=255)
                                                     gcaACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTAtt                                                                              <  2:687494/140‑1 (MQ=255)
                                                      caACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTc                                                                             >  2:728181/1‑140 (MQ=255)
                                                          ggCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCgg                                                                         <  1:187738/140‑1 (MQ=255)
                                                            cTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGtt                                                                       <  1:518590/140‑1 (MQ=255)
                                                               cATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCg                                                                    <  2:286311/140‑1 (MQ=255)
                                                               cATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCg                                                                    <  1:777587/140‑1 (MQ=255)
                                                                  tAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGttt                                                                 <  1:861174/140‑1 (MQ=255)
                                                                    ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGtt                                                                       <  1:403869/132‑1 (MQ=255)
                                                                    ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGtt                                                                       >  2:403869/1‑132 (MQ=255)
                                                                    ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                               <  1:133715/140‑1 (MQ=255)
                                                                    ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                               >  1:369949/1‑140 (MQ=255)
                                                                     gACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaat                                                              <  1:231704/140‑1 (MQ=255)
                                                                        gCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTaataat                                                           <  1:258840/140‑1 (MQ=255)
                                                                         cACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATg                                                          >  1:853222/1‑140 (MQ=255)
                                                                          aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                               >  1:450009/1‑134 (MQ=255)
                                                                          aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                               <  2:450009/134‑1 (MQ=255)
                                                                          aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGc                                                         <  1:540788/140‑1 (MQ=255)
                                                                                            ccGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGc                                       >  1:755610/1‑140 (MQ=255)
                                                                                               gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                                    >  2:700678/1‑140 (MQ=255)
                                                                                               gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                                    >  1:411748/1‑140 (MQ=255)
                                                                                               gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                                    <  2:755610/140‑1 (MQ=255)
                                                                                                 tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                        >  1:282326/1‑134 (MQ=255)
                                                                                                 tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                        <  2:282326/134‑1 (MQ=255)
                                                                                                 tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAAtt                                  <  2:906004/140‑1 (MQ=255)
                                                                                                    gATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGTAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATg                               >  2:138140/1‑140 (MQ=255)
                                                                                                    gATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATg                               >  2:192584/1‑140 (MQ=255)
                                                                                                         gtggtATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAAt                          >  1:639864/1‑140 (MQ=255)
                                                                                                         gtggtATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAAt                          >  1:120444/1‑140 (MQ=255)
                                                                                                              aTTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAg                     >  2:483863/1‑140 (MQ=255)
                                                                                                               ttGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGTAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgc                    <  1:138140/140‑1 (MQ=255)
                                                                                                                tGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcg                   <  2:188899/140‑1 (MQ=255)
                                                                                                                tGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcg                   <  2:444235/140‑1 (MQ=255)
                                                                                                                tGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcg                   <  2:766275/140‑1 (MQ=255)
                                                                                                                    cAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc               >  2:214377/1‑140 (MQ=255)
                                                                                                                    cAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc               <  2:141259/140‑1 (MQ=255)
                                                                                                                    cAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc               >  1:402165/1‑140 (MQ=255)
                                                                                                                    cAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc               >  1:191989/1‑140 (MQ=255)
                                                                                                                       tCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                 <  1:780364/135‑1 (MQ=255)
                                                                                                                       tCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                 >  2:780364/1‑135 (MQ=255)
                                                                                                                       tCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTAt            >  2:354833/1‑140 (MQ=255)
                                                                                                                           tAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAAt        >  1:169087/1‑140 (MQ=255)
                                                                                                                           tAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAAt        >  1:124633/1‑140 (MQ=255)
                                                                                                                            aaTCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGt                        <  1:446451/123‑1 (MQ=255)
                                                                                                                            aaTCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGt                        >  2:446451/1‑123 (MQ=255)
                                                                                                                                 ttCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTg  >  2:221713/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTG  >  NC_000913/1207769‑1208037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A