Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	139	53.6	1844736	98.1%	1809686	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,516,041	C→T	A155V (GCC→GTC)	patD →	gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,516,041	0	C	T	100.0%	168.7 / NA	49	A155V (GCC→GTC)	patD	gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/22); total (27/22)

TTTTCGCGGGTGCGGCGCGCTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGCCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACA  >  NC_000913/1515906‑1516172

ttttcgcGGGTGCGGCGCGCTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTcgcg                                                                                                                                 <  2:827005/140‑1 (MQ=255)

   tcgcGGGTGCGGCGCGCTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTgg                                                                                                                              <  1:882337/140‑1 (MQ=255)

                   cTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGgcgc                                                                                                              >  2:740173/1‑140 (MQ=255)

                   cTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCAATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGgcgc                                                                                                              <  2:102551/140‑1 (MQ=255)

                      tCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTgg                                                                                                           >  2:720928/1‑140 (MQ=255)

                           aTGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCa                                                                                                      <  2:357760/140‑1 (MQ=255)

                              gTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAggg                                                                                                   <  1:163664/140‑1 (MQ=255)

                                cTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGaa                                                                                                 >  1:118197/1‑140 (MQ=255)

                                      gcAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGt                                                                                           <  1:720928/140‑1 (MQ=255)

                                         ggTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGt                                                                                        >  1:259825/1‑140 (MQ=255)

                                                 tCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAAc                                                                                >  1:63429/1‑140 (MQ=255)

                                                  cTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAAcc                                                                               >  2:615533/1‑140 (MQ=255)

                                                    tGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCAt                                                                             >  1:238829/1‑140 (MQ=255)

                                                     gAAGGTCATGCTTCGATGATCCGTCGCGATCCGTTGGGCGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATc                                                                            <  2:769232/140‑1 (MQ=255)

                                                           cATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAAt                                                                      >  1:81387/1‑140 (MQ=255)

                                                            aTACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAAtt                                                                     >  1:331832/1‑140 (MQ=255)

                                                               cTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTAcc                                                                  >  2:2794/1‑140 (MQ=255)

                                                                   gatgatCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGc                                                              >  2:205069/1‑140 (MQ=255)

                                                                    atgatCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCt                                                             <  2:81387/140‑1 (MQ=255)

                                                                    atgatCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTAACCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCt                                                             <  1:62916/140‑1 (MQ=255)

                                                                      gatCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGa                                                           <  1:2794/140‑1 (MQ=255)

                                                                          cGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACcgc                                                       >  1:148365/1‑140 (MQ=255)

                                                                          cGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACcgc                                                       >  2:153725/1‑140 (MQ=255)

                                                                            tCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGt                                                     >  1:607146/1‑140 (MQ=255)

                                                                                 aTCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAg                                                >  1:531048/1‑140 (MQ=255)

                                                                                  tCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGt                                               >  2:101277/1‑140 (MQ=255)

                                                                                          gggTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCagag                                       <  1:153725/140‑1 (MQ=255)

                                                                                            gTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCt                                     >  1:251749/1‑140 (MQ=255)

                                                                                            gTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCt                                     >  2:296175/1‑140 (MQ=255)

                                                                                              cGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCagag                                       >  1:335505/1‑136 (MQ=255)

                                                                                              cGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCagag                                       <  2:335505/136‑1 (MQ=255)

                                                                                                             aCCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTcc                    >  2:644669/1‑140 (MQ=255)

                                                                                                                gTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCgg                 >  1:585029/1‑140 (MQ=255)

                                                                                                                 tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  1:657243/1‑140 (MQ=255)

                                                                                                                 tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  2:10573/1‑140 (MQ=255)

                                                                                                                 tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  2:731070/1‑140 (MQ=255)

                                                                                                                 tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  2:584642/1‑140 (MQ=255)

                                                                                                                 tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  2:197039/1‑140 (MQ=255)

                                                                                                                     aTTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGc            <  2:607146/140‑1 (MQ=255)

                                                                                                                     aTTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGc            <  2:251749/140‑1 (MQ=255)

                                                                                                                       tATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGt          <  2:259825/140‑1 (MQ=255)

                                                                                                                       tATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGt          <  1:205069/140‑1 (MQ=255)

                                                                                                                         tCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGa        >  2:666218/1‑140 (MQ=255)

                                                                                                                          ccGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGAt       <  2:657243/140‑1 (MQ=255)

                                                                                                                               gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  2:120701/140‑1 (MQ=255)

                                                                                                                               gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  1:615533/140‑1 (MQ=255)

                                                                                                                               gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  2:118197/140‑1 (MQ=255)

                                                                                                                               gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  2:238829/140‑1 (MQ=255)

                                                                                                                               gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTAGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  2:331832/140‑1 (MQ=255)

TTTTCGCGGGTGCGGCGCGCTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGCCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACA  >  NC_000913/1515906‑1516172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A