Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	139	53.6	1844736	98.1%	1809686	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,328,639	T→C	G158G (GGA→GGG)	yciK ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,328,639	0	T	C	100.0%	123.8 / NA	35	G158G (GGA→GGG)	yciK	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/18); total (17/18)

CACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTG  >  NC_000913/1328506‑1328770

cACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGa                                                                                                                               <  2:46055/140‑1 (MQ=255)

    cATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTc                                                                                                                           >  2:635929/1‑140 (MQ=255)

       tGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCaa                                                                                                                        >  1:575466/1‑140 (MQ=255)

               gcgTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTtgat                                                                                                                <  2:854160/140‑1 (MQ=255)

                cgTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCGCCTGACGTCCAACGCTtgatg                                                                                                               <  2:775020/140‑1 (MQ=255)

                     cGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTa                                                                                                          <  2:575466/140‑1 (MQ=255)

                          gggTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGAc                                                                                                     >  1:129249/1‑140 (MQ=255)

                          gggTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGAc                                                                                                     <  2:115781/140‑1 (MQ=255)

                           ggTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTtgatg                                                                                                               >  2:727926/1‑129 (MQ=255)

                           ggTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTtgatg                                                                                                               <  1:727926/129‑1 (MQ=255)

                           ggTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGAcc                                                                                                    >  1:12980/1‑140 (MQ=255)

                                aTGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGa                                                                                               <  1:550728/140‑1 (MQ=255)

                                     gTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCgg                                                                                          >  2:745232/1‑140 (MQ=255)

                                     gTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCgg                                                                                          >  2:160593/1‑140 (MQ=255)

                                      ttGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGc                                                                                         >  2:363560/1‑140 (MQ=255)

                                        gACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGt                                                                                       <  2:393040/140‑1 (MQ=255)

                                               aGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGAttt                                                                                <  1:363560/140‑1 (MQ=255)

                                                ggCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTg                                                                               >  2:297800/1‑140 (MQ=255)

                                                   gctgctGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGt                                                                            >  1:646567/1‑140 (MQ=255)

                                                   gctgctGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGt                                                                            >  1:567878/1‑140 (MQ=255)

                                                           tatTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGtaat                                                                         >  1:644308/1‑135 (MQ=255)

                                                           tatTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGtaat                                                                         <  2:644308/135‑1 (MQ=255)

                                                              tCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAg                                                                 <  1:50489/140‑1 (MQ=255)

                                                                          aCCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGt                                                     <  2:567878/140‑1 (MQ=255)

                                                                                    tcCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAg                                           >  2:461637/1‑140 (MQ=255)

                                                                                           ggTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCAt                                    <  1:635929/140‑1 (MQ=255)

                                                                                                cAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACg                               >  2:506118/1‑140 (MQ=255)

                                                                                                  aaTTTCGACGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGtt                             <  1:506118/140‑1 (MQ=255)

                                                                                                         aCGCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGc                      >  2:691939/1‑140 (MQ=255)

                                                                                                           gCTGCATATGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCAt                    <  2:815044/140‑1 (MQ=255)

                                                                                                                atatGCACCCCAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGt               <  2:713812/140‑1 (MQ=255)

                                                                                                                       ccccAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCa        <  2:129249/140‑1 (MQ=255)

                                                                                                                         ccAGTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGa      >  2:471140/1‑140 (MQ=255)

                                                                                                                            gTTGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCt   >  1:680182/1‑140 (MQ=255)

                                                                                                                             ttGGCTCGCCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTg  <  1:160593/140‑1 (MQ=255)

CACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTG  >  NC_000913/1328506‑1328770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A